FastQCFastQC Report
Mon 15 Aug 2016
SRR2921122_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921122_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2313293
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68250.027.7409251
CATGGGG55300.025.611344
TACATGG102550.023.8493882
TCAACGC77300.023.6341174
GTACATG107450.023.5493551
TATCAAC77450.023.5112
ATCAACG79050.023.2609623
CAACGCA79100.023.2452555
AACGCAG80150.022.8655016
ACATGGG104900.022.5209183
GGTATCA52150.022.4909841
TAGCCCT21700.021.664464
GTATAGG5400.020.9486641
CGCAGAG87750.020.7499588
ACGCAGA88950.020.2689027
GTGTAGC19600.020.0486151
ATGGGGA33250.020.0437375
GTATAAT15200.019.584971
GTAGCCC18850.017.9942863
GTATTAG8400.017.7197361
Produced by FastQC (version 0.11.5)