##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921122_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2313293
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.286465225114156	31.0	30.0	34.0	23.0	34.0
2	29.346538894986498	31.0	30.0	34.0	23.0	34.0
3	29.40385632083787	31.0	30.0	34.0	23.0	34.0
4	32.82158161547197	35.0	33.0	37.0	25.0	37.0
5	32.9039697954388	37.0	35.0	37.0	26.0	37.0
6	32.857316388369306	37.0	35.0	37.0	25.0	37.0
7	32.81417961321804	37.0	33.0	37.0	25.0	37.0
8	32.739945610002714	37.0	33.0	37.0	25.0	37.0
9	34.06521352893905	38.0	34.0	39.0	25.0	39.0
10-11	34.03021817815556	38.0	34.0	39.0	25.0	39.0
12-13	34.002585707906434	38.0	34.0	39.0	25.0	39.0
14-15	35.0076927133744	39.0	34.0	41.0	24.0	41.0
16-17	34.9147044926864	39.0	34.0	41.0	23.5	41.0
18-19	34.980247854465475	39.0	34.0	41.0	24.0	41.0
20-21	35.01104183516745	39.0	34.0	41.0	24.0	41.0
22-23	34.98018344412057	39.0	34.0	41.0	24.0	41.0
24-25	34.88153986546451	39.0	34.0	41.0	23.5	41.0
26-27	34.82064291034469	39.0	34.0	41.0	23.0	41.0
28-29	34.66873910914009	39.0	34.0	41.0	22.0	41.0
30-31	34.55768724497934	39.0	34.0	41.0	21.5	41.0
32-33	34.454972197642064	39.0	34.0	41.0	20.5	41.0
34-35	34.3454934156633	39.0	34.0	41.0	19.0	41.0
36-37	34.206215771197165	39.0	33.0	41.0	18.5	41.0
38-39	34.04226377722148	39.0	33.0	41.0	16.5	41.0
40-41	33.93578461526491	39.0	33.0	41.0	16.0	41.0
42-43	33.84767493784834	38.5	33.0	41.0	15.5	41.0
44-45	33.69114158906805	38.0	33.0	40.5	14.5	41.0
46-47	33.462757635976075	38.0	33.0	40.0	12.0	41.0
48-49	33.328448882177916	38.0	33.0	40.0	9.0	41.0
50-51	33.17192525979199	38.0	32.0	40.0	8.5	41.0
52-53	32.97506865753711	38.0	32.0	40.0	7.5	41.0
54-55	32.739237744635034	38.0	32.0	40.0	6.5	41.0
56-57	32.47615758142181	37.5	31.0	40.0	2.0	41.0
58-59	32.26624794178688	37.0	31.0	40.0	2.0	41.0
60-61	31.988754991261374	37.0	31.0	40.0	2.0	41.0
62-63	31.72805498482034	36.0	31.0	40.0	2.0	41.0
64-65	31.420508124133	36.0	30.0	39.0	2.0	41.0
66-67	31.144556915185408	35.0	30.0	39.0	2.0	40.5
68-69	30.784055456874682	35.0	30.0	39.0	2.0	40.0
70-71	30.4602901577967	35.0	29.0	38.0	2.0	40.0
72-73	30.104317092560258	35.0	29.0	37.0	2.0	39.5
74-75	30.034481797161014	35.0	29.0	37.0	2.0	39.0
76-77	29.771138805157843	35.0	29.0	36.5	2.0	39.0
78-79	29.493763219791006	34.5	29.0	36.0	2.0	39.0
80-81	29.128103746477425	34.0	29.0	36.0	2.0	37.5
82-83	28.858128650369842	34.0	29.0	35.0	2.0	37.0
84-85	28.647477211058003	34.0	29.0	35.0	2.0	36.5
86-87	28.417101508542153	34.0	29.0	35.0	2.0	36.0
88-89	28.244662262843487	34.0	28.5	35.0	2.0	36.0
90-91	28.068890754435344	34.0	28.0	35.0	2.0	35.5
92-93	28.23460344193321	34.0	29.0	35.0	2.0	35.0
94-95	28.206285801236593	34.0	29.0	35.0	2.0	35.0
96-97	28.130519998979807	34.0	29.0	35.0	2.0	35.0
98-99	28.01121301970827	34.0	29.0	35.0	2.0	35.0
100-101	27.894802992962845	34.0	29.0	35.0	2.0	35.0
102-103	26.56169430331566	32.0	25.0	34.0	2.0	35.0
104-105	27.57246336715669	34.0	27.0	35.0	2.0	35.0
106-107	27.731565780901946	34.0	27.5	35.0	2.0	35.0
108-109	27.665479686317298	34.0	27.5	35.0	2.0	35.0
110-111	27.629991099268445	34.0	27.5	35.0	2.0	35.0
112-113	27.531373241521933	34.0	27.0	35.0	2.0	35.0
114-115	27.377694697558848	34.0	27.0	35.0	2.0	35.0
116-117	27.19414228980073	34.0	27.0	35.0	2.0	35.0
118-119	27.053340627408634	34.0	27.0	35.0	2.0	35.0
120-121	26.844652406763863	34.0	25.0	35.0	2.0	35.0
122-123	26.659101549176867	34.0	25.0	35.0	2.0	35.0
124-125	25.578354536152574	33.0	21.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	108397.0
3	14933.0
4	9865.0
5	9183.0
6	11169.0
7	11758.0
8	11730.0
9	12711.0
10	13651.0
11	14716.0
12	15346.0
13	15306.0
14	15577.0
15	14078.0
16	11666.0
17	10549.0
18	9595.0
19	9553.0
20	9740.0
21	10472.0
22	12457.0
23	16194.0
24	21704.0
25	27915.0
26	32078.0
27	36808.0
28	43490.0
29	51271.0
30	60428.0
31	72183.0
32	88903.0
33	112120.0
34	148144.0
35	208770.0
36	342037.0
37	517347.0
38	181303.0
39	146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.31124223495008	19.095144572684326	13.019501306431797	24.574111885933796
2	15.834535555885083	20.580853987999532	39.266008005786226	24.31860245032915
3	19.162788646419244	24.72409235301824	29.356227740927636	26.75689125963488
4	12.785287044619487	16.41436567761105	36.95576791537353	33.84457936239594
5	13.625804945429076	37.90864423303481	34.66906177787289	13.796489043663223
6	32.521223948594745	36.388939206794774	17.433144075560154	13.656692769050323
7	27.853288653683283	32.582048274600865	21.32141895213222	18.243244119583636
8	25.825201752971445	35.3720827581675	20.20391673342409	18.598798755436967
9	26.19760420796156	16.28600788798252	19.66755996937495	37.84882793468097
10-11	24.817312811697686	26.64348215561479	27.94196897284675	20.597236059840768
12-13	25.189879683603394	24.047823935702137	27.774366064382168	22.987930316312305
14-15	22.670777512809824	25.61928572787311	25.39927490242397	26.3106618568931
16-17	22.748938004449236	28.40162292764682	26.149680034051304	22.699759033852644
18-19	22.597009021355333	27.680764853569144	27.529821702049933	22.192404423025586
20-21	23.840568274919853	26.805096488706052	27.09669190843787	22.25764332793622
22-23	23.169479864143412	26.6503240460242	26.898900191057784	23.281295898774605
24-25	22.95632693927169	26.812239212722872	27.195238778689845	23.036195069315593
26-27	22.765302419219744	27.06334437789655	27.029279080167797	23.14207412271591
28-29	22.78095879596684	27.34629874458826	27.104061951746328	22.768680507698573
30-31	23.15658732150473	26.969594982364743	27.027676669816092	22.84614102631444
32-33	22.75574442147264	26.907895803365218	27.02234653173225	23.31401324342989
34-35	23.0483449768265	27.074509925988753	26.94961230507444	22.92753279211031
36-37	23.163090504481506	26.76837644434405	27.029431060030458	23.039101991143987
38-39	22.837558191862282	27.199921495832925	26.907497796393216	23.055022515911574
40-41	23.112605297758165	26.95479953304025	26.955664113201212	22.976931056000367
42-43	22.990475148864302	26.857888494808957	27.01104867169318	23.140587684633555
44-45	23.09721100657261	26.969794694531817	26.849832874812602	23.083161424082974
46-47	22.906226547558525	27.00793967815901	26.789929240633718	23.29590453364874
48-49	22.74287171739114	26.966463595855583	27.070409048809402	23.220255637943872
50-51	22.760970053033226	27.08005996094109	26.972199436055895	23.186770549969793
52-53	23.029493827064424	26.94398403570524	26.742830809082978	23.283691328147356
54-55	23.034265978836828	26.901017608311367	26.734708964195868	23.330007448655934
56-57	22.898620705903408	27.096353893669445	26.64571056676358	23.359314833663568
58-59	22.85355047299725	26.893102146914245	26.931662997189644	23.32168438289887
60-61	22.939304320534266	26.886669897452105	27.04815873330154	23.125867048712088
62-63	22.72837213665507	26.83484597588951	27.094721202061795	23.34206068539362
64-65	22.715151583271748	26.86775314115659	27.015016640364724	23.402078635206934
66-67	22.820855326010367	27.062210636890637	26.67343198156722	23.443502055531777
68-69	23.14865709296437	26.71546244639298	26.829652611600192	23.30622784904246
70-71	22.874661679780157	26.50831054089303	26.96673146679566	23.650296312531154
72-73	22.94600670052956	26.568031989624984	27.04588782016643	23.440073489679023
74-75	22.947532828861945	26.83419168687816	26.794723346604005	23.423552137655886
76-77	22.779174445243466	26.639272242947083	27.08689029629164	23.494663015517816
78-79	23.077368663132305	26.644801684874516	26.98885741853154	23.288972233461635
80-81	22.95006246514704	26.682711635808744	26.87101487491841	23.496211024125813
82-83	22.99525459923464	26.585548520128775	27.067156444764333	23.352040435872247
84-85	23.17887608217412	26.59041284615847	26.82804049482921	23.4026705768382
86-87	22.84926167829586	26.3821782597663	27.023732896091147	23.744827165846694
88-89	23.12939741286444	26.48310286812041	26.83156730754267	23.555932411472483
90-91	22.96672145905539	26.4935356869204	27.044074911029913	23.495667942994302
92-93	22.942588034303313	26.355584317448294	27.099387613768705	23.602440034479688
94-95	22.825556697040046	26.543657242827184	27.068932238401615	23.56185382173115
96-97	22.882247068707674	26.28273421429348	27.223069146877055	23.611949570121794
98-99	22.825721625363258	26.37319289706011	26.99826111766149	23.802824359915142
100-101	22.619337870645115	26.46187378526619	27.19808461118143	23.72070373290727
102-103	22.57617509989778	26.268248488753542	27.368134600848727	23.787441810499953
104-105	22.574221300747602	26.368682697414254	27.16915373310701	23.887942268731134
106-107	22.451273917222778	26.492000879316418	27.152914810710282	23.903810392750525
108-109	22.345966328825078	26.602182344530974	27.04331615375071	24.00853517289324
110-111	22.301589566137675	26.602535835274466	27.129494648702774	23.966379949885088
112-113	22.433502072859163	26.810693273042457	27.055329560830526	23.700475093267855
114-115	22.323856073382153	26.882226884453285	26.951893758773203	23.842023283391356
116-117	22.22682135308976	26.78832580636934	27.148988928511063	23.835863912029843
118-119	22.403423030975826	26.714028163144704	26.919970267216797	23.96257853866267
120-121	22.32059792908822	26.942798324733353	26.90663604797543	23.829967698202996
122-123	22.336766268472704	26.857666912493873	26.923806823045048	23.88175999598838
124-125	22.35341556244043	26.903359815726503	26.889709766642568	23.853514855190504
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	654.0
1	481.5
2	253.5
3	184.5
4	149.0
5	125.5
6	131.0
7	163.0
8	197.5
9	239.5
10	268.5
11	275.0
12	306.0
13	355.0
14	416.5
15	468.0
16	531.0
17	659.5
18	808.5
19	963.0
20	1182.5
21	1397.0
22	1730.0
23	2275.5
24	2910.0
25	3779.5
26	5264.5
27	7365.5
28	9682.0
29	12948.5
30	16848.0
31	21827.5
32	28005.0
33	34286.0
34	40860.5
35	47606.0
36	55543.0
37	62185.5
38	66763.5
39	70542.0
40	75185.5
41	80326.0
42	82743.0
43	86155.5
44	90062.5
45	91424.0
46	92457.0
47	93930.5
48	95052.0
49	95631.0
50	96945.5
51	95757.0
52	90252.0
53	85344.0
54	81023.5
55	73994.0
56	65463.5
57	57700.5
58	52945.5
59	48913.0
60	41410.0
61	33142.0
62	25996.5
63	19394.0
64	14578.0
65	11419.5
66	8518.5
67	6368.5
68	4945.5
69	3798.5
70	2957.0
71	2207.5
72	1673.5
73	1399.5
74	1091.0
75	806.0
76	581.0
77	354.0
78	237.0
79	168.0
80	117.5
81	91.0
82	70.0
83	56.5
84	44.0
85	31.5
86	21.0
87	16.0
88	17.0
89	17.5
90	11.5
91	8.0
92	10.5
93	11.0
94	10.0
95	10.5
96	9.0
97	7.5
98	12.0
99	15.5
100	28.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0714565772688544
2	0.009337338590485512
3	0.020403813956986858
4	0.01932310347197696
5	0.010720648011298179
6	0.009596709106887886
7	0.010115450139692637
8	0.00829985652487601
9	0.006657176587660966
10-11	0.011001632737400752
12-13	0.007932414959972645
14-15	0.009726394365089075
16-17	0.013508881062623716
18-19	0.007370445507767499
20-21	0.01417892156332985
22-23	0.005122567698946913
24-25	0.004538984037041568
26-27	0.003825715116935036
28-29	0.011541987979905702
30-31	0.0074136739271678945
32-33	0.0030476035677279097
34-35	0.008580841250978584
36-37	0.007586587604769477
38-39	0.0017291367760158354
40-41	0.0013616952111124704
42-43	0.0012320099529112827
44-45	0.0024640199058225654
46-47	0.004366070359439984
48-49	0.002615319373723951
50-51	0.0052090245377477045
52-53	0.005662922941451861
54-55	0.005360324005649089
56-57	0.001923664663317617
58-59	0.0028530756804261283
60-61	0.005619694522051465
62-63	0.002917918309526722
64-65	0.003998628794536619
66-67	9.942536462091054E-4
68-69	0.0020101215021184086
70-71	0.001123938904410293
72-73	0.0018588220342170233
74-75	0.0020101215021184086
76-77	0.0020965783409192004
78-79	2.1614209700197942E-4
80-81	1.2968525820118766E-4
82-83	2.161420970019794E-5
84-85	4.3228419400395885E-4
86-87	2.593705164023753E-4
88-89	4.322841940039588E-5
90-91	6.700405007061362E-4
92-93	0.0020317357118186065
94-95	0.0012320099529112827
96-97	9.294110171085117E-4
98-99	0.001102324694710095
100-101	0.0012536241626114808
102-103	0.0023127204379211797
104-105	0.003998628794536619
106-107	0.005727765570552454
108-109	0.011931043754509265
110-111	0.00903473965468274
112-113	0.002723390422224941
114-115	0.006138435554856216
116-117	0.002334334647621378
118-119	9.294110171085117E-4
120-121	0.0047118977146431516
122-123	6.484262910059383E-4
124-125	0.0841225041531704
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2313293.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.15007859212935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.08371580005718	38.844052385778845
2	13.585134139910949	14.441018944417728
3	5.414391461252493	8.63325995082372
4	2.7280631588672857	5.799870851923555
5	1.5432130982915584	4.101094872929988
6	0.9278137186325157	2.9588023238504393
7	0.6017220592573963	2.238710231810397
8	0.4282948468857597	1.8211123818065704
9	0.27869043248742076	1.3331176550622839
>10	1.291427893942179	11.689244971500882
>50	0.0720580950845084	2.6134916835416266
>100	0.04237064216217423	4.207816817247853
>500	0.00261444477348691	0.943871842673211
>1k	4.902083950286737E-4	0.37453508663299917
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.645683880079178E-5
2	0.0	0.0	0.0	0.0	8.645683880079178E-5
3	0.0	0.0	0.0	0.0	8.645683880079178E-5
4	0.0	0.0	0.0	0.0	8.645683880079178E-5
5	0.0	0.0	0.0	0.0	8.645683880079178E-5
6	4.322841940039589E-5	0.0	0.0	0.0	1.7291367760158356E-4
7	4.322841940039589E-5	0.0	0.0	0.0	1.7291367760158356E-4
8	8.645683880079178E-5	0.0	0.0	0.0	1.7291367760158356E-4
9	8.645683880079178E-5	0.0	0.0	0.0	2.1614209700197942E-4
10-11	8.645683880079178E-5	0.0	0.0	0.0	2.1614209700197942E-4
12-13	1.0807104850098971E-4	0.0	0.0	4.322841940039589E-5	3.025989358027712E-4
14-15	2.1614209700197942E-4	0.0	0.0	4.322841940039589E-5	3.4582735520316706E-4
16-17	2.1614209700197942E-4	0.0	0.0	1.0807104850098973E-4	3.8905577460356295E-4
18-19	2.593705164023753E-4	0.0	0.0	2.593705164023753E-4	4.3228419400395885E-4
20-21	3.025989358027712E-4	0.0	0.0	4.106699843037609E-4	4.538984037041568E-4
22-23	3.458273552031671E-4	0.0	0.0	6.916547104063341E-4	4.7551261340435475E-4
24-25	4.7551261340435475E-4	0.0	0.0	0.0014481520499132622	4.7551261340435475E-4
26-27	4.7551261340435475E-4	0.0	0.0	0.0028314614707259304	4.7551261340435475E-4
28-29	6.484262910059382E-4	0.0	0.0	0.006635562377960769	4.7551261340435475E-4
30-31	7.3488312980673E-4	0.0	0.0	0.019085347165274782	4.971268231045527E-4
32-33	7.56497339506928E-4	0.0	0.0	0.0482861444702422	5.835836619053445E-4
34-35	7.997257589073239E-4	0.0	0.0	0.09307078696905234	6.916547104063342E-4
36-37	8.213399686075218E-4	0.0	0.0	0.16558646051321643	6.916547104063342E-4
38-39	9.077968074083136E-4	0.0	0.0	0.28035791402126753	6.916547104063342E-4
40-41	9.077968074083136E-4	0.0	0.0	0.40556903081451423	7.3488312980673E-4
42-43	9.510252268087095E-4	0.0	0.0	0.5205133980001668	7.56497339506928E-4
44-45	9.942536462091054E-4	0.0	0.0	0.6560561070301081	8.213399686075218E-4
46-47	0.0011455531141104911	0.0	0.0	0.8151150762138648	9.510252268087095E-4
48-49	0.0012103957432110849	0.0	0.0	0.9781942884018584	0.0010374820656095013
50-51	0.0012103957432110849	0.0	0.0	1.1412735005898518	0.001123938904410293
52-53	0.0012103957432110849	0.0	0.0	1.3059089358762594	0.0014265378402130643
54-55	0.0012103957432110849	0.0	0.0	1.4915101545718592	0.0016426799372150436
56-57	0.0012968525820118767	0.0	0.0	1.7058798863784226	0.0016642941469152414
58-59	0.0012968525820118767	0.0	0.0	1.92839817524196	0.0016859083566154395
60-61	0.0012968525820118767	0.0	0.0	2.1558661181268435	0.0017291367760158354
62-63	0.0013184667917120747	0.0	0.0	2.388953755533778	0.0018155936148166272
64-65	0.0013400810014122726	0.0	0.0	2.64140772483209	0.001858822034217023
66-67	0.0013833094208126684	0.0	0.0	2.9369820424822968	0.0019452788730178149
68-69	0.0014265378402130641	0.0	0.0	3.2607845180009623	0.0019885072924182108
70-71	0.0014481520499132622	0.0	0.0	3.604731436960212	0.0020533499215188043
72-73	0.00146976625961346	0.0	0.0	3.9423670066870042	0.00231272043792118
74-75	0.00146976625961346	0.0	0.0	4.294462482703229	0.002334334647621378
76-77	0.00146976625961346	0.0	0.0	4.699166945129734	0.002334334647621378
78-79	0.00146976625961346	0.0	0.0	5.134779727427524	0.002334334647621378
80-81	0.0015994515178146477	0.0	0.0	5.587208364872067	0.0023559488573215758
82-83	0.0015994515178146477	0.0	0.0	6.066870906538861	0.0024640199058225654
84-85	0.0015994515178146477	0.0	0.0	6.549840422289783	0.0024640199058225654
86-87	0.0015994515178146477	0.0	0.0	7.07255846967937	0.0025288625349231594
88-89	0.0015994515178146477	0.0	0.0	7.654261695340797	0.002636933583424149
90-91	0.0016426799372150436	0.0	0.0	8.254315385037692	0.002636933583424149
92-93	0.0016859083566154395	2.1614209700197945E-5	0.0	8.877258522807098	0.002680162002824545
94-95	0.0016859083566154395	4.322841940039589E-5	0.0	9.519827362984284	0.002680162002824545
96-97	0.0016859083566154395	4.322841940039589E-5	0.0	10.186236676460785	0.0027882330513255347
98-99	0.0017723651954162313	4.322841940039589E-5	0.0	10.911198884015125	0.0028963040998265244
100-101	0.0019885072924182108	4.322841940039589E-5	0.0	11.675066669029821	0.00293953251922692
102-103	0.002183035179719992	4.322841940039589E-5	0.0	12.446542655858986	0.003025989358027712
104-105	0.0023559488573215758	4.322841940039589E-5	0.0	13.217780886381448	0.003025989358027712
106-107	0.0024640199058225654	4.322841940039589E-5	0.0	14.019343853113288	0.0031340604065287015
108-109	0.0024640199058225654	4.322841940039589E-5	0.0	14.8613253919845	0.0032637456647298894
110-111	0.0025072483252229615	4.322841940039589E-5	0.0	15.733069697612883	0.003285359874430087
112-113	0.002550476744623357	4.322841940039589E-5	0.0	16.6195765084665	0.003285359874430087
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6825	0.0	27.740925	1
CATGGGG	5530	0.0	25.61134	4
TACATGG	10255	0.0	23.849388	2
TCAACGC	7730	0.0	23.634117	4
GTACATG	10745	0.0	23.549355	1
TATCAAC	7745	0.0	23.511	2
ATCAACG	7905	0.0	23.260962	3
CAACGCA	7910	0.0	23.245255	5
AACGCAG	8015	0.0	22.865501	6
ACATGGG	10490	0.0	22.520918	3
GGTATCA	5215	0.0	22.490984	1
TAGCCCT	2170	0.0	21.66446	4
GTATAGG	540	0.0	20.948664	1
CGCAGAG	8775	0.0	20.749958	8
ACGCAGA	8895	0.0	20.268902	7
GTGTAGC	1960	0.0	20.048615	1
ATGGGGA	3325	0.0	20.043737	5
GTATAAT	1520	0.0	19.58497	1
GTAGCCC	1885	0.0	17.994286	3
GTATTAG	840	0.0	17.719736	1
>>END_MODULE