FastQCFastQC Report
Mon 15 Aug 2016
SRR2921122_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921122_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2313293
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24890.10759553588758537No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA77350.035.4761051
GGTATCA54550.031.7133161
TATCAAC94600.028.7499332
ATCAACG94300.028.526393
TCAACGC95350.028.2748094
CAACGCA94900.028.1659935
AACGCAG98200.027.1406546
CATGGGG57600.027.1306844
TACATGG103750.023.6849352
ACGCAGA111200.023.6467387
CGCAGAG111250.023.4767028
GTACATG108800.023.3022841
ACATGGG105100.023.1547133
GAGTACT52200.021.48484212-13
AGTACTT57250.020.88872312-13
ATGGGGA32050.020.2461935
GTATAAT14700.019.8846151
GCAGAGT124500.019.8790919
AGAGTAC109000.019.51375610-11
GTGTAGG8750.019.089231
Produced by FastQC (version 0.11.5)