FastQCFastQC Report
Mon 15 Aug 2016
SRR2921121_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921121_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2508625
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA73850.033.1366771
GTACATG141850.029.8440591
TACATGG144400.029.2194122
CATGGGG90000.028.9599654
TCAACGC86600.027.623244
ACATGGG148350.027.5588823
GGTATCA53800.027.2251591
CAACGCA89350.026.9723175
ATCAACG89800.026.7730273
AACGCAG92450.026.4514456
TATCAAC91750.026.1391472
ACGCAGA104800.022.9368927
ATGGGGA42700.021.8791075
CGCAGAG110700.021.4465318
CCCTATA4850.020.8592932
ATGGGGG47000.020.1306155
GCAGAGT115500.019.4733929
GTATATA8000.019.3508911
GTCCTAC7900.018.8421541
TACACCG4509.276846E-1118.5128525
Produced by FastQC (version 0.11.5)