##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921121_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2508625 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.250973740594947 31.0 30.0 34.0 23.0 34.0 2 29.315363595595198 31.0 30.0 34.0 23.0 34.0 3 29.374119687079574 31.0 30.0 34.0 23.0 34.0 4 32.799146544421745 35.0 33.0 37.0 25.0 37.0 5 32.869430863520854 35.0 33.0 37.0 26.0 37.0 6 32.803324530370226 37.0 33.0 37.0 25.0 37.0 7 32.78038646668992 37.0 33.0 37.0 25.0 37.0 8 32.702847974488016 37.0 33.0 37.0 25.0 37.0 9 34.00913448602322 38.0 34.0 39.0 25.0 39.0 10-11 33.97632886541432 38.0 34.0 39.0 24.5 39.0 12-13 33.93824007175245 38.0 34.0 39.0 23.5 39.0 14-15 34.931288454830835 39.0 34.0 41.0 23.5 41.0 16-17 34.82176869799193 39.0 34.0 41.0 22.5 41.0 18-19 34.877399770790774 39.0 34.0 41.0 23.0 41.0 20-21 34.911665553839256 39.0 34.0 41.0 23.0 41.0 22-23 34.87226807514077 39.0 34.0 41.0 23.0 41.0 24-25 34.779860879964126 39.0 34.0 41.0 23.0 41.0 26-27 34.70848552493896 39.0 34.0 41.0 22.5 41.0 28-29 34.55189456375504 39.0 34.0 41.0 21.5 41.0 30-31 34.44389157406946 39.0 34.0 41.0 21.0 41.0 32-33 34.34437829488266 39.0 33.5 41.0 20.0 41.0 34-35 34.23752852658329 39.0 33.0 41.0 19.0 41.0 36-37 34.08344411779361 39.0 33.0 41.0 18.0 41.0 38-39 33.90432248741841 38.0 33.0 41.0 16.0 41.0 40-41 33.779544770541634 38.0 33.0 41.0 15.5 41.0 42-43 33.66602780407594 38.0 33.0 40.5 15.0 41.0 44-45 33.487158303851714 38.0 33.0 40.0 14.0 41.0 46-47 33.23969565000748 38.0 32.0 40.0 9.0 41.0 48-49 33.08219243609547 38.0 32.0 40.0 9.0 41.0 50-51 32.901117145846825 38.0 32.0 40.0 8.0 41.0 52-53 32.67738103542777 37.5 31.5 40.0 7.0 41.0 54-55 32.40448532562659 37.0 31.0 40.0 4.0 41.0 56-57 32.12032089291942 37.0 31.0 40.0 2.0 41.0 58-59 31.872785290746922 36.0 31.0 40.0 2.0 41.0 60-61 31.55155672928397 36.0 30.0 39.5 2.0 41.0 62-63 31.25927629677612 35.5 30.0 39.0 2.0 41.0 64-65 30.929731426578307 35.0 30.0 39.0 2.0 40.5 66-67 30.64172166027206 35.0 29.5 38.5 2.0 40.0 68-69 30.27961173949873 35.0 29.0 37.5 2.0 40.0 70-71 29.959812845682396 35.0 29.0 37.0 2.0 39.5 72-73 29.616730280532163 34.0 29.0 36.5 2.0 39.0 74-75 29.55610563555733 34.0 29.0 36.0 2.0 39.0 76-77 29.32135054063481 34.0 29.0 36.0 2.0 38.5 78-79 29.077380636803028 34.0 29.0 35.5 2.0 37.0 80-81 28.74830534655439 34.0 29.0 35.0 2.0 37.0 82-83 28.52224226418855 34.0 29.0 35.0 2.0 36.5 84-85 28.358945836862823 34.0 28.5 35.0 2.0 36.0 86-87 28.162134436195124 34.0 28.0 35.0 2.0 36.0 88-89 28.01850137027256 34.0 27.5 35.0 2.0 35.0 90-91 27.873615227465244 34.0 27.0 35.0 2.0 35.0 92-93 28.06955543375355 34.0 28.5 35.0 2.0 35.0 94-95 28.06150739947182 34.0 29.0 35.0 2.0 35.0 96-97 28.005950072250734 34.0 29.0 35.0 2.0 35.0 98-99 27.904149484279237 34.0 28.5 35.0 2.0 35.0 100-101 27.800149284966864 34.0 28.0 35.0 2.0 35.0 102-103 26.44617768697992 32.0 24.5 34.0 2.0 35.0 104-105 27.482554586675967 34.0 27.0 35.0 2.0 35.0 106-107 27.657335592206884 34.0 27.0 35.0 2.0 35.0 108-109 27.600242961781852 34.0 27.0 35.0 2.0 35.0 110-111 27.5687832976232 34.0 27.0 35.0 2.0 35.0 112-113 27.481844636005782 34.0 27.0 35.0 2.0 35.0 114-115 27.34299108077134 34.0 27.0 35.0 2.0 35.0 116-117 27.17157107977478 34.0 27.0 35.0 2.0 35.0 118-119 27.04466351088744 34.0 27.0 35.0 2.0 35.0 120-121 26.854402909960633 34.0 25.0 35.0 2.0 35.0 122-123 26.68744650954208 34.0 25.0 35.0 2.0 35.0 124-125 25.608045443220888 33.0 21.5 34.5 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 116028.0 3 17004.0 4 11505.0 5 10573.0 6 11880.0 7 13322.0 8 13862.0 9 14737.0 10 15621.0 11 16909.0 12 17080.0 13 17087.0 14 16904.0 15 14999.0 16 12636.0 17 11557.0 18 10563.0 19 10103.0 20 10426.0 21 11265.0 22 13352.0 23 17863.0 24 24145.0 25 31306.0 26 36219.0 27 41988.0 28 49304.0 29 58071.0 30 68065.0 31 82233.0 32 101272.0 33 127595.0 34 169580.0 35 243196.0 36 398818.0 37 526707.0 38 144776.0 39 74.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.351530696706845 18.001368242805256 12.814252063832715 24.832848996655184 2 16.359021339431226 19.54602281606501 38.362299912932826 25.732655931570942 3 19.854601069958782 23.645786070441215 28.205111848091054 28.294501011508956 4 13.55926459442294 15.687312686914387 34.857766647914595 35.895656070748075 5 14.695818331789823 37.18854563133658 33.14565374171817 14.969982295155425 6 33.9715093172976 34.73187479344394 16.740159759272746 14.556456129985701 7 29.84225713267416 31.675707008410985 19.7037856215112 18.778250237403654 8 27.644565599578385 34.22154592923268 19.0188267955539 19.115061675635033 9 27.07078671976126 16.12690399241931 18.533262586611247 38.26904670120818 10-11 26.118202376645367 25.696952300649727 26.3454418062744 21.839403516430508 12-13 26.389868086837566 23.153940282579086 26.54640022914727 23.909791401436078 14-15 23.771658038400236 24.518522003076036 23.956276097084142 27.753543861439585 16-17 24.0887113106961 27.484216502226992 24.69194471509969 23.735127471977215 18-19 24.101958462769673 26.63459535633737 26.10164153683168 23.161804644061277 20-21 25.092104324168947 25.60765332016105 25.78755625828999 23.512686097380016 22-23 24.741519660292095 25.298743316744453 25.746240394688797 24.213496628274655 24-25 24.240461242425344 25.79655248155393 25.650889425635505 24.312096850385227 26-27 24.121624651814056 25.87936197884225 25.781675577901463 24.217337791442226 28-29 24.121290259308847 25.973322617251497 25.823564576253315 24.08182254718634 30-31 24.37457456679314 25.81503285365895 25.62904317268776 24.18134940686015 32-33 24.027694756536764 25.832434245682233 25.60427559778782 24.535595399993184 34-35 24.247160350853765 25.94177087267947 25.697058246070036 24.11401053039673 36-37 24.456810543155573 25.667189295790145 25.68855689382098 24.18744326723331 38-39 24.345095644308767 25.912110441257575 25.612938575292148 24.129855339141507 40-41 24.288865166013178 25.873058077107675 25.7257645312358 24.112312225643347 42-43 24.310438058465596 25.743406640387768 25.609009155890917 24.337146145255716 44-45 24.25716090943699 25.723086919518373 25.655738195038243 24.364013976006394 46-47 24.361336292028206 25.761514124658643 25.62115351229411 24.25599607101904 48-49 24.2566530446934 25.932089981959837 25.49169955227471 24.319557421072048 50-51 24.14722421321946 26.202165903798026 25.458618130096145 24.19199175288637 52-53 24.384355162510943 25.866969529297894 25.430096296284482 24.318579011906678 54-55 24.1475122094342 25.81995853316968 25.57973634558509 24.45279291181103 56-57 24.203780015646256 25.912829430904843 25.389566837911776 24.49382371553713 58-59 24.23831106965323 25.723798817653087 25.479894920213507 24.557995192480178 60-61 24.16559963133464 25.84041988305399 25.68795873882005 24.30602174679132 62-63 24.00426296693968 25.829358145230678 25.712419548657277 24.453959339172357 64-65 23.94250367643688 25.778071175180173 25.65261986738916 24.62680528099379 66-67 24.040084413415258 25.87969174912282 25.556703603515256 24.523520233946662 68-69 24.191255947299616 25.618607855029694 25.651116072273876 24.53902012539681 70-71 23.895535462473628 25.558612813309402 25.90669506229066 24.63915666192631 72-73 24.070698248449222 25.63118453210725 25.792630642790716 24.505486576652817 74-75 23.934580454460747 25.840461377015462 25.680589896066596 24.5443682724572 76-77 24.000405807188567 25.578529371491165 25.850934422873813 24.57013039844645 78-79 23.909721026347512 25.609178577578785 25.892600883990585 24.588499512083118 80-81 23.921894249219044 25.707972636000324 25.852234959835556 24.51789815494507 82-83 24.028940156460212 25.573710698091585 25.868892321490854 24.52845682395735 84-85 23.85759242195848 25.701636738815996 25.72433849494333 24.716432344282197 86-87 23.822839790131795 25.615437479221658 25.881599539508144 24.6801231911384 88-89 23.842827015362403 25.4527530923819 25.84619579375072 24.858224098504976 90-91 23.79137210724863 25.56495714077005 25.895856266763452 24.747814485217866 92-93 23.77230394394281 25.432146699668056 26.106252897554434 24.689296458834693 94-95 23.62837585597889 25.375422869567522 26.2004831770014 24.795718097452188 96-97 23.77119871195782 25.173148434384174 26.170193216292585 24.88545963736542 98-99 23.621794884571397 25.248972836319123 26.03494688486479 25.094285394244693 100-101 23.4485663113156 25.441431651690845 26.13524521057371 24.974756826419846 102-103 23.445010789975278 25.26203585018368 26.32999126796311 24.96296209187793 104-105 23.293022280538697 25.443062473328553 26.091110168108113 25.17280507802464 106-107 23.29838355880964 25.422418018385056 26.19881686478936 25.08038155801594 108-109 23.348364734630287 25.3425519017882 26.146425901131607 25.1626574624499 110-111 23.16679915198764 25.57938076600169 26.208712617412957 25.045107464597717 112-113 23.14864957789655 25.607702403861964 26.25847221571674 24.985175802524747 114-115 23.055801108826664 25.806659936236986 26.08672686846661 25.050812086469744 116-117 23.045523074065187 25.658979962321222 26.143418531803324 25.152078431810267 118-119 23.0804616762032 25.551084709467197 26.190166778415673 25.178286835913926 120-121 22.956212083190405 25.6046907068649 26.329815276608713 25.109281933335975 122-123 22.97810315579963 25.584254347089924 26.218511614131128 25.219130882979314 124-125 22.892503574157868 25.636371651776585 26.20745894381266 25.263665830252886 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 783.0 1 627.0 2 396.5 3 300.0 4 233.5 5 182.0 6 192.0 7 225.5 8 266.0 9 289.0 10 300.0 11 315.5 12 340.5 13 376.0 14 407.5 15 460.0 16 550.0 17 643.0 18 738.0 19 872.0 20 1012.0 21 1168.0 22 1446.0 23 1776.5 24 2189.5 25 2831.5 26 3690.5 27 4869.5 28 6346.0 29 8437.5 30 11648.0 31 16409.0 32 22191.0 33 26223.5 34 30122.0 35 34894.0 36 40436.5 37 46347.5 38 51541.0 39 55691.0 40 58926.0 41 63792.0 42 69783.5 43 76197.5 44 81962.0 45 87931.5 46 95233.5 47 100537.5 48 104409.5 49 108258.5 50 111790.0 51 112210.0 52 109039.5 53 107654.5 54 106605.5 55 102498.0 56 95200.0 57 86561.5 58 79507.5 59 71931.0 60 61968.5 61 52198.5 62 43482.5 63 33892.0 64 25316.0 65 19669.5 66 15192.0 67 11607.0 68 8885.0 69 6817.5 70 5312.5 71 4120.0 72 3155.5 73 2538.0 74 1974.5 75 1582.0 76 1193.5 77 761.5 78 504.0 79 283.5 80 173.5 81 121.5 82 89.0 83 73.0 84 48.5 85 34.5 86 36.0 87 30.5 88 21.5 89 20.0 90 18.5 91 14.5 92 10.5 93 6.5 94 5.5 95 8.0 96 9.0 97 9.5 98 11.0 99 8.0 100 10.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07015795505506005 2 0.008650156958493199 3 0.01957247496138323 4 0.018257013304100855 5 0.010842593053963824 6 0.009008919228661118 7 0.00952713139668145 8 0.0071353829288953105 9 0.005819921271612936 10-11 0.010264587174248841 12-13 0.007753251283073396 14-15 0.008092082316009767 16-17 0.012317504609098608 18-19 0.0060790273556231 20-21 0.013074891623897553 22-23 0.004663909512182969 24-25 0.0038267975484578202 26-27 0.0034879665155214505 28-29 0.009786237480691615 30-31 0.0060790273556231 32-33 0.0025312671284069958 34-35 0.006876276844885146 36-37 0.006138821067317754 38-39 0.0013353928945139268 40-41 0.0011560117594299666 42-43 8.570432009566995E-4 44-45 0.0015944989785240918 46-47 0.0034481040410583486 48-49 0.0022123673327021772 50-51 0.0044247346654043545 52-53 0.004663909512182969 54-55 0.004544322088793662 56-57 0.0014948427923663363 58-59 0.0021924360954706265 60-61 0.004663909512182969 62-63 0.0017340176391449499 64-65 0.0032687229059743883 66-67 7.573870147989436E-4 68-69 0.0011360805221984154 70-71 8.969056754198017E-4 72-73 0.0019133987742289101 74-75 0.0018934675369973591 76-77 0.0017738801136080523 78-79 3.9862474463102296E-5 80-81 1.9931237231551148E-5 82-83 0.0 84-85 3.388310329363695E-4 86-87 1.1958742338930688E-4 88-89 1.9931237231551148E-5 90-91 2.1924360954706266E-4 92-93 0.0017539488763765013 94-95 8.171807264935971E-4 96-97 4.5841845632567645E-4 98-99 7.175245403358414E-4 100-101 0.0010364243360406597 102-103 0.0018536050625342568 104-105 0.003607553938910758 106-107 0.004803428172803826 108-109 0.008351188400019931 110-111 0.006816483133190493 112-113 0.002292092281628382 114-115 0.00468384074941452 116-117 0.002032986197618217 118-119 0.001076286810503762 120-121 0.003607553938910758 122-123 8.570432009566995E-4 124-125 0.08333250286511534 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2508625.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.95575377783358 #Duplication Level Percentage of deduplicated Percentage of total 1 73.59904006780778 38.23893604047929 2 12.796660261363883 13.297202594360188 3 5.2164009411695345 8.130661287175913 4 2.672141072319518 5.553324144522763 5 1.4782381331376777 3.8401488235152783 6 0.9515499489094217 2.966309691170882 7 0.6478997753685178 2.3563484841232283 8 0.45625072419940543 1.8963880229970056 9 0.3367914860530454 1.5748429971458429 >10 1.7286895101867186 15.75754178639682 >50 0.08543628596515192 3.0209253255394466 >100 0.02912865993588208 2.5917226349958025 >500 0.0015418552901597465 0.5717326074883413 >1k 2.312782935229856E-4 0.20391556008926884 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.195874233893069E-4 2 0.0 0.0 0.0 0.0 1.195874233893069E-4 3 0.0 0.0 0.0 0.0 1.195874233893069E-4 4 0.0 0.0 0.0 0.0 1.195874233893069E-4 5 0.0 0.0 0.0 0.0 1.195874233893069E-4 6 0.0 0.0 0.0 0.0 1.195874233893069E-4 7 0.0 0.0 0.0 0.0 1.195874233893069E-4 8 0.0 0.0 0.0 0.0 1.195874233893069E-4 9 0.0 0.0 0.0 0.0 1.195874233893069E-4 10-11 0.0 0.0 0.0 0.0 1.195874233893069E-4 12-13 0.0 0.0 0.0 0.0 2.9896855847326724E-4 14-15 0.0 0.0 0.0 0.0 3.388310329363695E-4 16-17 0.0 0.0 0.0 9.965618615775574E-5 4.1855598186257414E-4 18-19 0.0 0.0 0.0 1.195874233893069E-4 4.783496935572276E-4 20-21 0.0 0.0 0.0 2.391748467786138E-4 5.580746424834321E-4 22-23 0.0 0.0 0.0 5.979371169465345E-4 5.979371169465345E-4 24-25 0.0 0.0 0.0 0.0011759429966615178 5.979371169465345E-4 26-27 0.0 0.0 0.0 0.0022123673327021772 6.377995914096367E-4 28-29 0.0 0.0 0.0 0.006318202202401714 6.377995914096367E-4 30-31 0.0 0.0 0.0 0.01841646320195326 6.377995914096367E-4 32-33 0.0 0.0 0.0 0.037829488265484074 6.377995914096367E-4 34-35 0.0 0.0 0.0 0.06607205142259207 6.377995914096367E-4 36-37 3.9862474463102296E-5 0.0 0.0 0.11021974189047784 6.377995914096367E-4 38-39 3.9862474463102296E-5 0.0 0.0 0.18358662613981763 6.377995914096367E-4 40-41 3.9862474463102296E-5 0.0 0.0 0.26857342169515175 6.377995914096367E-4 42-43 3.9862474463102296E-5 0.0 0.0 0.3452688225621605 6.377995914096367E-4 44-45 3.9862474463102296E-5 0.0 0.0 0.43190991080771335 6.577308286411878E-4 46-47 5.979371169465345E-5 0.0 0.0 0.5365090437988937 7.175245403358414E-4 48-49 7.972494892620459E-5 0.0 0.0 0.6481040410583487 7.773182520304947E-4 50-51 7.972494892620459E-5 3.9862474463102296E-5 0.0 0.7645821914395337 7.972494892620459E-4 52-53 7.972494892620459E-5 5.979371169465345E-5 0.0 0.8790672180975634 8.769744381882505E-4 54-55 7.972494892620459E-5 7.972494892620459E-5 0.0 1.0044546315212517 9.566993871144552E-4 56-57 7.972494892620459E-5 7.972494892620459E-5 0.0 1.151327918680552 9.566993871144552E-4 58-59 7.972494892620459E-5 7.972494892620459E-5 0.0 1.3144451641835668 9.566993871144552E-4 60-61 7.972494892620459E-5 7.972494892620459E-5 0.0 1.4870098161343366 9.566993871144552E-4 62-63 7.972494892620459E-5 7.972494892620459E-5 0.0 1.6675668942149584 0.0011161492849668642 64-65 7.972494892620459E-5 7.972494892620459E-5 0.0 1.8516119388111016 0.0011360805221984154 66-67 7.972494892620459E-5 7.972494892620459E-5 0.0 2.070337336190144 0.0011560117594299666 68-69 7.972494892620459E-5 7.972494892620459E-5 0.0 2.311844137724849 0.0011560117594299666 70-71 7.972494892620459E-5 7.972494892620459E-5 0.0 2.57308286411879 0.0011560117594299666 72-73 7.972494892620459E-5 7.972494892620459E-5 0.0 2.843190991080771 0.0012755991828192735 74-75 7.972494892620459E-5 7.972494892620459E-5 0.0 3.118800139518661 0.0014151178434401316 76-77 7.972494892620459E-5 7.972494892620459E-5 0.0 3.427614729184314 0.0015147740295978873 78-79 7.972494892620459E-5 7.972494892620459E-5 0.0 3.7752155065025663 0.0015147740295978873 80-81 7.972494892620459E-5 7.972494892620459E-5 0.0 4.144740644775524 0.0015546365040609894 82-83 7.972494892620459E-5 7.972494892620459E-5 0.0 4.525825900642783 0.001714086401913399 84-85 7.972494892620459E-5 7.972494892620459E-5 0.0 4.922038965568787 0.0018137425880711544 86-87 7.972494892620459E-5 7.972494892620459E-5 0.0 5.34904579201754 0.0018934675369973591 88-89 7.972494892620459E-5 7.972494892620459E-5 0.0 5.81073297124919 0.0019532612486920127 90-91 7.972494892620459E-5 7.972494892620459E-5 0.0 6.303213912003587 0.0019532612486920127 92-93 7.972494892620459E-5 7.972494892620459E-5 0.0 6.805720265085455 0.002032986197618217 94-95 7.972494892620459E-5 7.972494892620459E-5 0.0 7.323952364343016 0.0020728486720813194 96-97 7.972494892620459E-5 7.972494892620459E-5 0.0 7.866958991479397 0.002312023518859933 98-99 7.972494892620459E-5 7.972494892620459E-5 0.0 8.447994419253575 0.002391748467786138 100-101 7.972494892620459E-5 7.972494892620459E-5 0.0 9.078798146394938 0.002391748467786138 102-103 7.972494892620459E-5 7.972494892620459E-5 0.0 9.721580547112463 0.002391748467786138 104-105 7.972494892620459E-5 7.972494892620459E-5 0.0 10.375923065424285 0.002391748467786138 106-107 7.972494892620459E-5 7.972494892620459E-5 0.0 11.04092879565499 0.002391748467786138 108-109 7.972494892620459E-5 7.972494892620459E-5 0.0 11.727201156011759 0.002391748467786138 110-111 7.972494892620459E-5 7.972494892620459E-5 0.0 12.455588220638797 0.002391748467786138 112-113 7.972494892620459E-5 7.972494892620459E-5 0.0 13.2066968957098 0.002391748467786138 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7385 0.0 33.136677 1 GTACATG 14185 0.0 29.844059 1 TACATGG 14440 0.0 29.219412 2 CATGGGG 9000 0.0 28.959965 4 TCAACGC 8660 0.0 27.62324 4 ACATGGG 14835 0.0 27.558882 3 GGTATCA 5380 0.0 27.225159 1 CAACGCA 8935 0.0 26.972317 5 ATCAACG 8980 0.0 26.773027 3 AACGCAG 9245 0.0 26.451445 6 TATCAAC 9175 0.0 26.139147 2 ACGCAGA 10480 0.0 22.936892 7 ATGGGGA 4270 0.0 21.879107 5 CGCAGAG 11070 0.0 21.446531 8 CCCTATA 485 0.0 20.859293 2 ATGGGGG 4700 0.0 20.130615 5 GCAGAGT 11550 0.0 19.473392 9 GTATATA 800 0.0 19.350891 1 GTCCTAC 790 0.0 18.842154 1 TACACCG 450 9.276846E-11 18.512852 5 >>END_MODULE