FastQCFastQC Report
Mon 15 Aug 2016
SRR2921121_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921121_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2508625
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32760.13058946634112312No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27660.11025960436494095No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA89450.038.8787651
GGTATCA59850.036.578521
TCAACGC111400.030.406464
TATCAAC114200.030.0756992
ATCAACG113500.029.895823
CAACGCA114050.029.7006035
AACGCAG117900.028.8620626
GTACATG132650.027.206481
CATGGGG84300.026.1036684
TACATGG136000.026.0001072
TACCGTA2558.8584784E-1025.6624227
ACGCAGA133100.025.4319327
ACATGGG139000.025.0977693
CGCAGAG139600.024.2903968
GTATACG1602.5677745E-422.369461
GCAGAGT147650.021.9995089
GAGTACT58550.021.13483812-13
AGTACTT60600.020.95982712-13
GTACTTT60500.020.20860114-15
AGAGTAC134750.019.84556210-11
Produced by FastQC (version 0.11.5)