FastQCFastQC Report
Mon 15 Aug 2016
SRR2921120_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921120_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2569779
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49700.039.0603451
GTATCAA73200.036.7707251
TATCAAC86000.030.794582
TCAACGC86800.030.3044784
CAACGCA89950.029.374415
ATCAACG89800.029.2926483
AACGCAG93500.028.5114926
GTACATG132000.025.4436861
TACATGG130500.025.4013842
ACGCAGA103900.025.0848857
ACATGGG127700.024.9796663
CGCAGAG106450.024.148588
CATGGGG89450.023.685034
GCAGAGT114350.020.9711579
CAGAGTA109200.018.93736510-11
CCGTCTA3303.7673271E-718.0318229
AGAGTAC103800.017.715310-11
ACCGAGT7100.017.6000738
GAGTACT31300.017.01567512-13
GTATAGG4903.110472E-1017.0140151
Produced by FastQC (version 0.11.5)