FastQCFastQC Report
Mon 15 Aug 2016
SRR2921120_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921120_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2569779
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35540.13829983045234628No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32490.12643110555421302No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54950.051.14571
GTATCAA85050.040.762211
TATCAAC105550.032.493462
TCAACGC107750.031.6670444
ATCAACG109900.031.0469283
CAACGCA110900.031.0237085
AACGCAG115600.029.7947046
ACGCAGA131300.026.1867437
CGCAGAG133800.025.520618
GTACATG125150.023.2195631
GAGTACT64800.023.08998312-13
TACATGG124400.022.9668562
ACATGGG120400.022.9390163
GCAGAGT147800.022.2588589
GTACTTT66750.022.23697514-15
CAGAGTA139150.021.46233210-11
CATGGGG82600.021.448914
AGAGTAC130900.021.4288310-11
GTGGTAT33050.019.8599051
TGGTATC32900.019.2174762
Produced by FastQC (version 0.11.5)