##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921120_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2569779
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.655803864845964	31.0	31.0	34.0	27.0	34.0
2	30.77965770597394	31.0	31.0	34.0	27.0	34.0
3	30.841006561264606	31.0	31.0	34.0	27.0	34.0
4	34.35206646174632	37.0	35.0	37.0	30.0	37.0
5	33.97732022870449	37.0	35.0	37.0	30.0	37.0
6	33.83268288829506	37.0	35.0	37.0	29.0	37.0
7	33.6669768100681	37.0	35.0	37.0	28.0	37.0
8	33.66435401643488	37.0	35.0	37.0	28.0	37.0
9	35.085148567250336	39.0	35.0	39.0	28.0	39.0
10-11	35.03508492364519	39.0	35.0	39.0	27.5	39.0
12-13	34.9137240984536	39.0	35.0	39.0	27.0	39.0
14-15	36.015655820986936	40.0	36.0	41.0	27.0	41.0
16-17	35.96352176587948	40.0	36.0	41.0	27.0	41.0
18-19	36.01421094965754	40.0	36.0	41.0	27.0	41.0
20-21	36.013724526505975	40.0	36.0	41.0	27.0	41.0
22-23	35.974046406325215	40.0	36.0	41.0	27.0	41.0
24-25	35.866029919304346	40.0	36.0	41.0	27.0	41.0
26-27	35.760753551180855	39.5	35.0	41.0	27.0	41.0
28-29	35.65803927108129	39.0	35.0	41.0	27.0	41.0
30-31	35.55153556006178	39.0	35.0	41.0	26.0	41.0
32-33	35.3964739769451	39.0	35.0	41.0	25.5	41.0
34-35	35.26381101254232	39.0	35.0	41.0	25.0	41.0
36-37	35.11560546646229	39.0	35.0	41.0	25.0	41.0
38-39	34.93552558410665	39.0	34.0	41.0	24.0	41.0
40-41	34.77938141762385	39.0	34.0	41.0	24.0	41.0
42-43	34.622913682460634	39.0	34.0	41.0	23.5	41.0
44-45	34.424833419527516	38.5	33.5	41.0	22.5	41.0
46-47	34.15898273742606	38.0	33.0	41.0	20.5	41.0
48-49	33.92000498875585	38.0	33.0	41.0	18.5	41.0
50-51	33.68059393434221	38.0	33.0	40.0	17.5	41.0
52-53	33.48029071760646	38.0	33.0	40.0	15.5	41.0
54-55	33.24685624717145	38.0	32.5	40.0	13.5	41.0
56-57	32.951590973387205	37.0	32.0	40.0	12.5	41.0
58-59	32.70211815879887	37.0	32.0	40.0	9.0	41.0
60-61	32.401929309874504	36.0	31.5	40.0	8.0	41.0
62-63	32.09292102550452	36.0	31.0	39.0	7.0	41.0
64-65	31.776733524556004	35.0	31.0	39.0	6.5	41.0
66-67	31.43263681429415	35.0	31.0	39.0	2.0	40.5
68-69	31.0567091567018	35.0	30.0	38.0	2.0	40.0
70-71	30.69044419773062	35.0	30.0	37.0	2.0	39.5
72-73	30.5104946767796	35.0	30.0	37.0	2.0	39.0
74-75	30.317273391992074	35.0	30.0	36.0	2.0	39.0
76-77	30.0997537142299	35.0	30.0	36.0	2.0	39.0
78-79	29.793622330947525	35.0	30.0	35.5	2.0	37.0
80-81	29.519011557024943	34.5	30.0	35.0	2.0	37.0
82-83	29.324324971135653	34.0	29.5	35.0	2.0	36.5
84-85	29.183085004585998	34.0	29.0	35.0	2.0	36.0
86-87	29.000518916218088	34.0	29.0	35.0	2.0	36.0
88-89	28.867655545476868	34.0	29.0	35.0	2.0	35.5
90-91	28.72494833213284	34.0	29.0	35.0	2.0	35.0
92-93	28.62227432787022	34.0	29.0	35.0	2.0	35.0
94-95	28.531681323569067	34.0	29.0	35.0	2.0	35.0
96-97	28.46990772358246	34.0	29.0	35.0	2.0	35.0
98-99	28.418945559131735	34.0	29.0	35.0	2.0	35.0
100-101	28.270644674114	34.0	29.0	35.0	2.0	35.0
102-103	26.905651030691743	32.0	25.5	34.0	2.0	35.0
104-105	28.00000252940039	34.0	28.0	35.0	2.0	35.0
106-107	28.28674528043073	34.0	29.0	35.0	2.0	35.0
108-109	28.497636761760447	34.0	29.0	35.0	2.0	35.0
110-111	28.473987646408503	34.0	29.0	35.0	2.0	35.0
112-113	28.396815251428237	34.0	29.0	35.0	2.0	35.0
114-115	28.28104031513994	34.0	29.0	35.0	2.0	35.0
116-117	28.1901507872856	34.0	29.0	35.0	2.0	35.0
118-119	28.0511935073016	34.0	29.0	35.0	2.0	35.0
120-121	27.87930966048053	34.0	29.0	35.0	2.0	35.0
122-123	27.699265968007367	34.0	29.0	35.0	2.0	35.0
124-125	26.90391274891732	33.5	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	60873.0
3	20661.0
4	10710.0
5	8812.0
6	9887.0
7	12013.0
8	13639.0
9	14881.0
10	15788.0
11	17194.0
12	18255.0
13	20049.0
14	22638.0
15	19304.0
16	13716.0
17	12564.0
18	12107.0
19	11477.0
20	11320.0
21	11215.0
22	12057.0
23	14938.0
24	20202.0
25	27282.0
26	34027.0
27	39569.0
28	47450.0
29	55932.0
30	66302.0
31	80525.0
32	98657.0
33	124240.0
34	164216.0
35	237929.0
36	418526.0
37	610381.0
38	180162.0
39	281.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.8090112909859	19.262713263792826	14.098972158871401	24.829303286349873
2	17.150120711098104	20.46862523212176	36.596826488886954	25.784427567893182
3	19.37904073756132	23.76959614221684	29.217524085850616	27.63383903437122
4	14.222260515904267	16.93297236772955	33.66009453514502	35.18467258122116
5	15.095197076562656	36.2334185898714	33.02212360432659	15.649260729239359
6	33.5809545455253	34.0767677648242	16.75981001869613	15.582467670954376
7	28.61049791704289	31.51161505458033	20.402315849778006	19.475571178598784
8	27.060584915125546	33.149393598212264	19.581739800840708	20.20828168582149
9	26.46148528940133	15.443014917658635	19.12315553161479	38.97234426132525
10-11	26.084389435392204	25.382159326933312	26.372306698926895	22.161144538747592
12-13	26.540319592476003	22.97181847620649	26.54438689247382	23.943475038843687
14-15	24.006332226646848	24.244452441252815	24.265646794641118	27.48356853745922
16-17	23.809514541717057	27.010241501064396	25.29498177736033	23.885262179858213
18-19	23.884412120457238	25.57072391307944	27.074074758843224	23.470789207620097
20-21	24.969565710720207	24.706161924237254	26.67908236457739	23.64519000046515
22-23	24.577319378954495	24.530024536875477	26.630421560531264	24.26223452363876
24-25	24.311416123378162	25.012582339883156	26.665997165518977	24.010004371219704
26-27	23.75704553809996	25.21542156650379	26.947357440308156	24.080175455088092
28-29	23.73923514211685	25.401142944778947	26.957156211081422	23.90246570202278
30-31	24.107912138685126	24.94045027551783	26.891252345448542	24.06038524034851
32-33	23.943881530703276	24.906643074111038	26.646114000931036	24.503361394254647
34-35	24.06992397545526	25.179765945682632	26.68813199307385	24.062178085788258
36-37	24.224749812720816	24.88556458470342	26.707111315166003	24.182574287409757
38-39	23.8758842278509	25.240024165910185	26.668627292219107	24.21546431401981
40-41	24.103012243992346	25.00997464008579	26.968585235975656	23.918427879946204
42-43	24.240585313142997	24.896692043418923	26.637863183519926	24.224859459918147
44-45	24.11858252308897	25.07647343092012	26.49286432872803	24.31207971726288
46-47	24.286630433195956	25.153664624681905	26.256978673504637	24.3027262686175
48-49	23.974376190837628	25.434189860631857	26.17086950852343	24.420564440007084
50-51	24.033240304959712	25.5490857183897	26.20531236843985	24.212361608210735
52-53	24.37541114419727	25.317529826200346	26.013253926548725	24.29380510305366
54-55	24.252138706767994	25.49020829059553	25.80265002283026	24.455002979806217
56-57	23.979419553720543	25.76162189735818	25.730151003847546	24.528807545073736
58-59	23.891079102443413	25.548351864570524	26.05241345272262	24.508155580263438
60-61	23.914199358139733	25.763352464583832	26.108143139899457	24.214305037376977
62-63	23.850844734123427	25.601115464773095	26.246781789150063	24.30125801195341
64-65	23.792045215448976	25.646936681749256	25.99868852152699	24.56232958127478
66-67	23.732219068434215	25.889822698203506	25.82370409384521	24.554254139517067
68-69	24.040408011613877	25.456876043574226	26.094868597473216	24.407847347338677
70-71	23.84286325201407	25.416220031311028	26.040007497829194	24.700909218845712
72-73	23.91249929925316	25.385838996405948	26.02608802626088	24.675573678080013
74-75	23.836064654900614	25.707197034276003	25.79656733951296	24.660170971310418
76-77	23.881187810521688	25.4702025676604	25.895732826314138	24.752876795503774
78-79	23.981185487860557	25.640835019015746	25.852166463554422	24.525813029569278
80-81	24.049918969157677	25.56577082839075	25.765589168277327	24.61872103417425
82-83	23.89726281602036	25.486640651258398	25.94103775283234	24.6750587798889
84-85	23.855128137764883	25.55911034306943	25.778274669609097	24.807486849556593
86-87	23.923518255226384	25.395425791814873	25.79163973021645	24.88941622274229
88-89	23.76935352041412	25.515412758338847	25.616607636321177	25.098626084925858
90-91	23.513399956057103	25.63347927123295	25.85397219064678	24.999148582063164
92-93	23.680344829196827	25.395227070680022	25.98536250815443	24.939065591968724
94-95	23.610272952785994	25.25545476465195	26.059236976122396	25.07503530643966
96-97	23.769574410736404	25.042887822193855	26.020078214932457	25.167459552137288
98-99	23.55777071221425	25.32321450186804	25.741601988736875	25.37741279718083
100-101	23.384641431995117	25.213167489367166	26.033260258457368	25.368930820180342
102-103	23.330358491182896	24.97793193851768	26.189904426222615	25.501805144076805
104-105	23.396978697219346	25.10110773836653	25.917967967013446	25.58394559740068
106-107	23.282316999245527	25.248774928819284	25.857296843295003	25.61161122864019
108-109	23.191866016883136	25.235928594905715	25.828545029644445	25.743660358566707
110-111	23.114338943986997	25.159712733079402	25.899810738222122	25.82613758471148
112-113	23.11573629940397	25.32158917768208	25.88936390472095	25.673310618192996
114-115	23.099758634028944	25.495496862865057	25.702576568119923	25.702167934986083
116-117	22.909293845660194	25.457722176051046	25.900193594623484	25.73279038366528
118-119	22.9807377612186	25.397828255252975	25.662320660606415	25.95911332292201
120-121	22.85872226047808	25.57490962418957	25.51881328714612	26.047554828186243
122-123	22.789357486687518	25.526593635304746	25.587673632393766	26.096375245613974
124-125	22.775698243593435	25.55529792563788	25.585279039482984	26.083724791285697
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	41.0
1	44.5
2	96.0
3	150.0
4	135.0
5	106.0
6	138.5
7	386.0
8	629.0
9	710.0
10	672.5
11	552.5
12	547.0
13	625.5
14	738.0
15	877.5
16	974.5
17	1109.0
18	1310.5
19	1438.0
20	1551.0
21	1832.0
22	2286.0
23	2803.0
24	3377.0
25	4159.5
26	5622.0
27	7746.5
28	9711.5
29	12095.5
30	15117.5
31	19497.0
32	25173.5
33	28392.5
34	31147.0
35	35439.0
36	40100.5
37	44822.5
38	50327.5
39	54740.0
40	58281.0
41	63085.5
42	67135.5
43	73133.0
44	79629.0
45	85651.0
46	92604.5
47	96813.0
48	100169.5
49	103459.5
50	107029.0
51	111085.0
52	112429.5
53	114507.0
54	116006.0
55	110806.0
56	102313.0
57	93693.5
58	87762.0
59	80836.0
60	67870.5
61	56142.0
62	46475.0
63	34504.0
64	24789.5
65	19082.0
66	14381.5
67	11362.0
68	8750.5
69	6267.5
70	4639.0
71	3295.5
72	2303.5
73	1831.0
74	1393.5
75	1047.5
76	780.5
77	467.0
78	271.0
79	164.5
80	111.5
81	66.5
82	43.5
83	26.0
84	16.5
85	13.0
86	8.0
87	8.5
88	6.0
89	2.5
90	4.0
91	4.0
92	2.5
93	2.5
94	1.5
95	1.0
96	4.0
97	5.5
98	3.0
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.080434932342431
2	2.723969648751896E-4
3	5.058800776253522E-4
4	0.18705888716500524
5	0.07171822946642493
6	0.010234343108882125
7	0.013814417504384618
8	0.010818050890757533
9	0.01848407975938787
10-11	0.018075484312075084
12-13	0.019729323027388737
14-15	0.027181325709331423
16-17	0.02809580123426956
18-19	0.02535237465945515
20-21	0.027998516603956997
22-23	0.03068357240058386
24-25	0.02731752419176902
26-27	0.026792187188081153
28-29	0.02776503349120683
30-31	0.027531550378456667
32-33	0.023620708239891444
34-35	0.026247393258330778
36-37	0.029321587576207918
38-39	0.0338939652008986
40-41	0.0297301830235207
42-43	0.029613441467145618
44-45	0.029165932167707808
46-47	0.030800313956958945
48-49	0.02661707485351853
50-51	0.03305731738021052
52-53	0.02836819819914475
54-55	0.031909358742522216
56-57	0.02873787979433251
58-59	0.025235633103080073
60-61	0.02661707485351853
62-63	0.03132565096064681
64-65	0.03589802858533749
66-67	0.03543106235983717
68-69	0.03048900313995873
70-71	0.04029529387546556
72-73	0.042455012668404554
74-75	0.026967299522643776
76-77	0.02000171999226393
78-79	0.02303700045801604
80-81	0.027064584152956345
82-83	0.025702599328580395
84-85	0.01844516590726284
86-87	0.01790037197751246
88-89	0.018639735167887975
90-91	0.020838367812952008
92-93	0.02424332987389188
94-95	0.019009416763075736
96-97	0.02243383575007812
98-99	0.01984606458376382
100-101	0.019320727580075952
102-103	0.017705802716887327
104-105	0.019067787541263276
106-107	0.01706372415682438
108-109	0.01597413629732362
110-111	0.012530260384258725
112-113	0.010662395482257423
114-115	0.00922258295363142
116-117	0.009125298323318853
118-119	0.005447939297503793
120-121	0.003599531321565006
122-123	0.0077049427207553645
124-125	0.05819566585297802
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2569779.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.98605918159194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.99157971023276	37.48533541516764
2	13.001576014017582	12.997950961812982
3	4.602716142041742	6.9021492441650105
4	2.211112054074769	4.420991119684508
5	1.238925812016523	3.0964509480529885
6	0.7818790807099782	2.3449832400730597
7	0.5535322071556262	1.9368225566058839
8	0.3865360026619309	1.5457129203900206
9	0.2956448065715088	1.3300306918212368
>10	1.7451110284540834	15.96961493410734
>50	0.12538173572626146	4.330939696543703
>100	0.061554731969556326	5.498021151225467
>500	0.0031232802579594177	1.1168858553640715
>1k	0.001327394109629204	1.0241112649860835
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3554	0.13829983045234628	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3249	0.12643110555421302	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.891385212502709E-5
2	0.0	0.0	0.0	0.0	3.891385212502709E-5
3	0.0	0.0	0.0	0.0	3.891385212502709E-5
4	0.0	0.0	0.0	0.0	7.782770425005419E-5
5	0.0	0.0	0.0	0.0	7.782770425005419E-5
6	0.0	0.0	0.0	0.0	1.9456926062513547E-4
7	0.0	0.0	0.0	0.0	1.9456926062513547E-4
8	0.0	0.0	0.0	0.0	1.9456926062513547E-4
9	0.0	0.0	0.0	0.0	2.3348311275016257E-4
10-11	0.0	0.0	0.0	0.0	2.3348311275016257E-4
12-13	0.0	0.0	0.0	0.0	5.253370036878658E-4
14-15	0.0	0.0	0.0	3.891385212502709E-5	6.615354861254606E-4
16-17	0.0	0.0	0.0	5.8370778187540637E-5	8.366478206880825E-4
18-19	0.0	0.0	0.0	1.3619848243759484E-4	8.950185988756232E-4
20-21	0.0	0.0	0.0	1.9456926062513547E-4	9.144755249381367E-4
22-23	0.0	0.0	0.0	3.6968159518775744E-4	0.001031217081313218
24-25	0.0	0.0	0.0	7.393631903755148E-4	0.0010895878595007587
26-27	0.0	0.0	0.0	0.0016732956413761648	0.00120632941587584
28-29	0.0	0.0	0.0	0.004066497547065331	0.001245243268000867
30-31	0.0	0.0	0.0	0.013113968166134131	0.0012647001940633805
32-33	0.0	0.0	0.0	0.0324541526722726	0.0013619848243759483
34-35	0.0	0.0	0.0	0.06270967269948116	0.0014008986765009753
36-37	0.0	0.0	0.0	0.10749951649538735	0.001459269454688516
38-39	0.0	0.0	0.0	0.16900285977899265	0.001673295641376165
40-41	0.0	0.0	0.0	0.2346505283139134	0.001712209493501192
42-43	0.0	0.0	0.0	0.3053959114772126	0.001712209493501192
44-45	0.0	0.0	0.0	0.3932244757233988	0.0017705802716887326
46-47	0.0	0.0	0.0	0.49605432996378285	0.001848407975938787
48-49	0.0	0.0	0.0	0.5947982297310391	0.0019067787541263276
50-51	0.0	0.0	0.0	0.6934448448679829	0.001984606458376382
52-53	0.0	0.0	0.0	0.7968973207423673	0.0021791757190015174
54-55	0.0	0.0	0.0	0.9235813663353931	0.002295917275376599
56-57	0.0	0.0	0.0	1.0729132738651845	0.002393201905689166
58-59	0.0	0.0	0.0	1.2219922413561632	0.002451572683876707
60-61	0.0	0.0	0.0	1.3693006285754534	0.0026072280923768155
62-63	0.0	0.0	0.0	1.5221542397225598	0.0027239696487518965
64-65	0.0	0.0	0.0	1.7059054494569377	0.0027239696487518965
66-67	0.0	0.0	0.0	1.9218189579726506	0.0027628835008769235
68-69	0.0	0.0	0.0	2.154134655159062	0.0028017973530019506
70-71	0.0	0.0	0.0	2.38131372386497	0.0028017973530019506
72-73	0.0	0.0	0.0	2.6200112927998864	0.0028601681311894913
74-75	0.0	0.0	0.0	2.8843920041373208	0.002957452761502059
76-77	0.0	0.0	0.0	3.195605536507225	0.002996366613627086
78-79	0.0	0.0	0.0	3.5296420431484576	0.0030741943178771406
80-81	0.0	0.0	0.0	3.864203886793378	0.003132565096064681
82-83	1.9456926062513547E-5	0.0	0.0	4.208747133508368	0.0033271343566898164
84-85	3.891385212502709E-5	3.891385212502709E-5	0.0	4.590628221337321	0.00334659128275233
86-87	3.891385212502709E-5	3.891385212502709E-5	0.0	5.019595070237557	0.003443875913064898
88-89	3.891385212502709E-5	3.891385212502709E-5	0.0	5.4822224012259415	0.0035411605433774656
90-91	3.891385212502709E-5	3.891385212502709E-5	0.0	5.954578195245583	0.0035411605433774656
92-93	3.891385212502709E-5	3.891385212502709E-5	0.0	6.42333445794366	0.0036189882476275197
94-95	3.891385212502709E-5	3.891385212502709E-5	0.0	6.916995586001754	0.0036579020997525467
96-97	3.891385212502709E-5	3.891385212502709E-5	0.0	7.468871836838888	0.0037941005821901415
98-99	3.891385212502709E-5	3.891385212502709E-5	0.0	8.054778251359359	0.003930299064627736
100-101	3.891385212502709E-5	3.891385212502709E-5	0.0	8.672029773766539	0.003969212916752764
102-103	3.891385212502709E-5	3.891385212502709E-5	0.0	9.28688809426803	0.0040275836949403045
104-105	3.891385212502709E-5	3.891385212502709E-5	0.0	9.919218734373656	0.004085954473127845
106-107	3.891385212502709E-5	3.891385212502709E-5	0.0	10.605581258154885	0.0041054113991903585
108-109	3.891385212502709E-5	3.891385212502709E-5	0.0	11.337550816626642	0.004124868325252872
110-111	3.891385212502709E-5	3.891385212502709E-5	0.0	12.076447040776658	0.0041832391034404125
112-113	3.891385212502709E-5	3.891385212502709E-5	0.0	12.802618435281788	0.004241609881627953
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5495	0.0	51.1457	1
GTATCAA	8505	0.0	40.76221	1
TATCAAC	10555	0.0	32.49346	2
TCAACGC	10775	0.0	31.667044	4
ATCAACG	10990	0.0	31.046928	3
CAACGCA	11090	0.0	31.023708	5
AACGCAG	11560	0.0	29.794704	6
ACGCAGA	13130	0.0	26.186743	7
CGCAGAG	13380	0.0	25.52061	8
GTACATG	12515	0.0	23.219563	1
GAGTACT	6480	0.0	23.089983	12-13
TACATGG	12440	0.0	22.966856	2
ACATGGG	12040	0.0	22.939016	3
GCAGAGT	14780	0.0	22.258858	9
GTACTTT	6675	0.0	22.236975	14-15
CAGAGTA	13915	0.0	21.462332	10-11
CATGGGG	8260	0.0	21.44891	4
AGAGTAC	13090	0.0	21.42883	10-11
GTGGTAT	3305	0.0	19.859905	1
TGGTATC	3290	0.0	19.217476	2
>>END_MODULE