FastQCFastQC Report
Mon 15 Aug 2016
SRR2921119_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921119_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2919706
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61850.037.4451681
GGTATCA43850.037.2020531
GTACATG124750.030.2576081
TCAACGC75200.030.0681064
TACATGG124650.029.836162
ATCAACG77300.029.483193
CAACGCA76900.029.325025
TATCAAC77550.029.004492
ACATGGG127700.028.7973673
CATGGGG86250.028.423564
AACGCAG80650.028.0328666
ACGCAGA94400.023.6975887
CGCAGAG98550.022.5796998
GCAGAGT105150.020.370259
TATACAG16650.020.3697685
AGAGTAC94150.018.70860910-11
ATGGGGA47000.018.609945
ATGGGGG43900.018.4331635
GTATAGG8600.017.9995141
ATGGGGC35750.017.9751785
Produced by FastQC (version 0.11.5)