FastQCFastQC Report
Mon 15 Aug 2016
SRR2921119_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921119_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2919706
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32930.11278532838580321No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31390.10751082472002318No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57050.050.4045751
GTATCAA87150.041.2789341
TCAACGC109250.032.2138024
ATCAACG110950.031.7196713
TATCAAC113350.031.519512
CAACGCA112800.031.1358075
AACGCAG115800.030.461996
GTACATG128600.027.6028921
TACATGG129700.026.5804312
ACGCAGA132200.026.5030567
ACATGGG129850.025.7246843
CGCAGAG137900.025.3657388
CATGGGG86900.023.7502294
GCAGAGT151250.022.6159259
GAGTACT68350.022.32515312-13
AGAGTAC136550.022.28432810-11
GTACTTT72250.021.2849214-15
CAGAGTA143900.020.93940210-11
AGTACTT76800.020.56595612-13
GTGGTAT36600.019.0714341
Produced by FastQC (version 0.11.5)