FastQCFastQC Report
Mon 15 Aug 2016
SRR2921118_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921118_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3546653
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA99300.029.3768311
TCAACGC109150.026.004264
CAACGCA110250.025.6908345
AACGCAG112900.025.0860466
TATCAAC114650.024.9131872
ATCAACG114100.024.8768183
GTACATG160650.024.1245021
GGTATCA75250.024.050561
TACATGG156650.023.8936422
ACATGGG159050.023.233793
CATGGGG81250.022.9962044
ACGCAGA128550.021.7542957
CGCAGAG130800.021.3348928
GTGTAGC29100.019.8443491
GTATAGG10950.019.0288281
GCAGAGT145150.018.4877839
GTAGCCC27850.017.9480063
TATACTG21050.017.5262685
TAGCCCT35300.016.85684
GTATAGA17750.016.7698731
Produced by FastQC (version 0.11.5)