##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921118_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3546653
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.14476014428251	31.0	30.0	34.0	23.0	34.0
2	29.170016914538863	31.0	30.0	34.0	22.0	34.0
3	29.248471728133538	31.0	30.0	34.0	23.0	34.0
4	32.66755276030669	35.0	33.0	37.0	25.0	37.0
5	32.75824897445563	35.0	33.0	37.0	25.0	37.0
6	32.809216746041976	37.0	33.0	37.0	25.0	37.0
7	32.67805928575476	36.0	33.0	37.0	25.0	37.0
8	32.604604397441754	36.0	33.0	37.0	25.0	37.0
9	33.97763468825397	38.0	34.0	39.0	24.0	39.0
10-11	33.90498985945341	38.0	34.0	39.0	23.0	39.0
12-13	33.88339555631747	38.0	34.0	39.0	23.0	39.0
14-15	34.862762723051844	39.0	34.0	41.0	23.0	41.0
16-17	34.76147652448661	39.0	34.0	41.0	22.0	41.0
18-19	34.829857897008814	39.0	34.0	41.0	22.5	41.0
20-21	34.88317591261395	39.0	34.0	41.0	23.0	41.0
22-23	34.8468316466257	39.0	34.0	41.0	23.0	41.0
24-25	34.766548912453516	39.0	34.0	41.0	22.5	41.0
26-27	34.712994476764436	39.0	34.0	41.0	22.5	41.0
28-29	34.56978579522722	39.0	34.0	41.0	21.5	41.0
30-31	34.470199086293476	39.0	34.0	41.0	20.5	41.0
32-33	34.38685205459908	39.0	34.0	41.0	19.5	41.0
34-35	34.289183633132424	39.0	33.5	41.0	19.0	41.0
36-37	34.14436808450108	39.0	33.0	41.0	18.0	41.0
38-39	33.98784107156804	39.0	33.0	41.0	16.0	41.0
40-41	33.89680016624124	38.5	33.0	41.0	16.0	41.0
42-43	33.80047850748297	38.0	33.0	41.0	15.5	41.0
44-45	33.63748384744716	38.0	33.0	40.0	14.5	41.0
46-47	33.41087991974405	38.0	33.0	40.0	11.5	41.0
48-49	33.280923732882805	38.0	32.5	40.0	9.0	41.0
50-51	33.13027648884737	38.0	32.0	40.0	8.5	41.0
52-53	32.93693110095631	38.0	32.0	40.0	7.5	41.0
54-55	32.70746475620818	38.0	32.0	40.0	6.5	41.0
56-57	32.457232776930816	37.5	31.0	40.0	2.0	41.0
58-59	32.24691871463038	37.0	31.0	40.0	2.0	41.0
60-61	31.963011464611846	37.0	31.0	40.0	2.0	41.0
62-63	31.70175204622499	36.0	30.5	40.0	2.0	41.0
64-65	31.398796273557068	36.0	30.0	39.0	2.0	41.0
66-67	31.131600131165918	35.5	30.0	39.0	2.0	40.5
68-69	30.772275438279415	35.0	29.5	39.0	2.0	40.0
70-71	30.458063278251352	35.0	29.0	38.0	2.0	40.0
72-73	30.093416525383226	35.0	29.0	37.5	2.0	40.0
74-75	30.031038136519136	35.0	29.0	37.0	2.0	39.0
76-77	29.772830186657675	35.0	29.0	37.0	2.0	39.0
78-79	29.49336599887274	34.5	29.0	36.0	2.0	39.0
80-81	29.122184211424123	34.0	29.0	36.0	2.0	37.5
82-83	28.843994323662336	34.0	29.0	35.0	2.0	37.0
84-85	28.6378468657633	34.0	29.0	35.0	2.0	37.0
86-87	28.40496969960129	34.0	29.0	35.0	2.0	36.0
88-89	28.229267424808686	34.0	28.0	35.0	2.0	36.0
90-91	28.056025920776573	34.0	28.0	35.0	2.0	36.0
92-93	28.20832824637764	34.0	29.0	35.0	2.0	35.0
94-95	28.17383473940078	34.0	29.0	35.0	2.0	35.0
96-97	28.103489966455697	34.0	29.0	35.0	2.0	35.0
98-99	27.988208460201772	34.0	29.0	35.0	2.0	35.0
100-101	27.883572201734985	34.0	28.5	35.0	2.0	35.0
102-103	26.56009637819093	32.0	25.0	34.0	2.0	35.0
104-105	27.57660602827511	34.0	27.0	35.0	2.0	35.0
106-107	27.74204129921929	34.0	28.0	35.0	2.0	35.0
108-109	27.686136901467385	34.0	28.0	35.0	2.0	35.0
110-111	27.653652612759128	34.0	28.0	35.0	2.0	35.0
112-113	27.571443414396615	34.0	27.0	35.0	2.0	35.0
114-115	27.43696394882725	34.0	27.0	35.0	2.0	35.0
116-117	27.27653410130622	34.0	27.0	35.0	2.0	35.0
118-119	27.158558928657527	34.0	27.0	35.0	2.0	35.0
120-121	26.980088974027062	34.0	27.0	35.0	2.0	35.0
122-123	26.824850358915857	34.0	26.5	35.0	2.0	35.0
124-125	25.782350712065714	33.0	22.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	169162.0
3	23835.0
4	15585.0
5	13447.0
6	15141.0
7	17009.0
8	18324.0
9	19358.0
10	21119.0
11	22583.0
12	23659.0
13	24244.0
14	24348.0
15	22157.0
16	18779.0
17	16726.0
18	15376.0
19	14634.0
20	14524.0
21	15690.0
22	18612.0
23	24470.0
24	32815.0
25	42780.0
26	50440.0
27	57273.0
28	68238.0
29	78354.0
30	91819.0
31	110384.0
32	135454.0
33	171162.0
34	226252.0
35	317497.0
36	522663.0
37	792608.0
38	279849.0
39	283.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.144710166294445	18.950625208979083	13.30657460535579	24.598090019370687
2	15.386630489922856	20.077528844909317	40.23116533236256	24.30467533280527
3	19.265659185859395	24.36206137048299	29.206677958664066	27.165601484993545
4	12.575417025056753	16.528659456563123	37.37289019867737	33.52303331970276
5	13.603346228823465	37.93439796559755	34.646823459809525	13.81543234576946
6	32.247256468778254	36.569936007299916	17.627797883001694	13.555009640920138
7	27.895010071108256	32.484153125963154	21.217455008525828	18.40338179440276
8	25.627582410858274	36.10585766141892	19.882120954322858	18.384438973399952
9	25.858989621687588	16.760385220759783	19.876783270180695	37.503841887371934
10-11	24.65995604676971	26.729299620347525	28.0146926227688	20.596051710113965
12-13	25.157999187340103	24.094393182454553	27.89301425482161	22.854593375383736
14-15	22.61629847235395	25.678682393121804	25.340840982889816	26.364178151634427
16-17	22.614938740462456	28.626572377479164	26.158820083286745	22.599668798771635
18-19	22.52436298002871	27.973202801532988	27.47981729721179	22.02261692122652
20-21	23.535312945024884	27.027410008036885	27.352692356499304	22.084584690438927
22-23	23.03630538918188	26.94045328550005	26.943272979199545	23.07996834611853
24-25	22.667368014722104	27.11091360829053	27.2169617201844	23.00475665680296
26-27	22.7376958373578	27.227812736013913	27.161156097919275	22.873335328709004
28-29	22.67750020761248	27.30795634455973	27.271833888588027	22.74270955923977
30-31	23.1300491889928	27.263114515229216	27.017247094149923	22.589589201628062
32-33	22.696472676093748	27.216943313025837	27.071168067156993	23.015415943723426
34-35	22.796772809123482	27.292896446672454	27.175621467158212	22.734709277045855
36-37	23.03050562519324	27.083010530503866	27.091018625447163	22.79546521885574
38-39	22.847876830645944	27.381744723427094	27.095907301549527	22.674471144377435
40-41	23.043847015342	27.279560954648446	27.01224890063847	22.664343129371083
42-43	22.922531721520276	27.050012455568446	27.051915683344063	22.975540139567215
44-45	22.992422421421868	27.2234685710733	27.002875867930854	22.78123313957398
46-47	22.8748898216337	27.164204818128862	26.934317621602233	23.026587738635204
48-49	22.72666458484154	27.258045983898516	27.156581680737784	22.858707750522157
50-51	22.723539840475965	27.435638783444183	26.957687007214886	22.88313436886497
52-53	22.78598922703724	27.29941962023657	26.819266250873053	23.095324901853136
54-55	22.887972175266498	27.23010248175306	26.848471091799347	23.033454251181098
56-57	22.86130621655499	27.349960306903554	26.775548827020007	23.01318464952145
58-59	22.796925251073855	27.305483670683135	26.98193125406379	22.915659824179222
60-61	23.04930937382409	27.276868411291215	26.8948531851027	22.77896902978199
62-63	22.798004818774327	27.191632983650283	26.98837883066477	23.02198336691061
64-65	22.721569295326756	27.176034235356518	26.98315442030459	23.119242049012144
66-67	22.840123351838365	27.298206199002255	26.835975157072838	23.02569529208654
68-69	22.95332359433785	27.086461358265613	26.940672486943008	23.01954256045353
70-71	22.82074702824494	26.937690596302176	27.072298408658774	23.169263966794105
72-73	22.85951737978375	26.95727077724933	26.996167431840977	23.18704441112594
74-75	22.7599579706412	27.112700628790897	26.965854459035544	23.16148694153236
76-77	22.773441365957034	26.93392485104677	27.076639683887276	23.215994099108915
78-79	22.813419442881937	26.96947396765112	27.078675762586897	23.138430826880054
80-81	22.807303457432635	27.00153017679505	27.046375324989867	23.14479104078244
82-83	22.829018534529975	26.949920777364444	27.097468442407845	23.123592245697736
84-85	22.99093479259366	26.85821808095951	26.917908429873144	23.232938696573687
86-87	22.796539883413715	26.814465564413226	27.0543966429104	23.33459790926266
88-89	22.849842210365974	26.740341772981708	27.12516943794183	23.28464657871049
90-91	22.866084554728914	26.873513023363614	27.06891000065978	23.191492421247688
92-93	22.879440495082	26.781386152907434	27.199452766113907	23.139720585896658
94-95	22.67577717754711	26.87503903386892	27.194401284949148	23.254782503634818
96-97	22.712851790060988	26.714333768208583	27.257768049554915	23.315046392175518
98-99	22.697925153375788	26.66411868608226	27.16348434606163	23.47447181448032
100-101	22.56157967430538	26.7925701893177	27.20808029200888	23.43776984436804
102-103	22.56493154037608	26.739630676971913	27.235663010430155	23.45977477222185
104-105	22.49747388340148	26.78470296294761	27.12273098898998	23.59509216466093
106-107	22.43802364825331	26.7645463996799	27.297284875082937	23.500145076983852
108-109	22.38735092486753	26.783815096711045	27.22723884295575	23.60159513546568
110-111	22.365054834460317	26.928194442349124	27.1452397304262	23.561510992764358
112-113	22.36346198065969	26.935676994741765	27.132869715909674	23.56799130868887
114-115	22.390248400337022	26.917382045651163	27.14351455515665	23.548854998855166
116-117	22.3619932072119	26.872101677247116	27.230618553692448	23.53528656184853
118-119	22.363443707712975	26.730842757393287	27.167204835284313	23.73850869960943
120-121	22.299581075094903	26.895982725304314	27.15739840782031	23.647037791780477
122-123	22.367507286496558	26.847620656121627	27.121204388730817	23.66366766865099
124-125	22.282584923939044	26.860337768376354	27.12953600940945	23.727541298275153
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	572.0
1	435.0
2	263.0
3	203.5
4	158.0
5	136.0
6	148.5
7	181.0
8	218.5
9	272.0
10	305.0
11	345.0
12	374.0
13	413.5
14	519.5
15	624.0
16	722.0
17	857.0
18	1034.0
19	1230.5
20	1531.0
21	1944.5
22	2553.5
23	3494.0
24	4767.5
25	6531.5
26	9358.0
27	13376.0
28	18056.0
29	23818.0
30	30585.5
31	38445.0
32	47451.0
33	56803.0
34	67194.5
35	77671.0
36	88644.0
37	98724.0
38	105374.0
39	111323.5
40	118014.5
41	123235.0
42	127125.0
43	131789.0
44	136590.0
45	141086.5
46	144073.5
47	144288.5
48	144113.0
49	145244.5
50	145943.0
51	140946.0
52	131062.0
53	123731.5
54	117526.0
55	109376.0
56	99825.5
57	90487.5
58	83089.0
59	73720.0
60	61116.5
61	49305.5
62	38917.0
63	29215.5
64	21205.5
65	15551.0
66	11537.0
67	8481.0
68	6069.0
69	4351.0
70	3281.0
71	2475.5
72	1956.5
73	1499.5
74	1081.0
75	800.0
76	608.0
77	436.0
78	289.5
79	191.5
80	135.0
81	110.0
82	80.5
83	53.5
84	37.0
85	34.0
86	30.5
87	20.5
88	15.0
89	12.5
90	11.0
91	13.5
92	13.5
93	11.5
94	10.0
95	7.0
96	5.5
97	6.0
98	8.5
99	11.5
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07437998586272748
2	0.01015041505329109
3	0.021964370351427107
4	0.019821504951287875
5	0.012744410011354367
6	0.008768830782148691
7	0.010432371026993618
8	0.008627852795297425
9	0.006174835824085413
10-11	0.010813011591492035
12-13	0.007725593679449329
14-15	0.008867515372944576
16-17	0.01326602856270405
18-19	0.006696454375435093
20-21	0.014182385477237271
22-23	0.004750958156887634
24-25	0.004215241806852827
26-27	0.0033693738857452364
28-29	0.010784815994121783
30-31	0.0064708895964730685
32-33	0.00274907074359967
34-35	0.008092136445262618
36-37	0.006809236764916106
38-39	0.0015930512514192958
40-41	0.0013815842711423982
42-43	0.0013110952777167656
44-45	0.002015985211973091
46-47	0.003693623255503146
48-49	0.0024107235751566333
50-51	0.004666371364776876
52-53	0.00513159872138605
54-55	0.004736860358202508
56-57	0.001875007225121826
58-59	0.0024530169712120133
60-61	0.005568630480624972
62-63	0.003045124515987327
64-65	0.004060166021316436
66-67	0.0014097798685126512
68-69	0.0024248213738417602
70-71	0.001508464459308537
72-73	0.0021287676014541033
74-75	0.0023825279777863807
76-77	0.001973691815917712
78-79	2.255647789620242E-4
80-81	1.127823894810121E-4
82-83	7.048899342563256E-5
84-85	4.511295579240484E-4
86-87	2.678581750174037E-4
88-89	1.4097798685126513E-5
90-91	7.612811289968317E-4
92-93	0.002509408165952519
94-95	0.001508464459308537
96-97	0.001184215089550627
98-99	0.001508464459308537
100-101	0.0013674864724572717
102-103	0.0031015157107278325
104-105	0.005681412870105984
106-107	0.007330855316265786
108-109	0.013999114094330627
110-111	0.0109539895783433
112-113	0.0034539606778559956
114-115	0.0079229628610411
116-117	0.00274907074359967
118-119	0.0016071490501044223
120-121	0.005469945889829087
122-123	6.766943368860727E-4
124-125	0.08407927135809452
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3546653.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.15610178356603
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.2899156481548	35.957580792552776
2	13.964993147129636	14.287892216827316
3	5.97957626806089	9.176754365745564
4	3.1911395368538673	6.529850358114328
5	1.839993900467742	4.706345762673423
6	1.2082215591239833	3.7084743033387206
7	0.7796000092345099	2.7916908196008725
8	0.5715343396184351	2.3389975080259093
9	0.4015996528794659	1.8489845447052073
>10	1.6922138307332295	14.188100315408962
>50	0.059545798931718665	2.0632881421446756
>100	0.020614515017263964	1.8198635180324825
>500	7.196483868209037E-4	0.27933638578612485
>1k	3.3214540766554965E-4	0.30284096704363006
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	2.8195597370253026E-5
7	0.0	0.0	0.0	0.0	2.8195597370253026E-5
8	0.0	0.0	0.0	0.0	5.639119474050605E-5
9	0.0	0.0	0.0	5.639119474050605E-5	5.639119474050605E-5
10-11	0.0	0.0	0.0	7.048899342563256E-5	8.458679211075907E-5
12-13	0.0	0.0	0.0	8.458679211075907E-5	1.8327138290664464E-4
14-15	0.0	0.0	0.0	1.5507578553639165E-4	2.1146698027689768E-4
16-17	0.0	0.0	0.0	1.9736918159177116E-4	2.255647789620242E-4
18-19	0.0	0.0	0.0	1.9736918159177116E-4	2.537603763322772E-4
20-21	0.0	0.0	0.0	2.537603763322772E-4	2.537603763322772E-4
22-23	0.0	0.0	0.0	2.9605377238765677E-4	2.8195597370253025E-4
24-25	0.0	0.0	0.0	3.947383631835423E-4	2.8195597370253025E-4
26-27	0.0	0.0	0.0	9.727481092737294E-4	3.101515710727833E-4
28-29	0.0	0.0	0.0	0.002861853133080682	3.101515710727833E-4
30-31	0.0	0.0	0.0	0.010164512851976216	3.101515710727833E-4
32-33	0.0	0.0	0.0	0.024191822543677095	3.242493697579098E-4
34-35	0.0	0.0	0.0	0.04294189479489536	3.383471684430363E-4
36-37	0.0	0.0	0.0	0.07463374623905975	3.383471684430363E-4
38-39	0.0	0.0	0.0	0.1253435281094598	3.383471684430363E-4
40-41	0.0	0.0	0.0	0.18368021906851334	3.383471684430363E-4
42-43	0.0	0.0	0.0	0.23988814242611273	3.383471684430363E-4
44-45	0.0	0.0	0.0	0.30693727297257445	3.947383631835423E-4
46-47	0.0	0.0	0.0	0.3838125692025693	4.793251552943014E-4
48-49	0.0	0.0	0.0	0.4642546084999012	5.357163500348074E-4
50-51	0.0	0.0	0.0	0.5525209260674783	5.357163500348074E-4
52-53	0.0	0.0	0.0	0.6438464659497278	5.357163500348074E-4
54-55	0.0	0.0	0.0	0.7460132130208397	5.357163500348074E-4
56-57	0.0	0.0	0.0	0.8633632892758327	5.639119474050605E-4
58-59	0.0	0.0	0.0	0.9916955507065395	5.921075447753135E-4
60-61	0.0	0.0	0.0	1.127175396070605	5.921075447753135E-4
62-63	0.0	0.0	0.0	1.2685481212850538	6.203031421455666E-4
64-65	0.0	0.0	0.0	1.4240891341780546	6.344009408306931E-4
66-67	0.0	0.0	0.0	1.601326659247465	6.484987395158195E-4
68-69	0.0	0.0	0.0	1.8029956694381999	6.766943368860726E-4
70-71	0.0	0.0	0.0	2.023457045276208	6.907921355711991E-4
72-73	0.0	0.0	0.0	2.2485284012842532	7.612811289968317E-4
74-75	0.0	0.0	0.0	2.4846242358640667	7.753789276819581E-4
76-77	0.0	0.0	0.0	2.748845178820708	7.894767263670846E-4
78-79	0.0	0.0	0.0	3.0423190540489866	8.176723237373377E-4
80-81	0.0	0.0	0.0	3.3553606738522204	8.599657197927173E-4
82-83	0.0	0.0	0.0	3.68904147093048	8.740635184778438E-4
84-85	0.0	0.0	0.0	4.031787152563275	8.740635184778438E-4
86-87	0.0	0.0	0.0	4.398724656739748	9.022591158480968E-4
88-89	0.0	0.0	0.0	4.8024997088804575	9.304547132183498E-4
90-91	0.0	0.0	0.0	5.237684656491627	9.304547132183498E-4
92-93	0.0	0.0	0.0	5.687700488319551	9.586503105886028E-4
94-95	0.0	0.0	0.0	6.1528996493313555	0.0010150415053291088
96-97	0.0	0.0	0.0	6.645321659604139	0.001099628297439868
98-99	0.0	0.0	0.0	7.179191198011195	0.001127823894810121
100-101	0.0	0.0	0.0	7.753831570215637	0.001127823894810121
102-103	0.0	0.0	0.0	8.335619526353437	0.001156019492180374
104-105	0.0	0.0	0.0	8.921989267063905	0.001184215089550627
106-107	0.0	0.0	0.0	9.529703074983654	0.001184215089550627
108-109	0.0	0.0	0.0	10.171547653520093	0.0012547040829762597
110-111	0.0	1.4097798685126513E-5	0.0	10.842602870932115	0.001268801881661386
112-113	0.0	2.8195597370253026E-5	0.0	11.538864952393144	0.001268801881661386
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	9930	0.0	29.376831	1
TCAACGC	10915	0.0	26.00426	4
CAACGCA	11025	0.0	25.690834	5
AACGCAG	11290	0.0	25.086046	6
TATCAAC	11465	0.0	24.913187	2
ATCAACG	11410	0.0	24.876818	3
GTACATG	16065	0.0	24.124502	1
GGTATCA	7525	0.0	24.05056	1
TACATGG	15665	0.0	23.893642	2
ACATGGG	15905	0.0	23.23379	3
CATGGGG	8125	0.0	22.996204	4
ACGCAGA	12855	0.0	21.754295	7
CGCAGAG	13080	0.0	21.334892	8
GTGTAGC	2910	0.0	19.844349	1
GTATAGG	1095	0.0	19.028828	1
GCAGAGT	14515	0.0	18.487783	9
GTAGCCC	2785	0.0	17.948006	3
TATACTG	2105	0.0	17.526268	5
TAGCCCT	3530	0.0	16.8568	4
GTATAGA	1775	0.0	16.769873	1
>>END_MODULE