FastQCFastQC Report
Mon 15 Aug 2016
SRR2921118_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921118_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3546653
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA116300.033.1839751
GGTATCA84050.029.522931
TCAACGC131600.028.7257564
CAACGCA133800.028.252085
ATCAACG134950.028.012273
AACGCAG137700.027.4765346
TATCAAC139150.027.2509292
CATGGGG77000.025.0120854
GTACATG157300.023.8141331
ACGCAGA156100.023.7804727
TACATGG154200.023.4699272
CGCAGAG159250.023.1983498
ACATGGG153000.023.148723
GAGTACT73850.020.9854112-13
GCAGAGT177350.020.5291639
GTACTTT80800.018.92278514-15
AGTACTT84350.018.76103412-13
TATACTG22650.018.6604865
AGAGTAC159800.018.44705810-11
GTGTAGC31050.018.4422321
Produced by FastQC (version 0.11.5)