FastQCFastQC Report
Mon 15 Aug 2016
SRR2921117_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921117_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2860057
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA66400.033.9825441
TCAACGC73300.030.3615884
CAACGCA74800.029.991395
AACGCAG76250.029.5735236
ATCAACG75800.029.4397493
TATCAAC82250.027.3481482
GTACATG115250.026.8108841
TACATGG116300.025.8906442
ACGCAGA87650.025.319827
GGTATCA46550.025.1959841
ACATGGG117600.024.6936073
CATGGGG76900.024.220044
CGCAGAG91650.024.0853378
GCAGAGT102200.020.2600089
CGTATAC2501.26133855E-519.0424163
TATACTG14750.017.3473555
GTGTAGC19950.017.3089141
AGAGTAC93150.017.2811110-11
GTATTAT13150.017.2045211
ATGGGGA44150.016.7127485
Produced by FastQC (version 0.11.5)