##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921117_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2860057 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.68094691819079 31.0 30.0 34.0 18.0 34.0 2 28.68909081182648 31.0 30.0 34.0 16.0 34.0 3 28.85256797329564 31.0 30.0 34.0 18.0 34.0 4 32.22310079834073 35.0 33.0 37.0 20.0 37.0 5 32.30552188295548 35.0 33.0 37.0 22.0 37.0 6 32.238929503852546 35.0 33.0 37.0 22.0 37.0 7 32.16425232084536 35.0 33.0 37.0 19.0 37.0 8 32.12219476744694 35.0 33.0 37.0 19.0 37.0 9 33.4388006952309 38.0 33.0 39.0 18.0 39.0 10-11 33.36265413591407 38.0 33.0 39.0 18.0 39.0 12-13 33.31171983635291 38.0 33.0 39.0 17.0 39.0 14-15 34.2545879330377 39.0 33.0 41.0 17.0 41.0 16-17 34.133348915773354 39.0 33.0 41.0 17.0 41.0 18-19 34.17926478388368 39.0 33.5 41.0 17.5 41.0 20-21 34.21748692421165 39.0 34.0 41.0 18.0 41.0 22-23 34.17044135833656 39.0 33.5 41.0 18.0 41.0 24-25 34.08918738332838 39.0 33.0 41.0 17.5 41.0 26-27 34.02896043680248 39.0 33.0 41.0 16.5 41.0 28-29 33.87773110815624 39.0 33.0 41.0 16.0 41.0 30-31 33.772563623731976 39.0 33.0 41.0 15.0 41.0 32-33 33.682821356357586 38.5 33.0 41.0 14.0 41.0 34-35 33.57611963677647 38.0 33.0 41.0 9.5 41.0 36-37 33.418798821142374 38.0 32.5 41.0 9.0 41.0 38-39 33.25099604658229 38.0 32.0 40.5 9.0 41.0 40-41 33.13655916647815 38.0 32.0 40.0 8.0 41.0 42-43 33.021173179415655 38.0 32.0 40.0 7.5 41.0 44-45 32.83274564108338 38.0 32.0 40.0 2.0 41.0 46-47 32.58146341139354 38.0 31.0 40.0 2.0 41.0 48-49 32.43255361693841 38.0 31.0 40.0 2.0 41.0 50-51 32.25440209758057 38.0 31.0 40.0 2.0 41.0 52-53 32.048737315375185 37.5 31.0 40.0 2.0 41.0 54-55 31.799504345542765 37.0 30.0 40.0 2.0 41.0 56-57 31.53084781177438 37.0 30.0 40.0 2.0 41.0 58-59 31.306098444891134 36.0 30.0 40.0 2.0 41.0 60-61 31.002403973067672 36.0 29.5 39.5 2.0 41.0 62-63 30.72594776957242 35.5 29.0 39.0 2.0 41.0 64-65 30.413426026124654 35.0 29.0 39.0 2.0 40.5 66-67 30.141237045275673 35.0 29.0 39.0 2.0 40.0 68-69 29.790185999789514 35.0 28.0 38.0 2.0 40.0 70-71 29.478197112854744 34.5 28.0 37.5 2.0 40.0 72-73 29.13264927936751 34.0 28.0 37.0 2.0 39.0 74-75 29.08171078408577 34.0 28.0 36.5 2.0 39.0 76-77 28.842061014867888 34.0 28.0 36.0 2.0 39.0 78-79 28.58180134172151 34.0 28.0 36.0 2.0 38.5 80-81 28.242039931372 34.0 27.0 35.0 2.0 37.0 82-83 27.98437059121549 34.0 27.0 35.0 2.0 37.0 84-85 27.798873239239636 34.0 27.0 35.0 2.0 36.0 86-87 27.588576031876286 34.0 26.0 35.0 2.0 36.0 88-89 27.435135558487122 34.0 26.0 35.0 2.0 36.0 90-91 27.275104657005087 34.0 26.0 35.0 2.0 35.0 92-93 27.44450757449939 34.0 27.0 35.0 2.0 35.0 94-95 27.42945402836377 34.0 27.0 35.0 2.0 35.0 96-97 27.36866065956028 34.0 27.0 35.0 2.0 35.0 98-99 27.260419285349908 34.0 26.5 35.0 2.0 35.0 100-101 27.155659310286474 34.0 26.0 35.0 2.0 35.0 102-103 25.841700882185215 31.5 24.0 34.0 2.0 35.0 104-105 26.85050472770298 33.5 25.0 35.0 2.0 35.0 106-107 27.02944679074578 34.0 25.5 35.0 2.0 35.0 108-109 26.97685028655023 34.0 25.0 35.0 2.0 35.0 110-111 26.952265811485574 34.0 25.0 35.0 2.0 35.0 112-113 26.86734792348544 34.0 25.0 35.0 2.0 35.0 114-115 26.743734303197453 34.0 25.0 35.0 2.0 35.0 116-117 26.58805453877318 34.0 25.0 35.0 2.0 35.0 118-119 26.476014289225702 34.0 25.0 35.0 2.0 35.0 120-121 26.305098115177426 34.0 25.0 35.0 2.0 35.0 122-123 26.151563587718705 34.0 24.5 35.0 2.0 35.0 124-125 25.100091186993826 32.5 20.0 34.5 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 167828.0 3 23277.0 4 14851.0 5 12579.0 6 13827.0 7 15396.0 8 16684.0 9 17897.0 10 19192.0 11 19989.0 12 20815.0 13 20849.0 14 20825.0 15 18614.0 16 15709.0 17 14070.0 18 12898.0 19 12392.0 20 12129.0 21 13094.0 22 15522.0 23 20585.0 24 27862.0 25 36002.0 26 41912.0 27 47977.0 28 56395.0 29 65389.0 30 76928.0 31 92066.0 32 112286.0 33 141961.0 34 187933.0 35 266243.0 36 430621.0 37 583079.0 38 174257.0 39 124.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.018589592641284 18.784484378395078 13.25883127239112 24.938094756572525 2 15.807612889915395 19.481179331795907 39.473661205243104 25.237546573045595 3 19.52558675660477 23.663203527394096 28.57589996390912 28.235309752092014 4 12.974636842359264 15.751414057412793 36.089395985734576 35.18455311449336 5 14.307020313761612 37.081295960012405 34.0895000047208 14.522183721505186 6 33.60071697045714 35.28201688852865 17.02738397747264 14.089882163541576 7 29.028294325888982 31.435738772975412 20.67182414409979 18.864142757035818 8 26.43094693858426 35.311453065332046 19.395580936955497 18.862019059128198 9 26.275270701633595 16.28461723783805 19.172427683230396 38.267684377297954 10-11 25.545126131610758 26.042651965153606 27.21455469374781 21.19766720948782 12-13 25.825454523838538 23.401886858098557 27.310401112512317 23.462257505550586 14-15 23.14386575678777 24.893292157880577 24.68499047746263 27.277851607869025 16-17 23.481380861519924 27.80324781264747 25.40406203004988 23.311309295782724 18-19 23.428208974090225 27.07192800782834 26.7861804038755 22.713682614205943 20-21 24.28285454863675 26.24255001523786 26.562990935789948 22.911604500335443 22-23 23.83336894035259 26.01723892566338 26.411130649321677 23.73826148466235 24-25 23.54631667355492 26.326005912008004 26.47751248102234 23.650164933414732 26-27 23.45986320773354 26.42496023541613 26.459733485503456 23.655443071346877 28-29 23.530312710137036 26.54787618878773 26.441817633135305 23.479993467939927 30-31 23.698390039346435 26.435034673991055 26.3881091769593 23.478466109703213 32-33 23.395545578752206 26.378568987234996 26.387013139135323 23.83887229487748 34-35 23.61652518962679 26.466207058606322 26.44835633471663 23.468911417050258 36-37 23.739151566859917 26.2880175952669 26.383232046323947 23.58959879154924 38-39 23.6298607994621 26.459378383401038 26.393959043157363 23.516801773979502 40-41 23.773994587450435 26.356319186579114 26.384798008405536 23.48488821756491 42-43 23.730482681264213 26.26138801632434 26.354341781747287 23.653787520664164 44-45 23.70077064860462 26.336843073244747 26.333066840886175 23.629319437264456 46-47 23.70347344760265 26.376606917733746 26.233911591486464 23.686008043177136 48-49 23.634292103936318 26.505761225070646 26.22035733092026 23.63958934007277 50-51 23.521766540777232 26.646227845073827 26.2232435416832 23.608762072465737 52-53 23.67674478982275 26.464806991135205 26.118641949084015 23.739806269958034 54-55 23.63959894675357 26.421303117098226 26.146068097371273 23.79302983877693 56-57 23.5610016288453 26.511104976693193 26.126717066407135 23.801176328054375 58-59 23.565148418333244 26.42832180088382 26.217795374677422 23.78873440610551 60-61 23.677335075114303 26.500765059575343 26.198045531534582 23.62385433377577 62-63 23.54123517318918 26.46814426467043 26.307094159241352 23.68352640289904 64-65 23.500366701253427 26.37292687041678 26.243414456333703 23.883291971996087 66-67 23.54534950824739 26.43126783310149 26.14415500840814 23.879227650242978 68-69 23.677948998387404 26.28392147099918 26.200686856028767 23.83744267458465 70-71 23.49230591448351 26.261131253733584 26.276078693814014 23.970484137968892 72-73 23.670111311383412 26.239171746803752 26.22275563418817 23.867961307624665 74-75 23.548247687406118 26.32101768116834 26.283167924945243 23.847566706480297 76-77 23.52714417657504 26.177584483057935 26.345801472134667 23.949469868232356 78-79 23.53109314596089 26.237817711513827 26.323760460286707 23.907328682238575 80-81 23.668521643297645 26.255290984016387 26.232529176022553 23.84365819666342 82-83 23.61920948736834 26.287217153653135 26.271553121958647 23.822020237019874 84-85 23.620949718326408 26.238772915699315 26.2103992660263 23.929878099947974 86-87 23.5340383790359 26.24315090884743 26.275353131814782 23.94745758030189 88-89 23.57531173783166 26.139999730774456 26.244683014018435 24.040005517375448 90-91 23.56675404340306 26.247146445018565 26.196989610944506 23.989109900633878 92-93 23.529111475603578 26.14130683719413 26.45045138285497 23.879130304347324 94-95 23.3370582464051 26.196109680275185 26.488502535994478 23.978329537325237 96-97 23.480217522117766 26.078685860398014 26.468576739203176 23.97251987828104 98-99 23.405839895357268 26.070159722826823 26.30426672289011 24.219733658925804 100-101 23.329620650789952 26.08762921472656 26.455266953879462 24.127483180604028 102-103 23.204217244822196 26.004909489482674 26.577607504130725 24.213265761564408 104-105 23.150779507569194 26.15532737080077 26.33283267734053 24.361060444289507 106-107 23.123849705519365 26.20492117548885 26.368670135011595 24.30255898398019 108-109 23.161890954156377 26.162963102838848 26.348176263201662 24.32696967980311 110-111 23.091129581315233 26.286488881335757 26.318642142767473 24.303739394581537 112-113 23.14968614153832 26.263305439659717 26.352327430294224 24.234680988507737 114-115 23.097023699731718 26.35519263735678 26.331957092042657 24.215826570868852 116-117 22.98747688804724 26.292084033801494 26.38000386015066 24.34043521800061 118-119 23.06604769750184 26.216633147134427 26.28956959801736 24.427749557346374 120-121 23.050496729265415 26.26574001681173 26.323067035722826 24.360696218200026 122-123 23.015238937050576 26.292078927512254 26.296379563656757 24.396302571780414 124-125 22.985591225854225 26.35134201185337 26.235915747400373 24.427151014892033 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 450.0 1 347.5 2 221.5 3 165.0 4 117.5 5 113.5 6 118.5 7 149.0 8 210.0 9 247.5 10 282.0 11 314.0 12 356.0 13 407.5 14 468.5 15 544.0 16 611.5 17 690.0 18 817.5 19 995.5 20 1222.5 21 1484.0 22 1905.0 23 2515.5 24 3326.0 25 4521.0 26 6309.0 27 8918.0 28 12012.5 29 15550.0 30 20094.5 31 25614.0 32 32057.5 33 37929.5 34 43287.0 35 49059.0 36 55591.0 37 62412.0 38 68236.0 39 73591.5 40 80091.0 41 86385.5 42 90927.0 43 96357.5 44 102760.0 45 108145.5 46 111883.0 47 113850.5 48 115918.5 49 117860.5 50 118509.5 51 117005.0 52 113487.5 53 110771.5 54 107763.5 55 102079.5 56 94900.5 57 88248.0 58 81893.0 59 73430.0 60 62281.5 61 51717.0 62 42759.5 63 33438.0 64 24899.5 65 18989.0 66 14501.0 67 10931.5 68 8180.0 69 6110.0 70 4872.0 71 3844.0 72 2930.0 73 2383.0 74 1846.5 75 1392.0 76 1061.0 77 743.5 78 529.5 79 350.5 80 236.0 81 175.0 82 127.5 83 91.5 84 68.0 85 51.0 86 37.5 87 27.0 88 24.5 89 20.0 90 17.5 91 17.5 92 13.5 93 9.5 94 9.0 95 8.0 96 6.5 97 9.0 98 9.5 99 10.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07202653653406207 2 0.010559230113245995 3 0.021293281917108643 4 0.019405207658448765 5 0.012936805105632512 6 0.008601227178339452 7 0.009125692250189419 8 0.0076921543871328445 9 0.006083794833459613 10-11 0.010699087465739318 12-13 0.007814529570564503 14-15 0.009178138757374416 16-17 0.013234001979680823 18-19 0.006660706412494576 20-21 0.014108110432764102 22-23 0.004685221308526368 24-25 0.004020898884183078 26-27 0.003496433812333111 28-29 0.01104873084697263 30-31 0.007150207146221212 32-33 0.003129308262038134 34-35 0.008006833430242824 36-37 0.006887974610296228 38-39 0.0016258417227348965 40-41 0.001293680510563251 42-43 0.0011538231580699265 44-45 0.0022202354708315253 46-47 0.004108309729491406 48-49 0.002622325359249833 50-51 0.004895007337266355 52-53 0.005227168549438 54-55 0.004807596491958027 56-57 0.0019405207658448763 58-59 0.0029195222332981476 60-61 0.005209686380376335 62-63 0.002709736204558161 64-65 0.0034789516432714453 66-67 0.00110137665088493 68-69 0.0024125393305098465 70-71 0.0012412340033782542 72-73 0.002167788963646529 74-75 0.0022726819780165223 76-77 0.002115342456461532 78-79 4.7201856466496994E-4 80-81 1.0489301436999331E-4 82-83 5.244650718499666E-5 84-85 3.1467904310998E-4 86-87 2.0978602873998663E-4 88-89 5.244650718499666E-5 90-91 9.79001467453271E-4 92-93 0.0023775749923865153 94-95 0.0012237518343165888 96-97 9.090727912066088E-4 98-99 0.0013985735249332444 100-101 0.00141605569399491 102-103 0.00298945090954481 104-105 0.004982418182574683 106-107 0.006695670750617908 108-109 0.013251484148742491 110-111 0.01104873084697263 112-113 0.003129308262038134 114-115 0.0074998505274545225 116-117 0.0022726819780165223 118-119 0.0011538231580699265 120-121 0.005209686380376335 122-123 5.244650718499666E-4 124-125 0.08351232160757635 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2860057.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.57948877489525 #Duplication Level Percentage of deduplicated Percentage of total 1 75.55322752879373 45.014226714588716 2 13.141848839867862 15.659692708725547 3 4.864191806696963 8.694181834381174 4 2.2700835103001893 5.410016600800197 5 1.2727988738987124 3.7916353110073824 6 0.7532650049803248 2.6927486345248 7 0.49944597054930706 2.0829714917206372 8 0.3290028623627506 1.5681457876039928 9 0.24907619194356265 1.335584896379563 >10 1.0099333451480066 10.089993133134561 >50 0.039951227357638334 1.5909855732193854 >100 0.016233130432235652 1.626646401389563 >500 7.651374808109634E-4 0.28673908466883663 >1k 1.7657018782640298E-4 0.15643182785569887 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.496433812333111E-5 2 0.0 0.0 0.0 3.496433812333111E-5 3.496433812333111E-5 3 0.0 0.0 0.0 3.496433812333111E-5 3.496433812333111E-5 4 0.0 0.0 0.0 3.496433812333111E-5 3.496433812333111E-5 5 0.0 0.0 0.0 3.496433812333111E-5 3.496433812333111E-5 6 0.0 0.0 0.0 3.496433812333111E-5 6.992867624666222E-5 7 0.0 0.0 0.0 6.992867624666222E-5 6.992867624666222E-5 8 0.0 0.0 0.0 6.992867624666222E-5 6.992867624666222E-5 9 0.0 0.0 0.0 6.992867624666222E-5 6.992867624666222E-5 10-11 0.0 0.0 0.0 1.0489301436999333E-4 6.992867624666222E-5 12-13 6.992867624666222E-5 0.0 0.0 1.0489301436999333E-4 2.272681978016522E-4 14-15 1.3985735249332445E-4 0.0 0.0 1.0489301436999333E-4 2.971968740483144E-4 16-17 1.5733952155499E-4 0.0 0.0 1.223751834316589E-4 3.846077193566422E-4 18-19 2.272681978016522E-4 0.0 0.0 1.3985735249332445E-4 4.195720574799733E-4 20-21 2.4475036686331773E-4 0.0 0.0 1.7482169061665555E-4 5.244650718499666E-4 22-23 2.4475036686331773E-4 0.0 0.0 2.971968740483144E-4 5.244650718499666E-4 24-25 3.496433812333111E-4 0.0 0.0 4.7201856466496994E-4 5.244650718499666E-4 26-27 3.671255502949766E-4 0.0 0.0 0.00110137665088493 5.943937480966288E-4 28-29 4.020898884183078E-4 0.0 0.0 0.0026747718664348295 5.943937480966288E-4 30-31 4.195720574799733E-4 0.0 0.0 0.009650157322039386 5.943937480966288E-4 32-33 4.195720574799733E-4 0.0 0.0 0.021992568679575268 5.943937480966288E-4 34-35 4.545363956033044E-4 0.0 0.0 0.03898523700751418 6.64322424343291E-4 36-37 5.594294099732978E-4 0.0 0.0 0.06533086578344417 6.64322424343291E-4 38-39 5.594294099732978E-4 0.0 0.0 0.10970061086195135 7.342511005899533E-4 40-41 5.594294099732978E-4 0.0 0.0 0.16277647613316798 7.342511005899533E-4 42-43 5.594294099732978E-4 0.0 0.0 0.2120412285489415 7.342511005899533E-4 44-45 5.943937480966288E-4 0.0 0.0 0.2683338129275046 8.041797768366155E-4 46-47 6.64322424343291E-4 0.0 0.0 0.334905912714327 8.741084530832777E-4 48-49 6.64322424343291E-4 0.0 0.0 0.408243611928014 9.265549602682744E-4 50-51 6.64322424343291E-4 0.0 0.0 0.48436097602250583 9.79001467453271E-4 52-53 6.64322424343291E-4 0.0 0.0 0.5616671276131909 0.0010314479746382677 54-55 6.992867624666222E-4 0.0 0.0 0.6452843422351373 0.0011188588199465956 56-57 7.342511005899533E-4 0.0 0.0 0.7446005446744592 0.0012062696652549232 58-59 7.342511005899533E-4 0.0 0.0 0.8526578316446141 0.0012237518343165888 60-61 7.517332696516188E-4 0.0 0.0 0.9690366310881217 0.00125871617243992 62-63 7.692154387132844E-4 0.0 0.0 1.0879853093836942 0.0013636091868099132 64-65 7.692154387132844E-4 0.0 0.0 1.2138744087967477 0.0013636091868099132 66-67 9.090727912066088E-4 0.0 0.0 1.3589764120085719 0.0013810913558715788 68-69 9.090727912066088E-4 0.0 0.0 1.5220326028467266 0.0013985735249332444 70-71 9.440371293299399E-4 0.0 0.0 1.7008052636713185 0.0014335378630565753 72-73 9.440371293299399E-4 0.0 0.0 1.8869728820089948 0.0015908773846115653 74-75 9.440371293299399E-4 0.0 0.0 2.078052989852999 0.0017132525680432244 76-77 9.440371293299399E-4 0.0 0.0 2.2899019145422628 0.0017132525680432244 78-79 9.440371293299399E-4 0.0 0.0 2.529040505136786 0.0017132525680432244 80-81 0.0010664123127615988 0.0 0.0 2.7860283903432697 0.00173073473710489 82-83 0.0010838944818232644 0.0 0.0 3.056984528630024 0.0018531099205365488 84-85 0.0010838944818232644 0.0 0.0 3.3364894475879328 0.0018705920895982142 86-87 0.0010838944818232644 0.0 0.0 3.63602893229051 0.0019754851039682077 88-89 0.0010838944818232644 0.0 0.0 3.9715117565838725 0.002027931611153204 90-91 0.0011363409890082611 0.0 0.0 4.329266864261796 0.002027931611153204 92-93 0.0011538231580699265 0.0 0.0 4.695360966582134 0.0021503067945848634 94-95 0.0011538231580699265 0.0 3.496433812333111E-5 5.077503700101081 0.00220275330176986 96-97 0.0011887874961932577 0.0 3.496433812333111E-5 5.478369836685074 0.002395057161448181 98-99 0.0011887874961932577 0.0 3.496433812333111E-5 5.905266923001884 0.0024475036686331776 100-101 0.0012412340033782542 0.0 3.496433812333111E-5 6.383159496471574 0.0024475036686331776 102-103 0.001293680510563251 0.0 3.496433812333111E-5 6.862712876002122 0.0025523966830031707 104-105 0.001608359553673231 0.0 3.496433812333111E-5 7.348017889153957 0.002622325359249833 106-107 0.001643323891796562 0.0 3.496433812333111E-5 7.849249158320971 0.002622325359249833 108-109 0.0016608060608582277 0.0 3.496433812333111E-5 8.384221013776997 0.002657289697373164 110-111 0.0016782882299198932 0.0 3.496433812333111E-5 8.957863427197431 0.0026922540354964953 112-113 0.0016957703989815588 0.0 3.496433812333111E-5 9.546348202151215 0.0026922540354964953 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6640 0.0 33.982544 1 TCAACGC 7330 0.0 30.361588 4 CAACGCA 7480 0.0 29.99139 5 AACGCAG 7625 0.0 29.573523 6 ATCAACG 7580 0.0 29.439749 3 TATCAAC 8225 0.0 27.348148 2 GTACATG 11525 0.0 26.810884 1 TACATGG 11630 0.0 25.890644 2 ACGCAGA 8765 0.0 25.31982 7 GGTATCA 4655 0.0 25.195984 1 ACATGGG 11760 0.0 24.693607 3 CATGGGG 7690 0.0 24.22004 4 CGCAGAG 9165 0.0 24.085337 8 GCAGAGT 10220 0.0 20.260008 9 CGTATAC 250 1.26133855E-5 19.042416 3 TATACTG 1475 0.0 17.347355 5 GTGTAGC 1995 0.0 17.308914 1 AGAGTAC 9315 0.0 17.28111 10-11 GTATTAT 1315 0.0 17.204521 1 ATGGGGA 4415 0.0 16.712748 5 >>END_MODULE