FastQCFastQC Report
Mon 15 Aug 2016
SRR2921117_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921117_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2860057
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA88000.036.5457731
GGTATCA61850.033.1856081
TCAACGC104500.029.9634134
ATCAACG106400.029.6520483
CAACGCA106550.029.3846865
TATCAAC108000.029.1560042
AACGCAG109350.028.6152446
ACGCAGA126600.024.5748487
CGCAGAG128250.024.0727278
GTACATG124300.023.953031
TACATGG123600.022.967112
ACATGGG125100.022.073153
CATGGGG74800.021.9270084
GCAGAGT141850.021.3045549
GAGTACT61750.020.47234712-13
AGAGTAC127400.019.65886110-11
AGTACTT68600.019.59882212-13
GTACTTT65850.019.06250414-15
CAGAGTA137200.018.14625410-11
GGACCGT6750.017.626126
Produced by FastQC (version 0.11.5)