FastQCFastQC Report
Mon 15 Aug 2016
SRR2921116_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921116_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3319890
Sequences flagged as poor quality0
Sequence length125
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39900.049.838491
GTATCAA62650.044.189921
TCAACGC75500.036.0988924
CAACGCA78950.034.371715
TATCAAC83400.032.8954962
AACGCAG82500.032.8882266
ATCAACG85050.031.9074463
GTACATG132350.031.4444891
TACATGG138600.029.970482
ACATGGG142500.028.231463
ACGCAGA98700.027.4298747
CATGGGG111100.026.2992174
CGCAGAG107650.024.9835388
GCAGAGT116800.022.2112589
CAGAGTA109050.020.40959210-11
AGAGTAC98850.019.98710410-11
ATGGGGG62450.018.7725055
ATGGGGA49100.018.5437855
GAGTACA103500.016.7970811
GTGGTAT24800.016.5648961
Produced by FastQC (version 0.11.5)