FastQCFastQC Report
Mon 15 Aug 2016
SRR2921116_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921116_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3319890
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35020.10548542270978857No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34630.10431068499257505No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48200.056.5329781
GTATCAA76400.047.762911
TCAACGC94850.037.2654044
CAACGCA97700.036.1265345
TATCAAC102100.035.2598152
ATCAACG101400.035.210923
AACGCAG103350.034.24666
GTACATG129250.030.677771
ACGCAGA122600.028.433137
TACATGG135700.028.374062
ACATGGG134350.028.0837143
CATGGGG107750.026.9954764
CGCAGAG132950.026.0854118
GCAGAGT142550.023.829019
CAGAGTA136100.022.27115810-11
GAGTACT66850.021.84775712-13
GTGGTAT28200.021.7781431
GTACTTT68100.021.62210314-15
AGAGTAC126900.021.61205510-11
ATGGGGA50700.020.3097365
Produced by FastQC (version 0.11.5)