FastQCFastQC Report
Mon 15 Aug 2016
SRR2921115_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921115_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2468997
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62550.035.7889141
GTACATG115850.030.2182521
TCAACGC75350.029.7732454
TACATGG115300.029.4732252
CAACGCA77450.028.8885445
TATCAAC77100.028.7922292
ATCAACG79000.028.4010963
GGTATCA46250.027.9341871
CATGGGG81350.027.5773074
ACATGGG124550.027.4754583
AACGCAG81100.027.4399686
ACGCAGA92350.023.7106677
CGCAGAG93700.023.1785458
ATGGGGG37600.021.3651125
GCAGAGT101950.020.0768369
TATACTG11050.018.847995
AGAGTAC87100.018.61709810-11
CATGGGT20000.018.1496334
GTATAGG6850.017.383111
CAGAGTA93350.017.17940310-11
Produced by FastQC (version 0.11.5)