##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921115_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2468997
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.79763847424683	31.0	28.0	33.0	12.0	34.0
2	27.801542083688233	31.0	28.0	34.0	12.0	34.0
3	27.944756109464695	31.0	28.0	34.0	12.0	34.0
4	31.22741744927191	35.0	32.0	37.0	12.0	37.0
5	31.308293610725325	35.0	32.0	37.0	16.0	37.0
6	31.23683989895492	35.0	32.0	37.0	11.0	37.0
7	31.12608318276612	35.0	32.0	37.0	10.0	37.0
8	31.076947035577604	35.0	32.0	37.0	10.0	37.0
9	32.27975651651258	37.0	32.0	39.0	10.0	39.0
10-11	32.19044190819187	37.0	32.0	39.0	10.0	39.0
12-13	32.12472433137829	37.0	32.0	39.0	9.0	39.0
14-15	32.968490038667525	38.0	32.0	41.0	8.0	41.0
16-17	32.83257918093865	38.0	32.0	40.0	8.0	41.0
18-19	32.87294111738491	38.0	32.0	40.0	8.0	41.0
20-21	32.89297091085976	38.0	32.0	40.0	7.5	41.0
22-23	32.837743221235186	38.0	32.0	40.0	4.0	41.0
24-25	32.73964468162578	38.0	31.5	40.5	2.0	41.0
26-27	32.65515146433957	38.0	31.0	40.0	2.0	41.0
28-29	32.48769885099091	38.0	31.0	40.0	2.0	41.0
30-31	32.372218354254784	38.0	31.0	40.0	2.0	41.0
32-33	32.25967832281692	38.0	30.0	40.0	2.0	41.0
34-35	32.137470802921186	38.0	30.0	40.0	2.0	41.0
36-37	31.956700433414866	38.0	30.0	40.0	2.0	41.0
38-39	31.7798982744815	38.0	30.0	40.0	2.0	41.0
40-41	31.645239544641	38.0	30.0	40.0	2.0	41.0
42-43	31.500525314530556	38.0	30.0	40.0	2.0	41.0
44-45	31.299976873199927	37.0	30.0	40.0	2.0	41.0
46-47	31.021343687335385	37.0	29.0	40.0	2.0	41.0
48-49	30.841844076764776	37.0	29.0	40.0	2.0	41.0
50-51	30.63896614698195	37.0	28.5	40.0	2.0	41.0
52-53	30.402136981130397	36.0	28.0	40.0	2.0	41.0
54-55	30.125962688492535	36.0	28.0	40.0	2.0	41.0
56-57	29.832773186844697	35.5	27.5	39.5	2.0	41.0
58-59	29.585989776415282	35.0	27.0	39.0	2.0	41.0
60-61	29.24826802138682	35.0	26.5	39.0	2.0	40.5
62-63	28.95617430883877	35.0	26.0	38.5	2.0	40.0
64-65	28.63379785394636	34.0	26.0	38.0	2.0	40.0
66-67	28.354936235240466	34.0	26.0	37.5	2.0	40.0
68-69	27.999653503021673	34.0	25.5	37.0	2.0	40.0
70-71	27.694983428493433	34.0	25.0	36.5	2.0	39.0
72-73	27.363180271178944	34.0	25.0	36.0	2.0	39.0
74-75	27.305666430538395	34.0	25.0	36.0	2.0	39.0
76-77	27.089937533338436	34.0	25.0	35.0	2.0	37.5
78-79	26.85895385859116	33.5	24.5	35.0	2.0	37.0
80-81	26.546842300739936	33.0	24.0	35.0	2.0	37.0
82-83	26.323851345303375	33.0	23.0	35.0	2.0	36.0
84-85	26.155393870466426	33.0	23.0	35.0	2.0	36.0
86-87	25.971464323366938	33.0	23.0	35.0	2.0	35.5
88-89	25.829519031412353	33.0	21.5	35.0	2.0	35.0
90-91	25.688502659176986	33.0	20.0	35.0	2.0	35.0
92-93	25.8700423694318	33.0	23.0	35.0	2.0	35.0
94-95	25.871736377160442	33.0	23.0	35.0	2.0	35.0
96-97	25.81777438368698	33.0	22.5	35.0	2.0	35.0
98-99	25.716608809164207	33.0	20.0	35.0	2.0	35.0
100-101	25.600626489218094	33.0	20.0	35.0	2.0	35.0
102-103	24.33063972941239	31.0	18.5	34.0	2.0	35.0
104-105	25.302018592975205	33.0	19.0	35.0	2.0	35.0
106-107	25.480666643175347	33.0	19.0	35.0	2.0	35.0
108-109	25.43330328064392	33.0	19.0	35.0	2.0	35.0
110-111	25.401265574644277	33.0	19.0	35.0	2.0	35.0
112-113	25.314990054665923	33.0	18.0	35.0	2.0	35.0
114-115	25.20105411225692	33.0	17.5	35.0	2.0	35.0
116-117	25.03504317745222	33.0	12.5	35.0	2.0	35.0
118-119	24.910770648972033	33.0	4.5	35.0	2.0	35.0
120-121	24.71962744385676	33.0	2.0	35.0	2.0	35.0
122-123	24.541439904544234	33.0	2.0	35.0	2.0	35.0
124-125	23.501365736774893	31.5	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	194043.0
3	26358.0
4	16824.0
5	14686.0
6	15742.0
7	17461.0
8	18154.0
9	19256.0
10	20611.0
11	21396.0
12	21535.0
13	21098.0
14	20448.0
15	18286.0
16	15377.0
17	13375.0
18	12581.0
19	11658.0
20	11908.0
21	12811.0
22	15127.0
23	20198.0
24	27479.0
25	34289.0
26	40055.0
27	45919.0
28	52885.0
29	61071.0
30	70764.0
31	83757.0
32	101395.0
33	125126.0
34	163701.0
35	227246.0
36	358342.0
37	420553.0
38	97454.0
39	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.47002528325104	18.74413820105494	13.470484905031766	25.31535161066225
2	16.02720050162469	19.63813676239824	38.33127158456532	26.003391151411755
3	19.365269441671746	23.67551788283792	28.483556085609468	28.475656589880867
4	13.235115993711261	15.714112679605224	34.675773807908236	36.374997518775274
5	14.598830492738783	37.10743004911023	33.22418148263967	15.069557975511316
6	33.94731054003881	34.42167689376924	16.872430650604127	14.75858191558782
7	29.312050279574002	31.659345840577195	20.026037405073648	19.00256647477515
8	27.12583208508756	34.4063791367297	19.16268564702337	19.305103131159374
9	26.80787101075518	15.968766113225675	18.711938358519753	38.51142451749939
10-11	25.787860461781563	25.78800223433545	26.485462439792233	21.938674864090753
12-13	25.955033628679193	23.253357247780855	26.738855569739233	24.05275355380072
14-15	23.634104526808148	24.498616226353573	24.030371122724976	27.836908124113307
16-17	23.963615056992236	27.42618776945968	24.658794955348203	23.951402218199885
18-19	23.940465832924236	26.69589868717161	26.039909788615674	23.323725691288477
20-21	24.774429275534622	25.573600493380578	25.896870091637947	23.75510013944685
22-23	24.510027289619313	25.29631261043689	25.691571608669893	24.502088491273906
24-25	24.229714029035957	25.58979093764056	25.744758454610135	24.435736578713353
26-27	23.930543663256536	25.760920370785907	25.91576552967737	24.392770436280184
28-29	23.910866705120647	25.90213597801642	25.961133398575477	24.22586391828746
30-31	24.238606976982975	25.64606588542463	25.88233006790823	24.232997069684163
32-33	23.97285546949045	25.66178412366157	25.83264747672273	24.532712930125253
34-35	24.173911528935804	25.802787851759863	25.83037189651649	24.192928722787844
36-37	24.282358182959676	25.65645948518541	25.74887093181036	24.312311400044557
38-39	24.157537854371167	25.981821963301677	25.568936987247916	24.29170319507924
40-41	24.194958794608123	25.80032264427355	25.717413677670837	24.28730488344749
42-43	24.13467127046649	25.662977551413036	25.803703966220798	24.398647211899675
44-45	24.075706569605302	25.72302394516543	25.608481488209478	24.59278799701979
46-47	24.10399803638202	25.90620888877368	25.566261575949245	24.42353149889506
48-49	24.07659727518582	25.917914537378202	25.595387330347165	24.410100857088814
50-51	24.037773945657932	26.061491009651068	25.51531604693998	24.38541899775102
52-53	24.174907048252685	25.837578327251308	25.388509849675266	24.599004774820735
54-55	24.194682175418826	25.877992316776133	25.39787689627567	24.52944861152937
56-57	24.029052777389627	25.95245761870913	25.472375964730016	24.546113639171228
58-59	24.158921449381374	25.73421095586249	25.465006685047005	24.641860909709134
60-61	24.343760872849582	25.87360498428639	25.54088312954989	24.24175101331414
62-63	24.033225702449478	25.79056450319409	25.58543532183146	24.590774472524963
64-65	24.009956582221495	25.810825342827094	25.547248502836972	24.63196957211444
66-67	24.107098557104724	25.887301918075206	25.330508661644714	24.67509086317536
68-69	24.095508135773976	25.700143158515505	25.530779080832815	24.673569624877707
70-71	24.083747897645058	25.610951180812147	25.639809514744584	24.66549140679821
72-73	24.261993965045868	25.577188683448433	25.420279876060675	24.740537475445027
74-75	23.897339665880637	25.855691799470055	25.64353580365323	24.60343273099608
76-77	23.895848201303064	25.561877621049238	25.738673138503188	24.803601039144514
78-79	24.086377055547835	25.65329371657792	25.710280604733356	24.55004862314089
80-81	24.009389247323153	25.67994784921554	25.656233742116473	24.654429161344833
82-83	23.86874586496984	25.681749650110884	25.78108150416576	24.668422980753515
84-85	24.041064598126844	25.668087491717255	25.559601747760617	24.73124616239528
86-87	23.882185976225934	25.57674323094476	25.73810456327742	24.80296622955189
88-89	24.007911716359256	25.573811060485507	25.639404511104004	24.778872712051232
90-91	23.912456191440924	25.556109990315846	25.777618377784194	24.75381544045904
92-93	23.818851154609018	25.438033707705888	25.845841783346717	24.897273354338374
94-95	23.772924632233817	25.50403813751539	25.909755524593354	24.81328170565744
96-97	23.828656518982307	25.510956894786712	25.839089612451833	24.821296973779152
98-99	23.762073668744804	25.473261487895194	25.735720440712324	25.028944402647674
100-101	23.496093397973198	25.557304153993492	26.03758705836442	24.909015389668895
102-103	23.441420295695796	25.384514904781337	26.122306390743333	25.05175840877954
104-105	23.37863755207352	25.470145188173515	25.98681985446754	25.164397405285428
106-107	23.232318527162647	25.574395468591586	26.044216390629828	25.149069613615936
108-109	23.261280146638107	25.6952904659912	25.95243375714582	25.090995630224867
110-111	23.178845905352684	25.667622491466236	25.952749660451985	25.200781942729094
112-113	23.222480838215546	25.74707517867692	26.07859779502019	24.95184618808734
114-115	23.237257317192217	25.7957379448957	25.880434654928752	25.086570082983332
116-117	22.975638256614957	25.78582038998636	26.085909598581576	25.15263175481711
118-119	23.131430589628593	25.608666965577825	26.03372430751235	25.22617813728123
120-121	23.108935574711886	25.737549706494985	26.05644085800473	25.0970738607884
122-123	22.98519734344006	25.801904225728617	26.000326045243334	25.21257238558799
124-125	23.002151641254763	25.657713925756216	25.99557997433272	25.344554458656305
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	628.0
1	490.0
2	297.5
3	227.5
4	190.0
5	166.5
6	179.0
7	205.5
8	255.5
9	301.5
10	339.5
11	375.0
12	420.0
13	485.5
14	542.5
15	596.5
16	674.5
17	762.0
18	880.0
19	1008.5
20	1171.0
21	1417.0
22	1809.0
23	2339.5
24	2914.0
25	3701.0
26	5348.5
27	7488.5
28	8865.0
29	10697.0
30	13668.0
31	17414.5
32	21124.0
33	24610.0
34	28982.0
35	33287.5
36	37276.0
37	42115.0
38	47722.5
39	52054.0
40	56857.5
41	62887.5
42	67740.0
43	74593.5
44	81939.5
45	85841.0
46	89447.5
47	94761.0
48	99527.0
49	103261.0
50	107745.0
51	109733.5
52	106804.0
53	105447.0
54	105487.5
55	101389.0
56	94080.0
57	86369.5
58	81269.5
59	75302.5
60	64478.5
61	53110.5
62	43454.5
63	33676.5
64	26411.5
65	21588.0
66	16085.5
67	11601.5
68	8915.0
69	6778.0
70	5106.0
71	3810.0
72	2787.0
73	2203.0
74	1719.0
75	1259.0
76	868.5
77	535.0
78	353.0
79	275.0
80	193.5
81	130.0
82	88.5
83	62.0
84	43.0
85	28.5
86	23.0
87	23.0
88	22.0
89	18.5
90	15.0
91	12.5
92	14.5
93	14.0
94	13.0
95	12.5
96	7.0
97	7.5
98	13.0
99	12.5
100	17.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07091948673894703
2	0.008869998626972816
3	0.01964360426521377
4	0.018550042790655478
5	0.010733103361405462
6	0.00931552367216323
7	0.009761048717353647
8	0.00805995309026297
9	0.005670318756968923
10-11	0.010287578316215046
12-13	0.007958697398174237
14-15	0.008282715612858177
16-17	0.01148239548286207
18-19	0.005224793711778508
20-21	0.01273796606476233
22-23	0.004434999313486408
24-25	0.003746460607283038
26-27	0.0031996798700038923
28-29	0.009923057824695615
30-31	0.0060955926637415924
32-33	0.0026326479943069997
34-35	0.007229656415135377
36-37	0.005953834694817369
38-39	0.001154314889811531
40-41	9.720546440518153E-4
42-43	8.910500903808308E-4
44-45	0.0017618490423439153
46-47	0.003442693531016846
48-49	0.002349132056458554
50-51	0.004029976545131485
52-53	0.004394497036650916
54-55	0.004516003867157392
56-57	0.0017010956270906768
58-59	0.0021871229491165845
60-61	0.004779268666588092
62-63	0.0026326479943069997
64-65	0.0031389264547506536
66-67	0.0015188353813309614
68-69	0.002247876364369823
70-71	0.0015998399350019461
72-73	0.0019036070112681386
74-75	0.002389634333294046
76-77	0.001741597903926169
78-79	3.2401821468393846E-4
80-81	1.0125569208873077E-4
82-83	2.0251138417746154E-5
84-85	3.6452049151943075E-4
86-87	1.6200910734196923E-4
88-89	2.0251138417746154E-5
90-91	6.480364293678769E-4
92-93	0.002673150271142492
94-95	0.0014985842429132154
96-97	0.001073310336140546
98-99	0.0015998399350019461
100-101	0.001437830827659977
102-103	0.003017419624244177
104-105	0.005346300542284984
106-107	0.007452418937730585
108-109	0.013548011601472178
110-111	0.011097623852924893
112-113	0.0034629446694345923
114-115	0.0076144280450725545
116-117	0.002612396855889254
118-119	0.001458081966077723
120-121	0.005245044850196254
122-123	5.872830141146385E-4
124-125	0.08274615157491079
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2468997.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.81097603192496
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.85044351964254	38.78074535175152
2	12.112152921373227	12.55084929408556
3	4.944986148311833	7.686136764251559
4	2.4454955004792325	5.068140350460394
5	1.4649544046594578	3.7950358773837034
6	0.9135121042474662	2.8397972242823286
7	0.6417066354874633	2.3273212977537714
8	0.4396098758663815	1.8221293393528477
9	0.3431706736794811	1.6002006793980423
>10	1.7036654838772802	15.390787041605375
>50	0.09259556791279516	3.2616603910865565
>100	0.04557849579582594	3.9884761119687475
>500	0.0016556311855127172	0.5601425358869015
>1k	4.730374815741435E-4	0.3285777407326709
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	8.100455367098461E-5	0.0	0.0	0.0	0.0
3	8.100455367098461E-5	0.0	0.0	0.0	0.0
4	8.100455367098461E-5	0.0	0.0	8.100455367098461E-5	0.0
5	1.6200910734196923E-4	0.0	0.0	8.100455367098461E-5	0.0
6	1.6200910734196923E-4	0.0	0.0	8.100455367098461E-5	0.0
7	2.8351593784844617E-4	0.0	0.0	8.100455367098461E-5	0.0
8	2.8351593784844617E-4	0.0	0.0	8.100455367098461E-5	0.0
9	2.8351593784844617E-4	0.0	0.0	2.0251138417746154E-4	0.0
10-11	3.2401821468393846E-4	0.0	0.0	2.8351593784844617E-4	0.0
12-13	3.847716299371769E-4	0.0	0.0	2.8351593784844617E-4	0.0
14-15	5.670318756968923E-4	0.0	0.0	3.4426935310168463E-4	0.0
16-17	6.075341525323846E-4	0.0	0.0	4.455250451904154E-4	0.0
18-19	7.492921214566077E-4	0.0	0.0	4.6577618360816155E-4	0.0
20-21	8.100455367098462E-4	0.0	0.0	4.8602732202590766E-4	0.0
22-23	8.505478135453384E-4	0.0	0.0	4.8602732202590766E-4	0.0
24-25	0.0010935614745582922	0.0	0.0	8.707989519630846E-4	0.0
26-27	0.0011340637513937847	0.0	0.0	0.0014378308276599768	0.0
28-29	0.0012353194434825154	0.0	0.0	0.003280684423674877	0.0
30-31	0.0013770774124067385	0.0	0.0	0.010652098807734477	0.0
32-33	0.0013770774124067385	0.0	0.0	0.02432161723971313	0.0
34-35	0.0016200910734196924	0.0	0.0	0.0431754271066348	0.0
36-37	0.0019036070112681384	0.0	0.0	0.0759012667897126	0.0
38-39	0.0020251138417746156	0.0	0.0	0.12580007185103909	0.0
40-41	0.0020251138417746156	0.0	0.0	0.18699901214946799	0.0
42-43	0.0021466206722810925	0.0	0.0	0.24117080741693897	0.0
44-45	0.0025516434406360152	0.0	0.0	0.3070275095514494	0.0
46-47	0.0028959127937377	0.0	0.0	0.38292877634116207	0.0
48-49	0.003037670762661923	0.0	0.0	0.4617259559246123	0.0
50-51	0.003037670762661923	0.0	0.0	0.5442088427000924	0.0
52-53	0.003037670762661923	0.0	0.0	0.6306204503286152	0.0
54-55	0.0030781730394974153	0.0	0.0	0.7281094306716451	4.050227683549231E-5
56-57	0.0032401821468393847	0.0	0.0	0.8412120387347575	4.050227683549231E-5
58-59	0.0032806844236748767	0.0	0.0	0.9687132062128871	4.050227683549231E-5
60-61	0.0032806844236748767	0.0	0.0	1.0990292819310836	4.050227683549231E-5
62-63	0.0032806844236748767	0.0	0.0	1.2294263622029513	4.050227683549231E-5
64-65	0.003321186700510369	0.0	0.0	1.3740397416440766	4.050227683549231E-5
66-67	0.0034831958078523385	0.0	0.0	1.5365146251696538	4.050227683549231E-5
68-69	0.0035236980846878306	0.0	0.0	1.7205772222485487	4.050227683549231E-5
70-71	0.0036249537767765617	0.0	0.0	1.9198889265560064	4.050227683549231E-5
72-73	0.003746460607283038	0.0	0.0	2.1261467713407507	4.050227683549231E-5
74-75	0.003807214022536277	0.0	0.0	2.3404645692157584	4.050227683549231E-5
76-77	0.003827465160954023	0.0	0.0	2.5820606505394696	4.050227683549231E-5
78-79	0.003908469714625008	0.0	0.0	2.851238782388152	4.050227683549231E-5
80-81	0.004313492482979931	0.0	0.0	3.133742163315711	4.050227683549231E-5
82-83	0.0043337436213976765	0.0	0.0	3.4315149026102505	4.050227683549231E-5
84-85	0.0045565061439928846	0.0	0.0	3.740972548771829	4.050227683549231E-5
86-87	0.004779268666588093	0.0	0.0	4.070316812859635	4.050227683549231E-5
88-89	0.004799519805005838	0.0	0.0	4.444355339435406	6.075341525323846E-5
90-91	0.005184291434943015	0.0	0.0	4.838381739629494	8.100455367098461E-5
92-93	0.005305798265449492	0.0	0.0	5.236822887998649	8.100455367098461E-5
94-95	0.005305798265449492	0.0	0.0	5.653328051836434	8.100455367098461E-5
96-97	0.005346300542284984	0.0	0.0	6.098630334504254	8.100455367098461E-5
98-99	0.005832327864310892	0.0	0.0	6.583726104162945	8.100455367098461E-5
100-101	0.006136094940577085	0.0	0.0	7.109992438224914	8.100455367098461E-5
102-103	0.006358857463172292	0.0	0.0	7.643447926425184	8.100455367098461E-5
104-105	0.0070068938925401695	0.0	0.0	8.181358665077358	8.100455367098461E-5
106-107	0.007411916660895092	0.0	0.0	8.733870474528725	8.100455367098461E-5
108-109	0.007472670076148331	0.0	0.0	9.312587257092657	8.100455367098461E-5
110-111	0.0075536746298193146	0.0	0.0	9.930915266401701	8.100455367098461E-5
112-113	0.007594176906654808	0.0	0.0	10.570365213080454	8.100455367098461E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6255	0.0	35.788914	1
GTACATG	11585	0.0	30.218252	1
TCAACGC	7535	0.0	29.773245	4
TACATGG	11530	0.0	29.473225	2
CAACGCA	7745	0.0	28.888544	5
TATCAAC	7710	0.0	28.792229	2
ATCAACG	7900	0.0	28.401096	3
GGTATCA	4625	0.0	27.934187	1
CATGGGG	8135	0.0	27.577307	4
ACATGGG	12455	0.0	27.475458	3
AACGCAG	8110	0.0	27.439968	6
ACGCAGA	9235	0.0	23.710667	7
CGCAGAG	9370	0.0	23.178545	8
ATGGGGG	3760	0.0	21.365112	5
GCAGAGT	10195	0.0	20.076836	9
TATACTG	1105	0.0	18.84799	5
AGAGTAC	8710	0.0	18.617098	10-11
CATGGGT	2000	0.0	18.149633	4
GTATAGG	685	0.0	17.38311	1
CAGAGTA	9335	0.0	17.179403	10-11
>>END_MODULE