FastQCFastQC Report
Mon 15 Aug 2016
SRR2921115_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921115_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2468997
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29870.12098030090761551No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25740.1042528605745572No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA87800.040.487961
GGTATCA60300.035.1143951
TCAACGC110700.031.671074
TATCAAC112900.031.3669742
ATCAACG113550.030.9280413
CAACGCA113850.030.743615
AACGCAG117150.030.4174186
ACGCAGA131200.026.7060997
CGCAGAG134150.026.0306638
GTACATG126450.025.4711821
TACATGG126000.025.077572
CATGGGG81300.024.642334
ACATGGG132050.023.5246723
GAGTACT56800.023.40956712-13
ACCGAGT14150.023.1229938
GCAGAGT145750.023.0206249
AGTACTT60800.022.70118912-13
GTATCGA1852.6419468E-522.5683841
AGAGTAC130200.021.59011710-11
GTACTTT61550.021.21677814-15
Produced by FastQC (version 0.11.5)