FastQCFastQC Report
Mon 15 Aug 2016
SRR2921114_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921114_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208395
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13130.10865652373603003No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12700.10509808464947307No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12590.10418778627849336No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25600.048.6155661
GTATCAA36800.038.1885531
TAGTGCG851.7094621E-435.003745
TATCAAC45550.030.1802562
TCAACGC45850.029.7207324
ATCAACG47550.028.5353783
CAACGCA48100.028.0830385
AACGCAG49400.027.943996
ACCGTAC900.00952034826.449463
CCCGTAC1250.001633347423.800578
GTGGTAT15950.023.5206281
ACGCAGA58650.023.3338957
GTACATG55950.023.2020021
CGCAGAG59100.022.9548668
TACATGG56100.022.489112
GAGTACT22600.021.85500712-13
CATGGGG39250.021.2251974
ACATGGG59200.021.110423
TGGTATC17600.020.9642012
GCAGAGT62950.020.6057369
Produced by FastQC (version 0.11.5)