##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921114_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1208395
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.15481940921636	31.0	28.0	33.0	16.0	34.0
2	28.21373060961027	31.0	28.0	34.0	16.0	34.0
3	28.310858618249828	31.0	28.0	34.0	16.0	34.0
4	31.57930395276379	35.0	32.0	37.0	16.0	37.0
5	31.66907178530199	35.0	32.0	37.0	17.0	37.0
6	31.661493965135573	35.0	32.0	37.0	17.0	37.0
7	31.580824978587298	35.0	32.0	37.0	16.0	37.0
8	31.50017750818234	35.0	32.0	37.0	13.0	37.0
9	32.721596828851496	37.0	32.0	39.0	11.0	39.0
10-11	32.63530012951063	37.0	32.0	39.0	11.0	39.0
12-13	32.60066079386293	37.0	32.0	39.0	10.5	39.0
14-15	33.472772975724	38.0	32.0	41.0	10.0	41.0
16-17	33.3442313978459	38.0	32.0	41.0	10.0	41.0
18-19	33.41083337815863	38.5	32.0	41.0	10.0	41.0
20-21	33.44220060493464	39.0	32.0	41.0	10.0	41.0
22-23	33.40949358446534	39.0	32.0	41.0	10.0	41.0
24-25	33.30768871105889	38.0	32.0	41.0	10.0	41.0
26-27	33.215428729844135	38.0	32.0	41.0	8.5	41.0
28-29	33.04156298230298	38.0	32.0	41.0	8.0	41.0
30-31	32.910645111904635	38.0	31.5	40.5	7.5	41.0
32-33	32.75964150795063	38.0	31.0	40.0	2.0	41.0
34-35	32.61040429660831	38.0	31.0	40.0	2.0	41.0
36-37	32.443903276660365	38.0	31.0	40.0	2.0	41.0
38-39	32.261673542177846	38.0	30.0	40.0	2.0	41.0
40-41	32.110359195461754	38.0	30.0	40.0	2.0	41.0
42-43	31.994701235936923	38.0	30.0	40.0	2.0	41.0
44-45	31.78679074309311	38.0	30.0	40.0	2.0	41.0
46-47	31.49447366134418	37.0	30.0	40.0	2.0	41.0
48-49	31.300619002892265	37.0	30.0	40.0	2.0	41.0
50-51	31.102991571464628	37.0	29.5	40.0	2.0	41.0
52-53	30.866248619035993	36.5	29.0	40.0	2.0	41.0
54-55	30.584378866182	36.0	29.0	40.0	2.0	41.0
56-57	30.294822884901045	36.0	28.0	40.0	2.0	41.0
58-59	30.046242743473783	35.0	28.0	39.0	2.0	41.0
60-61	29.72032034227219	35.0	28.0	39.0	2.0	41.0
62-63	29.42616818176176	35.0	27.5	39.0	2.0	40.5
64-65	29.082291800280537	35.0	27.0	38.0	2.0	40.0
66-67	28.795050045721805	34.0	26.0	38.0	2.0	40.0
68-69	28.43318575465804	34.0	26.0	37.0	2.0	40.0
70-71	28.127471563520206	34.0	26.0	36.5	2.0	39.0
72-73	27.789574187248377	34.0	26.0	36.0	2.0	39.0
74-75	27.73469105714605	34.0	26.0	36.0	2.0	39.0
76-77	27.511471414562294	34.0	26.0	35.5	2.0	37.5
78-79	27.27350162819277	34.0	25.5	35.0	2.0	37.0
80-81	26.954862855274975	34.0	25.0	35.0	2.0	37.0
82-83	26.732519995531263	33.0	25.0	35.0	2.0	36.0
84-85	26.58479139685285	33.0	24.5	35.0	2.0	36.0
86-87	26.39837925512767	33.0	24.0	35.0	2.0	35.5
88-89	26.257560234856978	33.0	24.0	35.0	2.0	35.0
90-91	26.10821296016617	33.0	23.5	35.0	2.0	35.0
92-93	26.29778176837872	33.5	24.0	35.0	2.0	35.0
94-95	26.283087070039183	34.0	24.0	35.0	2.0	35.0
96-97	26.223994223743063	34.0	24.0	35.0	2.0	35.0
98-99	26.115811882703916	33.5	24.0	35.0	2.0	35.0
100-101	26.003965176949592	33.0	23.0	35.0	2.0	35.0
102-103	24.759372556159203	31.5	19.5	34.0	2.0	35.0
104-105	25.704448876402168	33.0	21.5	35.0	2.0	35.0
106-107	25.86299306104378	33.0	23.0	35.0	2.0	35.0
108-109	25.797331998229055	33.0	20.0	35.0	2.0	35.0
110-111	25.75503208801758	33.5	20.0	35.0	2.0	35.0
112-113	25.65656676831665	33.0	20.0	35.0	2.0	35.0
114-115	25.504508873340257	33.0	19.0	35.0	2.0	35.0
116-117	25.321398218297823	33.0	18.0	35.0	2.0	35.0
118-119	25.183757380657816	33.0	16.0	35.0	2.0	35.0
120-121	24.978731292334047	33.0	4.5	35.0	2.0	35.0
122-123	24.797255450411498	33.0	2.0	35.0	2.0	35.0
124-125	23.75526628296211	32.0	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	83010.0
3	11030.0
4	7505.0
5	7145.0
6	8210.0
7	8515.0
8	8616.0
9	8932.0
10	9664.0
11	10399.0
12	11065.0
13	11383.0
14	11342.0
15	9955.0
16	7262.0
17	6460.0
18	5825.0
19	5729.0
20	5595.0
21	6110.0
22	7320.0
23	9973.0
24	13831.0
25	17011.0
26	18862.0
27	21282.0
28	24759.0
29	28955.0
30	33455.0
31	39857.0
32	48585.0
33	60324.0
34	78992.0
35	111006.0
36	176659.0
37	219502.0
38	54247.0
39	23.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.418062626911755	18.81581606118927	13.295217021163827	24.470904290735152
2	17.435542618881897	20.407538325171586	36.03513757997946	26.121781475967058
3	19.96929176547517	24.029565987526333	28.331615824259504	27.669526422738993
4	14.798864370023344	17.293691128511597	32.98715380667803	34.92029069478703
5	15.802058374405641	36.284580400336026	31.819141143899987	16.094220081358344
6	33.21873422331102	33.85005007158996	16.713813965421636	16.21740173967739
7	28.225740374898717	32.24378927609903	20.017082232243872	19.51338811675838
8	26.46855082791035	33.46732512168093	19.4296369310832	20.634487119325524
9	26.25357110935291	16.99376317958666	19.05317187339652	37.69949383766391
10-11	25.618694136687715	26.30038224689334	25.691692244282127	22.38923137213682
12-13	25.957139357504172	24.667959956566644	25.489659749993375	23.885240935935805
14-15	23.985240157794745	25.79173592843041	23.431725384503654	26.79129852927119
16-17	23.78383500884969	27.87443166041029	24.553511756202955	23.788221574537065
18-19	23.614196192411967	26.722427097367923	26.381293427921648	23.28208328229846
20-21	24.937760091638335	25.496015652674824	26.092219349328104	23.474004906358733
22-23	24.781185603270632	25.28415250800692	25.79762978656493	24.137032102157523
24-25	24.240608572450046	25.80995683319871	25.901984216310698	24.047450378040544
26-27	23.857767618808015	25.90866940898161	26.20605927190586	24.027503700304507
28-29	23.868128666707772	25.992682826906773	26.368224879961033	23.77096362642442
30-31	24.09537496549918	25.801153599941074	26.100084870990713	24.003386563569034
32-33	23.914053652123933	25.67466525869599	26.028037209156057	24.383243880024015
34-35	24.213391178262135	25.87458453613708	25.908185796763682	24.003838488837097
36-37	24.076683297188122	25.76605296359699	25.998193360122585	24.159070379092302
38-39	23.894051014007253	25.92434130492712	26.178029318737412	24.00357836232822
40-41	23.96575074076664	25.855145312739864	26.38548498750183	23.793618958991672
42-43	24.015902302134638	25.788608453394364	26.016268909120775	24.179220335350223
44-45	23.90869518442199	25.960177760123145	25.898855483006034	24.232271572448834
46-47	23.977266379358504	26.085567626728352	25.76392868743754	24.173237306475606
48-49	23.795309344897216	26.219214472508195	25.815237180972556	24.170239001622033
50-51	23.752173968815676	26.28380539611853	25.832149538433786	24.13187109663201
52-53	24.11559756026913	26.07872021715922	25.52287040791837	24.282811814653282
54-55	23.969514862084047	26.089264780669797	25.516654645909032	24.424565711337127
56-57	23.91322338329332	26.330121560750996	25.432505190889852	24.324149865065827
58-59	23.88588665629448	26.17688437220696	25.53568472971631	24.40154424178225
60-61	23.859399685351637	26.18959343161198	25.75568986363858	24.195317019397798
62-63	23.880483996037594	26.13834322141553	25.757081723364866	24.22409105918201
64-65	23.958147991522345	26.121092098087228	25.569101082216868	24.351658828173555
66-67	23.935371270106483	26.369624099723683	25.418722106226603	24.276282523943234
68-69	23.977375985011115	26.24813590629298	25.566386562967057	24.208101545728848
70-71	23.817369834650044	26.154762195391918	25.71839157745951	24.30947639249853
72-73	23.915342645548535	26.125662769152342	25.680768501883104	24.278226083416023
74-75	23.77166090339089	26.222359965225866	25.710471838291184	24.295507293092054
76-77	23.950685736393375	26.113636316615946	25.726959195115747	24.20871875187494
78-79	23.919772689562944	26.267645874702033	25.605401891688253	24.207179544046774
80-81	23.921604974540568	26.216719229588385	25.752052519337663	24.10962327653338
82-83	23.943597866258852	26.08242841324932	25.863129078760732	24.11084464173109
84-85	23.902402289329018	26.137320018636384	25.682871989069746	24.277405702964856
86-87	23.828628316496893	26.07388668071841	25.879702844526314	24.217782158258377
88-89	23.928402520865497	26.043398906565695	25.659873493300406	24.368325079268402
90-91	23.822930166122962	26.08467355521681	25.744549322978994	24.34784695568123
92-93	23.81812847808555	26.093136246818542	25.873170416830284	24.21556485826562
94-95	23.68564241827147	26.061062972561093	25.979548575753146	24.27374603341428
96-97	23.735662622684917	25.972086595276323	25.928433108790284	24.36381767324848
98-99	23.597819057222647	26.180044050840113	25.748597394934443	24.473539497002797
100-101	23.520695674913654	26.28774628521397	25.83312954818453	24.358428491687842
102-103	23.287459806009558	26.40979477530882	25.91846707744432	24.384278341237305
104-105	23.252294790887788	26.639601865211993	25.724747576105788	24.38335576779443
106-107	23.224242022982416	26.65157678254429	25.730293838941005	24.39388735553229
108-109	23.16101022616244	26.756823752539365	25.733917827357416	24.348248193940776
110-111	23.23799001046956	26.96196612498086	25.592192110174505	24.207851754375078
112-113	23.25637978463158	27.033106356778013	25.59345486565129	24.117058992939118
114-115	23.23618708456561	27.113747141124485	25.54443293606671	24.1056328382432
116-117	23.16639205408282	27.12317181258374	25.596154816947468	24.11428131638597
118-119	23.27768162045253	27.04661660233114	25.432552796992002	24.243148980224323
120-121	23.288803776433046	27.102105705697753	25.451943830192924	24.157146687676274
122-123	23.236446361421535	27.116131958053067	25.492765544727504	24.154656135797893
124-125	23.297970758582306	27.24335906085571	25.287871346375717	24.170798834186268
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1019.0
1	731.5
2	351.0
3	225.0
4	178.0
5	158.0
6	163.0
7	205.0
8	250.0
9	270.0
10	292.5
11	336.5
12	384.5
13	417.0
14	465.0
15	556.0
16	628.0
17	686.5
18	806.5
19	939.5
20	1109.0
21	1282.5
22	1483.5
23	1780.5
24	2187.5
25	2678.5
26	3394.0
27	4314.5
28	5278.5
29	6440.0
30	7686.5
31	9342.5
32	11376.5
33	13058.0
34	14628.0
35	16595.0
36	19038.0
37	21551.5
38	23807.5
39	25658.5
40	27634.5
41	29889.0
42	31954.0
43	34946.0
44	38416.0
45	41412.0
46	44302.0
47	46189.5
48	47712.0
49	50020.0
50	51586.5
51	51571.0
52	50931.0
53	50588.0
54	50055.5
55	48193.0
56	45614.5
57	42498.5
58	39584.0
59	35814.5
60	30468.5
61	25636.5
62	21022.0
63	16154.5
64	12287.0
65	9834.5
66	7815.5
67	6021.5
68	4645.5
69	3578.0
70	2657.5
71	2014.0
72	1579.0
73	1218.0
74	905.5
75	711.5
76	547.5
77	358.0
78	236.5
79	143.5
80	91.0
81	71.5
82	46.0
83	37.5
84	30.0
85	18.5
86	13.0
87	11.0
88	9.5
89	10.5
90	11.5
91	9.5
92	7.5
93	4.5
94	1.5
95	2.0
96	4.5
97	4.5
98	3.5
99	5.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0714170449232246
2	0.009599510093967617
3	0.020688599340447453
4	0.0211023713272564
5	0.01324070357788637
6	0.010344299670223727
7	0.010096036478138358
8	0.009268492504520458
9	0.007199632570475713
10-11	0.011958010418778628
12-13	0.00885472051771151
14-15	0.009640887292648512
16-17	0.013282080776567265
18-19	0.005875562212687077
20-21	0.013819984359418898
22-23	0.005379035828516338
24-25	0.004882509444345599
26-27	0.0040135882720468054
28-29	0.01170974722669326
30-31	0.007489272961241978
32-33	0.0030205355037053283
34-35	0.00885472051771151
36-37	0.006165202603453342
38-39	0.001737842344597586
40-41	0.0015309563511931117
42-43	0.0013654475564695318
44-45	0.0020688599340447453
46-47	0.004344605861493965
48-49	0.0028964039076626435
50-51	0.005254904232473653
52-53	0.005379035828516338
54-55	0.005668676219282603
56-57	0.0023171231261301145
58-59	0.0028964039076626435
60-61	0.005130772636430968
62-63	0.003144667099748013
64-65	0.003475684689195172
66-67	0.001324070357788637
68-69	0.0027308951129390636
70-71	0.0015309563511931117
72-73	0.0014895791525122166
74-75	0.00211023713272564
76-77	0.0018619739406402708
78-79	2.896403907662643E-4
80-81	1.6550879472357962E-4
82-83	8.275439736178981E-5
84-85	4.965263841707389E-4
86-87	3.3101758944715923E-4
88-89	4.1377198680894904E-5
90-91	0.0010758071657032675
92-93	0.00211023713272564
94-95	0.0016137107485549014
96-97	0.0012413159604268472
98-99	0.0015309563511931117
100-101	0.0011999387617459521
102-103	0.0030205355037053283
104-105	0.005006641040388284
106-107	0.006620351788943185
108-109	0.014109624750185162
110-111	0.010758071657032676
112-113	0.003889456676004121
114-115	0.007985799345412718
116-117	0.002151614331406535
118-119	0.0013654475564695318
120-121	0.004882509444345599
122-123	9.10298370979688E-4
124-125	0.08258888856706624
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1208395.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.97661711893261
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.935186664571	58.0352380291389
2	10.219711571187693	14.302816873658525
3	3.0756215965290887	6.456647845891088
4	1.2978944258535003	3.6328904519498897
5	0.698789867368375	2.444947549771324
6	0.4257539073808518	1.7875690898207615
7	0.29719997672776655	1.455793428546422
8	0.21542422272253953	1.2059726681279048
9	0.14970377861716372	0.9428187597795644
>10	0.6572288497873033	7.612913370147083
>50	0.01917308704426178	0.9035942962840675
>100	0.007837083370319202	0.843555150944907
>500	1.1874221000221228E-4	0.05600386083331791
>1k	3.562266300066369E-4	0.31923862510624246
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1313	0.10865652373603003	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1270	0.10509808464947307	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1259	0.10418778627849336	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.275439736178981E-5
2	0.0	0.0	0.0	0.0	8.275439736178981E-5
3	0.0	0.0	0.0	0.0	8.275439736178981E-5
4	0.0	0.0	0.0	0.0	8.275439736178981E-5
5	0.0	0.0	0.0	0.0	2.4826319208536945E-4
6	0.0	0.0	0.0	0.0	2.4826319208536945E-4
7	0.0	0.0	0.0	0.0	2.4826319208536945E-4
8	0.0	0.0	0.0	0.0	2.4826319208536945E-4
9	0.0	0.0	0.0	0.0	2.4826319208536945E-4
10-11	0.0	0.0	0.0	0.0	2.4826319208536945E-4
12-13	0.0	0.0	0.0	8.275439736178981E-5	4.965263841707388E-4
14-15	0.0	0.0	0.0	8.275439736178981E-5	7.861667749370033E-4
16-17	0.0	0.0	0.0	8.275439736178981E-5	9.10298370979688E-4
18-19	0.0	0.0	0.0	1.6550879472357962E-4	0.0011171843643841624
20-21	0.0	0.0	0.0	2.482631920853694E-4	0.0011999387617459521
22-23	0.0	0.0	0.0	3.7239478812805415E-4	0.001324070357788637
24-25	0.0	0.0	0.0	8.275439736178981E-4	0.001324070357788637
26-27	0.0	0.0	0.0	0.002606763516896379	0.0014895791525122166
28-29	0.0	0.0	0.0	0.007406518563880188	0.0014895791525122166
30-31	0.0	0.0	0.0	0.020936862532532824	0.0014895791525122166
32-33	0.0	0.0	0.0	0.05230077913265116	0.0015723335498740063
34-35	0.0	0.0	0.0	0.10373263709300352	0.0016550879472357963
36-37	0.0	0.0	0.0	0.17912189308959403	0.0016550879472357963
38-39	0.0	0.0	0.0	0.27408256406224785	0.0016550879472357963
40-41	0.0	0.0	0.0	0.36300216402749097	0.0016550879472357963
42-43	0.0	0.0	0.0	0.45390786952941714	0.0016550879472357963
44-45	0.0	0.0	0.0	0.5780394655721018	0.0016550879472357963
46-47	0.0	0.0	0.0	0.7182667919016547	0.0016964651459166911
48-49	0.0	0.0	0.0	0.8513358628594127	0.001737842344597586
50-51	0.0	0.0	0.0	0.9731917129746482	0.001737842344597586
52-53	0.0	0.0	0.0	1.0965785194410769	0.001737842344597586
54-55	0.0	0.0	0.0	1.2504189441366442	0.001779219543278481
56-57	0.0	0.0	0.0	1.4391403473202056	0.0018619739406402708
58-59	0.0	0.0	0.0	1.6259170221657653	0.0019447283380020605
60-61	0.0	0.0	0.0	1.8112454950574937	0.0020688599340447453
62-63	0.0	0.0	0.0	2.0057183288576996	0.0020688599340447453
64-65	0.0	0.0	0.0	2.231182684469896	0.0020688599340447453
66-67	0.0	0.0	0.0	2.5010033970680117	0.0020688599340447453
68-69	0.0	0.0	0.0	2.786712953959591	0.0020688599340447453
70-71	0.0	0.0	0.0	3.0684502997778047	0.0020688599340447453
72-73	0.0	0.0	0.0	3.358463085332197	0.002234368728768325
74-75	0.0	0.0	0.0	3.680501822665602	0.0023585003248110096
76-77	0.0	0.0	0.0	4.0531034967870605	0.0023998775234919047
78-79	0.0	0.0	0.0	4.450655621713099	0.0023998775234919047
80-81	0.0	0.0	0.0	4.84464930755258	0.0023998775234919047
82-83	0.0	0.0	0.0	5.255318004460462	0.0023998775234919047
84-85	4.1377198680894904E-5	0.0	0.0	5.700453907869529	0.0024412547221727993
86-87	8.275439736178981E-5	0.0	0.0	6.19338047575503	0.002565386318215484
88-89	8.275439736178981E-5	0.0	0.0	6.728429031897683	0.002606763516896379
90-91	8.275439736178981E-5	0.0	0.0	7.276097633638008	0.002648140715577274
92-93	8.275439736178981E-5	0.0	0.0	7.8270350340741235	0.0027308951129390636
94-95	8.275439736178981E-5	0.0	0.0	8.40908808791827	0.0027308951129390636
96-97	8.275439736178981E-5	0.0	0.0	9.022380926766495	0.0027722723116199587
98-99	8.275439736178981E-5	0.0	0.0	9.700966985133173	0.0028964039076626435
100-101	8.275439736178981E-5	0.0	0.0	10.392669615481694	0.0028964039076626435
102-103	8.275439736178981E-5	0.0	0.0	11.085737693386683	0.003020535503705328
104-105	8.275439736178981E-5	0.0	0.0	11.77590936738401	0.0031446670997480127
106-107	8.275439736178981E-5	0.0	0.0	12.521071338428246	0.0031446670997480127
108-109	8.275439736178981E-5	0.0	0.0	13.303803805874734	0.0031446670997480127
110-111	8.275439736178981E-5	0.0	0.0	14.11086606614559	0.003227421497109803
112-113	8.275439736178981E-5	0.0	0.0	14.924879695794836	0.003227421497109803
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2560	0.0	48.615566	1
GTATCAA	3680	0.0	38.188553	1
TAGTGCG	85	1.7094621E-4	35.00374	5
TATCAAC	4555	0.0	30.180256	2
TCAACGC	4585	0.0	29.720732	4
ATCAACG	4755	0.0	28.535378	3
CAACGCA	4810	0.0	28.083038	5
AACGCAG	4940	0.0	27.94399	6
ACCGTAC	90	0.009520348	26.44946	3
CCCGTAC	125	0.0016333474	23.80057	8
GTGGTAT	1595	0.0	23.520628	1
ACGCAGA	5865	0.0	23.333895	7
GTACATG	5595	0.0	23.202002	1
CGCAGAG	5910	0.0	22.954866	8
TACATGG	5610	0.0	22.48911	2
GAGTACT	2260	0.0	21.855007	12-13
CATGGGG	3925	0.0	21.225197	4
ACATGGG	5920	0.0	21.11042	3
TGGTATC	1760	0.0	20.964201	2
GCAGAGT	6295	0.0	20.605736	9
>>END_MODULE