FastQCFastQC Report
Mon 15 Aug 2016
SRR2921114_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921114_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208395
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33090.2738343008701625No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26120.216154485908995No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16260.13455865011027024No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15440.12777278952660348No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35900.059.6517071
GTATCAA52950.040.198412
GTGGTAT19150.033.3301961
TGGTATC20150.031.9562172
TATCAAC68150.031.5826073
TCAACGC68250.031.3109635
ATCAACG69600.031.01034
CAACGCA70400.030.3333766
AACGCAG72150.029.8449787
ACGCAGA81400.025.6496688
CGCAGAG82650.025.2627879
GAGTACT45250.025.04392212-13
GTACTTT46200.024.53047414-15
CAGAGTA82800.023.20540410-11
GCAGAGT85700.021.9342739
GTACATG57150.021.3973751
ACTTTTT56150.020.71375816-17
AGTACTT48750.020.43930812-13
TACATGG59200.020.2432862
AGAGTAC79900.019.99007610-11
Produced by FastQC (version 0.11.5)