FastQCFastQC Report
Mon 15 Aug 2016
SRR2921113_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921113_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3612697
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA88000.028.6854381
GTACATG144950.026.779821
TACATGG146750.025.2241272
TCAACGC98750.024.9494574
CAACGCA98450.024.7222185
ATCAACG100500.024.4561373
TATACCG3202.0008883E-1124.1753735
CATGGGG92550.024.1130814
AACGCAG102550.023.7895346
TATCAAC104300.023.6792352
ACATGGG152900.023.6646423
GGTATCA67850.021.4978241
ACGCAGA113950.021.0962267
CGCAGAG120100.019.7682238
TACACCG5303.6379788E-1217.9648655
GTATATA17750.017.1060641
GCAGAGT129750.017.1056359
ACCGTAT4552.1518645E-917.0008138
TACCGTA3901.1743032E-716.7828547
TATACAG23300.016.6011165
Produced by FastQC (version 0.11.5)