FastQCFastQC Report
Mon 15 Aug 2016
SRR2921113_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921113_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3612697
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA95700.033.7807271
TCAACGC111700.028.2411044
GGTATCA69850.027.8376671
CAACGCA113350.027.7277645
ATCAACG115400.027.4898873
TATCAAC118050.026.9711972
AACGCAG119800.026.2682176
GTACATG156550.024.6890051
TACATGG155300.023.9957032
CATGGGG96800.023.3476434
ACATGGG155400.023.2918953
ACGCAGA138500.022.6788987
CGCAGAG143650.021.7007878
GCAGAGT157500.019.3019949
AGAGTAC140800.018.50701110-11
GTATAGG11200.018.1068361
GAGTACT54550.017.0680712-13
TACCGTA2955.2425563E-516.1326527
ATGGGGA50300.016.0943475
CAGAGTA149650.015.98137610-11
Produced by FastQC (version 0.11.5)