##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921113_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3612697
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.60587644078648	31.0	31.0	34.0	27.0	34.0
2	30.78030651338875	31.0	31.0	34.0	27.0	34.0
3	30.860568157252047	31.0	31.0	34.0	27.0	34.0
4	34.37139677088889	37.0	35.0	37.0	30.0	37.0
5	34.02722536653364	37.0	35.0	37.0	30.0	37.0
6	33.91035699921693	37.0	35.0	37.0	29.0	37.0
7	33.728845236674985	37.0	35.0	37.0	28.0	37.0
8	33.724274136469234	37.0	35.0	37.0	28.0	37.0
9	35.15341281042944	39.0	35.0	39.0	28.0	39.0
10-11	35.10732605031643	39.0	35.0	39.0	28.0	39.0
12-13	34.995023523976684	39.0	35.0	39.0	27.0	39.0
14-15	36.09903238494676	40.0	36.0	41.0	27.0	41.0
16-17	36.049623868262415	40.0	36.0	41.0	27.0	41.0
18-19	36.07430238406376	40.0	36.0	41.0	27.0	41.0
20-21	36.05771090130172	40.0	36.0	41.0	27.0	41.0
22-23	36.02562351617088	40.0	36.0	41.0	27.0	41.0
24-25	35.93543743081692	40.0	36.0	41.0	27.0	41.0
26-27	35.83348202188006	40.0	35.5	41.0	27.0	41.0
28-29	35.741401922165075	39.0	35.0	41.0	27.0	41.0
30-31	35.673804362779386	39.0	35.0	41.0	27.0	41.0
32-33	35.559005640384456	39.0	35.0	41.0	26.0	41.0
34-35	35.45182352685542	39.0	35.0	41.0	25.5	41.0
36-37	35.32673221695592	39.0	35.0	41.0	25.0	41.0
38-39	35.173809760408915	39.0	35.0	41.0	25.0	41.0
40-41	35.04073314202658	39.0	34.5	41.0	24.5	41.0
42-43	34.92557236325106	39.0	34.0	41.0	24.0	41.0
44-45	34.78038457141576	39.0	34.0	41.0	24.0	41.0
46-47	34.5963421510301	39.0	34.0	41.0	23.0	41.0
48-49	34.4269616300509	39.0	33.5	41.0	23.0	41.0
50-51	34.225423554757015	38.0	33.0	40.5	22.0	41.0
52-53	34.05667317242492	38.0	33.0	40.0	21.5	41.0
54-55	33.8584106278495	38.0	33.0	40.0	20.5	41.0
56-57	33.58669451105365	38.0	33.0	40.0	19.0	41.0
58-59	33.36268084480929	37.5	32.5	40.0	18.5	41.0
60-61	33.09002637088026	37.0	32.0	40.0	18.0	41.0
62-63	32.7900816204625	36.5	32.0	40.0	15.5	41.0
64-65	32.492219801439205	36.0	32.0	39.0	13.5	41.0
66-67	32.15659284462549	35.5	31.0	39.0	12.5	41.0
68-69	31.79335922719232	35.0	31.0	39.0	9.0	40.0
70-71	31.430368087885586	35.0	31.0	38.0	8.0	40.0
72-73	31.232180002917488	35.0	31.0	37.0	8.0	39.5
74-75	31.029149690660468	35.0	31.0	37.0	7.0	39.0
76-77	30.785107358851295	35.0	31.0	36.0	7.0	39.0
78-79	30.461015551539475	35.0	31.0	36.0	4.0	38.5
80-81	30.163407421103955	35.0	30.0	35.5	2.0	37.0
82-83	29.9447536009801	34.5	30.0	35.0	2.0	37.0
84-85	29.78257517859926	34.0	30.0	35.0	2.0	36.0
86-87	29.584175340472783	34.0	30.0	35.0	2.0	36.0
88-89	29.44047535677639	34.0	30.0	35.0	2.0	36.0
90-91	29.28996564062804	34.0	30.0	35.0	2.0	35.5
92-93	29.17146677399184	34.0	29.5	35.0	2.0	35.0
94-95	29.073438486537896	34.0	29.0	35.0	2.0	35.0
96-97	29.00996388570644	34.0	29.0	35.0	2.0	35.0
98-99	28.966875854797678	34.0	29.0	35.0	2.0	35.0
100-101	28.823256835544193	34.0	29.0	35.0	2.0	35.0
102-103	27.454293288366003	32.5	26.0	34.0	2.0	35.0
104-105	28.549105003824014	34.0	29.0	35.0	2.0	35.0
106-107	28.83773300113461	34.0	29.5	35.0	2.0	35.0
108-109	29.048413415240745	34.0	30.0	35.0	2.0	35.0
110-111	29.032114511679225	34.0	30.0	35.0	2.0	35.0
112-113	28.965850028385994	34.0	30.0	35.0	2.0	35.0
114-115	28.856855833744152	34.0	30.0	35.0	2.0	35.0
116-117	28.768807486484476	34.0	30.0	35.0	2.0	35.0
118-119	28.632887978150396	34.0	30.0	35.0	2.0	35.0
120-121	28.477243040310327	34.0	30.0	35.0	2.0	35.0
122-123	28.303088662016215	34.0	30.0	35.0	2.0	35.0
124-125	27.510477767717582	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	84686.0
3	28329.0
4	14194.0
5	11440.0
6	12408.0
7	14524.0
8	16974.0
9	18531.0
10	19797.0
11	21158.0
12	21878.0
13	22475.0
14	22869.0
15	19966.0
16	16198.0
17	15158.0
18	14547.0
19	14072.0
20	13969.0
21	14376.0
22	16054.0
23	19899.0
24	27517.0
25	36633.0
26	45798.0
27	54840.0
28	65683.0
29	78220.0
30	92796.0
31	111776.0
32	137383.0
33	174296.0
34	228869.0
35	328561.0
36	573005.0
37	902228.0
38	301273.0
39	317.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.64982604410244	19.155066605758787	14.189975519575338	25.00513183056344
2	16.055990427075233	20.163429511923113	38.33230593610466	25.448274124896997
3	19.10855935044146	24.07394643683105	29.031638381484715	27.785855831242774
4	13.611250307472206	16.72382576122076	35.590819455371395	34.074104475935634
5	14.524117990383994	36.9166069025045	34.01230407673303	14.546971030378478
6	33.39063663973411	35.661037931743735	16.851629005660293	14.096696422861859
7	28.845994131000495	31.194341398593657	21.02627207795803	18.933392392447814
8	26.41153835506464	35.14735763917726	19.551892146277993	18.88921185948011
9	26.173390893493863	15.920563434716994	19.34510321044721	38.56094246134194
10-11	25.619145664876392	25.61943636146432	27.37506379751903	21.38635417614026
12-13	26.103946010356726	22.87743580202616	27.50352164148361	23.515096546133503
14-15	23.496077337434812	24.404235672625532	24.715972096281398	27.383714893658258
16-17	23.6654884575003	27.36170209231342	25.462068236086317	23.510741214099966
18-19	23.692333803461896	26.614675652822516	26.862739072002768	22.830251471712824
20-21	24.570712720160458	25.704491312086	26.54432115323489	23.180474814518657
22-23	24.148521902468765	25.588436931865694	26.31913760154081	23.94390356412473
24-25	23.851154365885442	25.816737623527303	26.493911526187862	23.83819648439939
26-27	23.82800267684169	26.026997116885337	26.391255798139333	23.753744408133635
28-29	23.76254675094111	25.961654537540852	26.537811086881163	23.737987624636876
30-31	24.148464856965575	25.875595947927877	26.311002377561927	23.664936817544618
32-33	23.76780300020405	25.932486687463197	26.312668872571482	23.987041439761274
34-35	23.98349768414658	25.976002395524254	26.358422426005447	23.68207749432371
36-37	24.112798188964778	25.797242579263248	26.28900033115187	23.800958900620106
38-39	24.10522016627555	25.860043334094797	26.27571836991811	23.759018129711546
40-41	24.063110745747167	25.803493418949586	26.34819365661305	23.785202178690195
42-43	24.07388974130616	25.747928667556664	26.250887931455775	23.927293659681396
44-45	24.074508371725607	25.798055776062885	26.1482309467134	23.979204905498115
46-47	24.092405859782488	25.807395265352234	26.129526860566777	23.9706720142985
48-49	24.05510603234795	25.901078375780234	26.129417829914104	23.914397761957712
50-51	23.951638605051564	26.127427892489504	25.973545188905383	23.947388313553557
52-53	24.028137203963993	25.99346643835057	25.963217185894738	24.015179171790695
54-55	24.011182499634504	25.933937803748876	26.014194514466976	24.040685182149645
56-57	24.015059095069883	26.003325914723458	25.9219639399076	24.05965105029906
58-59	23.94237217180977	25.83128401040822	26.099892517834782	24.126451299947227
60-61	24.0200425670589	25.99058950697356	26.075563036154865	23.913804889812674
62-63	23.830619431854856	26.056610724536032	26.03337976295282	24.079390080656292
64-65	23.846602744220917	25.98122754078569	26.03343710173942	24.138732613253975
66-67	23.861514307391552	26.004507922091584	25.960010189897602	24.17396758061926
68-69	23.919620433053094	25.803929267155084	25.96690459976141	24.309545700030416
70-71	23.76579144376956	25.80286917153236	26.06923112389154	24.362108260806536
72-73	23.900184842883547	25.806683420216416	25.975174285378028	24.317957451522012
74-75	23.884934035894638	25.888928235915294	25.888831329634463	24.337306398555597
76-77	23.849473570845	25.7520881223773	26.007017763402146	24.391420543375553
78-79	23.89448914776608	25.84801030611293	25.912852656418327	24.344647889702664
80-81	23.80275216656976	25.90055929340754	25.89536783232273	24.401320707699973
82-83	23.758487542375413	25.83875912158159	25.98749530700234	24.415258029040658
84-85	23.779358525162415	25.844915198749057	25.831695392406072	24.544030883682456
86-87	23.6680513525639	25.74665826691257	26.02020398334944	24.565086397174092
88-89	23.67420842039838	25.741025926753924	25.920109412991827	24.66465623985587
90-91	23.635555959156452	25.813201200798797	25.930202393236414	24.62104044680834
92-93	23.58052052022397	25.799461513617693	26.01204305731465	24.607974908843687
94-95	23.48678731688734	25.731426274313506	26.02240209258956	24.759384316209594
96-97	23.616065854176476	25.61005288412421	26.006358498490666	24.767522763208643
98-99	23.53988595114953	25.63305639235861	25.916392784971933	24.91066487151993
100-101	23.403964619341753	25.73777188564645	25.992134216931547	24.86612927808025
102-103	23.31161391248159	25.61130426850226	26.00567849046449	25.071403328551657
104-105	23.275042954351278	25.775725406661287	25.833450074668313	25.11578156431912
106-107	23.1495793117329	25.75731778319113	25.982286547526794	25.110816357549176
108-109	23.19368134530126	25.74379211195267	25.94259624185169	25.11993030089438
110-111	23.09052015654721	25.855441045501067	25.86881239443763	25.18522640351409
112-113	23.02882641613586	25.895314783445457	25.7970388206663	25.27881997975238
114-115	22.975710211189917	25.953086931715475	25.7582409855548	25.312961871539812
116-117	22.875731502278228	25.98079335478478	25.775691043778092	25.367784099158897
118-119	22.9232759982112	25.9268958161504	25.704375214274872	25.445452971363537
120-121	22.81022031670431	25.99499328659718	25.744228556591402	25.450557840107102
122-123	22.725845823190024	26.089733191082487	25.62032201324439	25.564098972483094
124-125	22.67166722565911	26.166842488484804	25.55525121009632	25.606239075759767
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	33.0
1	53.0
2	105.5
3	164.5
4	156.0
5	130.0
6	149.0
7	287.5
8	428.5
9	454.5
10	406.5
11	335.5
12	334.5
13	365.5
14	419.0
15	463.0
16	520.5
17	628.0
18	739.5
19	815.5
20	968.0
21	1201.0
22	1438.0
23	1778.0
24	2355.5
25	3055.5
26	4149.5
27	5771.0
28	8038.0
29	11646.5
30	16322.5
31	22017.5
32	29287.0
33	37477.0
34	45895.0
35	54333.0
36	64121.5
37	73961.0
38	82800.0
39	91593.0
40	100129.0
41	107955.0
42	115073.0
43	123165.5
44	131894.0
45	139549.0
46	146159.5
47	151243.0
48	156287.5
49	161433.0
50	163755.0
51	161197.0
52	154267.0
53	149027.0
54	144991.5
55	137555.0
56	126838.5
57	115200.0
58	104854.5
59	93696.5
60	79792.0
61	66659.0
62	54523.0
63	41147.0
64	30210.0
65	22756.0
66	16902.0
67	12623.0
68	9359.5
69	6987.5
70	5458.0
71	4273.5
72	3330.0
73	2675.0
74	2002.0
75	1455.0
76	1089.0
77	728.0
78	462.0
79	294.0
80	196.5
81	114.5
82	69.0
83	45.5
84	26.0
85	13.5
86	8.5
87	6.0
88	4.0
89	6.5
90	7.0
91	4.0
92	2.5
93	3.0
94	2.5
95	3.5
96	3.5
97	1.0
98	1.0
99	1.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07955275518539197
2	2.2144121137200267E-4
3	7.750442398020093E-4
4	0.18490341149562226
5	0.0740167249010919
6	0.009411251483310115
7	0.013757035256485667
8	0.010989020114335633
9	0.019597547206422237
10-11	0.018919383496595478
12-13	0.020621712809017752
14-15	0.027458710210128336
16-17	0.027707831572921837
18-19	0.02543805915635881
20-21	0.02803999338997984
22-23	0.03030976580654287
24-25	0.027555590740103584
26-27	0.02707118809022733
28-29	0.02781855217860784
30-31	0.028067673541401342
32-33	0.024247812645234294
34-35	0.026614465591772572
36-37	0.029368640658211858
38-39	0.033825145037073415
40-41	0.02982536315666661
42-43	0.02968696239955911
44-45	0.02978384292953436
46-47	0.03126473103058463
48-49	0.026822066727433827
50-51	0.032662578677370394
52-53	0.0287181570998066
54-55	0.03213665580036189
56-57	0.029313280355368856
58-59	0.025880941579102815
60-61	0.02723726899875633
62-63	0.031486172241956635
64-65	0.03569355525802468
66-67	0.035347553365255927
68-69	0.030655767699311623
70-71	0.04001165887977874
72-73	0.042129190463523515
74-75	0.026572945364640325
76-77	0.019929709023480242
78-79	0.02380493022249029
80-81	0.027597110967235834
82-83	0.025825581276259814
84-85	0.019168504859388983
86-87	0.019002423950859982
88-89	0.019223865162231984
90-91	0.021258356291712258
92-93	0.025092057263590057
94-95	0.01973594796352974
96-97	0.023195966891217282
98-99	0.020372591446224247
100-101	0.020026589553455494
102-103	0.017867537742578467
104-105	0.020012749477744743
106-107	0.017715296909760217
108-109	0.016580410701478702
110-111	0.013729355105064168
112-113	0.011625663597030142
114-115	0.00985413390605412
116-117	0.009784933527500369
118-119	0.006158833691283825
120-121	0.004082822334671299
122-123	0.0082763652750286
124-125	0.056301427991331685
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3612697.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.878339972689815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.501301072485	35.36110824510163
2	14.846500655576236	15.107306155183394
3	5.948495296430902	9.07948698053273
4	3.2198610513617907	6.5528474094403055
5	1.834619647566423	4.667120107473041
6	1.1278037209337117	3.442846868167799
7	0.7944551935384706	2.8294393000943576
8	0.5349453490327559	2.177370506791821
9	0.3805079972981071	1.7423653723974384
>10	1.727503689774263	14.804721808777051
>50	0.06385839783674516	2.216463602010578
>100	0.019275255656922008	1.5159534424156114
>500	4.363362624244767E-4	0.13855615302202648
>1k	4.363362462697561E-4	0.36441404859216836
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.5360302843000675E-5
2	0.0	0.0	0.0	2.7680151421500338E-5	5.5360302843000675E-5
3	0.0	0.0	0.0	2.7680151421500338E-5	5.5360302843000675E-5
4	0.0	0.0	0.0	5.5360302843000675E-5	1.3840075710750167E-4
5	0.0	0.0	0.0	5.5360302843000675E-5	1.3840075710750167E-4
6	0.0	0.0	0.0	5.5360302843000675E-5	1.9376105995050236E-4
7	0.0	0.0	0.0	5.5360302843000675E-5	1.9376105995050236E-4
8	0.0	0.0	0.0	5.5360302843000675E-5	2.214412113720027E-4
9	0.0	0.0	0.0	5.5360302843000675E-5	2.49121362793503E-4
10-11	0.0	0.0	0.0	8.3040454264501E-5	2.49121362793503E-4
12-13	0.0	0.0	0.0	1.1072060568600135E-4	3.460018927687542E-4
14-15	0.0	0.0	0.0	1.1072060568600135E-4	4.844026498762559E-4
16-17	0.0	0.0	0.0	1.1072060568600135E-4	5.812831798515071E-4
18-19	0.0	0.0	0.0	1.3840075710750167E-4	6.504835584052579E-4
20-21	0.0	0.0	0.0	1.3840075710750167E-4	6.781637098267582E-4
22-23	0.0	0.0	0.0	3.32161817058004E-4	8.027243912235097E-4
24-25	0.0	0.0	0.0	5.536030284300068E-4	8.719247697772606E-4
26-27	0.0	0.0	0.0	8.99604921198761E-4	9.549652240417616E-4
28-29	0.0	0.0	0.0	0.0018407300695297724	9.82645375463262E-4
30-31	0.0	0.0	0.0	0.007058438612482586	9.96485451174012E-4
32-33	0.0	0.0	0.0	0.01894706364801698	0.0010518457540170127
34-35	0.0	0.0	0.0	0.03472474995827217	0.0010518457540170127
36-37	0.0	0.0	0.0	0.06225266054695426	0.0010656858297277629
38-39	0.0	0.0	0.0	0.10746818789397505	0.0011764064354137644
40-41	0.0	0.0	0.0	0.16460002042795174	0.0012732869653890155
42-43	0.0	0.0	0.0	0.21665254517608312	0.0012732869653890155
44-45	0.0	0.0	0.0	0.27891904579874816	0.0012871270410997656
46-47	0.0	0.0	0.0	0.35299113100268303	0.001411687722496517
48-49	0.0	0.0	0.0	0.4338310132291748	0.001467048025339518
50-51	0.0	0.0	0.0	0.5193626811216108	0.001467048025339518
52-53	0.0	0.0	0.0	0.6070810809763454	0.0015362484038932687
54-55	0.0	0.0	0.0	0.7069510673051186	0.0016054487824470195
56-57	0.0	0.0	0.0	0.8174225516283264	0.00164696900957927
58-59	0.0	2.7680151421500338E-5	0.0	0.9409867475739039	0.0017023293124222706
60-61	0.0	4.15202271322505E-5	0.0	1.0756368441637925	0.0017853697666867718
62-63	0.0	5.5360302843000675E-5	0.0	1.2148403256625175	0.0019099304480835232
64-65	0.0	5.5360302843000675E-5	0.0	1.365378829168347	0.0019237705237942734
66-67	0.0	5.5360302843000675E-5	0.0	1.5389887388839971	0.0019376105995050235
68-69	0.0	6.920037855375085E-5	0.0	1.732223875957491	0.0019514506752157737
70-71	0.0	8.3040454264501E-5	0.0	1.9395482101045285	0.0021313716594555257
72-73	0.0	8.3040454264501E-5	0.0	2.1547337072552724	0.0023666529465382787
74-75	0.0	8.3040454264501E-5	0.0	2.386029052533329	0.0024220132493812793
76-77	0.0	8.3040454264501E-5	0.0	2.643149979087646	0.0024358533250920297
78-79	0.0	8.3040454264501E-5	0.0	2.9321584400795304	0.0024358533250920297
80-81	0.0	8.3040454264501E-5	0.0	3.2408059685049704	0.0024912136279350303
82-83	0.0	8.3040454264501E-5	0.0	3.568040718609947	0.002781855217860784
84-85	0.0	8.3040454264501E-5	0.0	3.9050742423181353	0.002795695293571534
86-87	1.3840075710750169E-5	8.3040454264501E-5	0.0	4.266618540110062	0.0028095353692822837
88-89	2.7680151421500338E-5	8.3040454264501E-5	0.0	4.664991279368294	0.002823375444993034
90-91	2.7680151421500338E-5	8.3040454264501E-5	0.0	5.096718601089435	0.002823375444993034
92-93	2.7680151421500338E-5	8.3040454264501E-5	0.0	5.543074882836839	0.0028510555964145344
94-95	2.7680151421500338E-5	8.3040454264501E-5	0.0	5.998164252357726	0.0030032964292327864
96-97	2.7680151421500338E-5	8.3040454264501E-5	0.0	6.472837329009325	0.003058656732075787
98-99	2.7680151421500338E-5	9.688052997525118E-5	0.0	6.9907744823327285	0.0030863368834972878
100-101	2.7680151421500338E-5	1.1072060568600135E-4	0.0	7.554854448075773	0.0031001769592080377
102-103	2.7680151421500338E-5	1.1072060568600135E-4	0.0	8.13006183469026	0.003210897564894039
104-105	2.7680151421500338E-5	1.1072060568600135E-4	0.0	8.71249374082576	0.0033216181705800403
106-107	2.7680151421500338E-5	1.1072060568600135E-4	0.0	9.311865899631217	0.0034184987005552916
108-109	2.7680151421500338E-5	1.1072060568600135E-4	0.0	9.950986755877949	0.0035015391548197925
110-111	2.7680151421500338E-5	1.1072060568600135E-4	0.0	10.617427921577702	0.003667620063348795
112-113	2.7680151421500338E-5	1.1072060568600135E-4	0.0	11.315728941563602	0.0037368204419025454
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	9570	0.0	33.780727	1
TCAACGC	11170	0.0	28.241104	4
GGTATCA	6985	0.0	27.837667	1
CAACGCA	11335	0.0	27.727764	5
ATCAACG	11540	0.0	27.489887	3
TATCAAC	11805	0.0	26.971197	2
AACGCAG	11980	0.0	26.268217	6
GTACATG	15655	0.0	24.689005	1
TACATGG	15530	0.0	23.995703	2
CATGGGG	9680	0.0	23.347643	4
ACATGGG	15540	0.0	23.291895	3
ACGCAGA	13850	0.0	22.678898	7
CGCAGAG	14365	0.0	21.700787	8
GCAGAGT	15750	0.0	19.301994	9
AGAGTAC	14080	0.0	18.507011	10-11
GTATAGG	1120	0.0	18.106836	1
GAGTACT	5455	0.0	17.06807	12-13
TACCGTA	295	5.2425563E-5	16.132652	7
ATGGGGA	5030	0.0	16.094347	5
CAGAGTA	14965	0.0	15.981376	10-11
>>END_MODULE