FastQCFastQC Report
Mon 15 Aug 2016
SRR2921112_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921112_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3128818
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA78300.034.366561
TCAACGC87950.029.6358384
CAACGCA88150.029.5006165
ATCAACG90150.029.0441673
TATCAAC91750.028.4728162
AACGCAG91350.028.2699726
GTACATG142650.027.8781621
TACATGG143250.026.6693482
GGTATCA57050.026.6099471
ACATGGG146150.025.8958573
CATGGGG91800.025.2813824
ACGCAGA105200.024.0956177
CGCAGAG108550.023.2971768
GCAGAGT123150.019.9553729
ATGGGGA50750.019.6990495
TAATACG3101.9520667E-719.1962874
GTATAGC11300.018.9663371
GTATATA14200.018.8661651
TATACTG13550.017.5668015
TGTACCG10200.016.9188085
Produced by FastQC (version 0.11.5)