FastQCFastQC Report
Mon 15 Aug 2016
SRR2921112_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921112_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3128818
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA93900.036.2781371
GGTATCA67450.032.9914441
TCAACGC111600.030.1443464
ATCAACG112500.030.0082663
CAACGCA113550.029.5674345
TATCAAC118250.028.6467572
AACGCAG118250.028.4742436
ACGCAGA135650.024.558697
GTACATG145450.024.486971
CGCAGAG139750.023.8815128
TACATGG146750.023.4897292
CATGGGG91150.022.6417714
ACATGGG147150.022.617833
GTATAGC12350.021.2549571
GCAGAGT153000.021.0363589
GAGTACT56150.019.76015312-13
AGAGTAC139900.018.73222510-11
GTATAGG8200.018.1886581
GTACTTT60800.018.1025514-15
CAGAGTA148600.017.89575210-11
Produced by FastQC (version 0.11.5)