##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921112_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3128818 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.733518216783462 31.0 31.0 34.0 27.0 34.0 2 30.822010100939078 31.0 31.0 34.0 27.0 34.0 3 30.90542147226205 31.0 31.0 34.0 27.0 34.0 4 34.42951267858981 37.0 35.0 37.0 31.0 37.0 5 34.097944655138136 37.0 35.0 37.0 30.0 37.0 6 33.993570095799754 37.0 35.0 37.0 30.0 37.0 7 33.81993199987983 37.0 35.0 37.0 29.0 37.0 8 33.81533569546071 37.0 35.0 37.0 29.0 37.0 9 35.24056113203133 39.0 35.0 39.0 28.0 39.0 10-11 35.208219525712266 39.0 35.0 39.0 28.5 39.0 12-13 35.087843716061464 39.0 35.0 39.0 27.0 39.0 14-15 36.203001420983895 40.0 36.0 41.0 27.5 41.0 16-17 36.16204697748479 40.0 36.0 41.0 27.0 41.0 18-19 36.20560192379358 40.0 36.0 41.0 27.0 41.0 20-21 36.206283810691446 40.0 36.0 41.0 27.0 41.0 22-23 36.17718991644768 40.0 36.0 41.0 27.0 41.0 24-25 36.08281593879862 40.0 36.0 41.0 27.0 41.0 26-27 35.988420227702605 40.0 36.0 41.0 27.0 41.0 28-29 35.89760558140486 40.0 35.0 41.0 27.0 41.0 30-31 35.82004210535736 39.0 35.0 41.0 27.0 41.0 32-33 35.701769486112646 39.0 35.0 41.0 27.0 41.0 34-35 35.59267573249707 39.0 35.0 41.0 26.5 41.0 36-37 35.468289782275605 39.0 35.0 41.0 26.0 41.0 38-39 35.31004264230134 39.0 35.0 41.0 25.0 41.0 40-41 35.171155529020865 39.0 35.0 41.0 25.0 41.0 42-43 35.04670453826333 39.0 34.5 41.0 24.5 41.0 44-45 34.89477815584032 39.0 34.0 41.0 24.0 41.0 46-47 34.70460681957212 39.0 34.0 41.0 24.0 41.0 48-49 34.52038565362383 39.0 34.0 41.0 23.0 41.0 50-51 34.310041204058535 38.0 33.0 40.5 22.5 41.0 52-53 34.13994997471889 38.0 33.0 40.0 22.0 41.0 54-55 33.932558397452325 38.0 33.0 40.0 21.5 41.0 56-57 33.66359964050322 38.0 33.0 40.0 20.0 41.0 58-59 33.43462643081189 37.0 33.0 40.0 19.5 41.0 60-61 33.163842863343284 37.0 32.0 40.0 18.5 41.0 62-63 32.86367615502084 36.5 32.0 40.0 18.0 41.0 64-65 32.57104759688802 36.0 32.0 39.0 16.5 41.0 66-67 32.23565097106959 35.5 31.5 39.0 14.0 41.0 68-69 31.876394056797167 35.0 31.0 39.0 13.0 40.0 70-71 31.517241335226274 35.0 31.0 38.0 9.5 40.0 72-73 31.31933944384109 35.0 31.0 37.0 9.0 39.5 74-75 31.11513245577084 35.0 31.0 37.0 8.0 39.0 76-77 30.88004927100266 35.0 31.0 36.0 7.5 39.0 78-79 30.556035857630583 35.0 31.0 36.0 7.0 38.5 80-81 30.26007089578237 35.0 30.0 35.5 6.5 37.0 82-83 30.043429659379356 35.0 30.0 35.0 2.0 37.0 84-85 29.889230373898386 34.0 30.0 35.0 2.0 36.0 86-87 29.689244948092217 34.0 30.0 35.0 2.0 36.0 88-89 29.548421640376652 34.0 30.0 35.0 2.0 36.0 90-91 29.39135162224201 34.0 30.0 35.0 2.0 35.5 92-93 29.27767946234009 34.0 30.0 35.0 2.0 35.0 94-95 29.17693311020328 34.0 30.0 35.0 2.0 35.0 96-97 29.112967261119053 34.0 29.5 35.0 2.0 35.0 98-99 29.073296689037203 34.0 29.5 35.0 2.0 35.0 100-101 28.931060227856015 34.0 29.0 35.0 2.0 35.0 102-103 27.542749210724306 32.5 26.0 34.0 2.0 35.0 104-105 28.64534178721805 34.0 29.0 35.0 2.0 35.0 106-107 28.929504368742442 34.0 29.5 35.0 2.0 35.0 108-109 29.138342019254555 34.0 30.0 35.0 2.0 35.0 110-111 29.119590049660925 34.0 30.0 35.0 2.0 35.0 112-113 29.04616519720866 34.0 30.0 35.0 2.0 35.0 114-115 28.931800763099677 34.0 30.0 35.0 2.0 35.0 116-117 28.83726106791766 34.0 30.0 35.0 2.0 35.0 118-119 28.69946190542243 34.0 30.0 35.0 2.0 35.0 120-121 28.528991779004084 34.0 30.0 35.0 2.0 35.0 122-123 28.34951505648459 34.0 30.0 35.0 2.0 35.0 124-125 27.55153863216077 33.5 27.5 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 69095.0 3 22690.0 4 11421.0 5 9106.0 6 10157.0 7 12162.0 8 14055.0 9 15556.0 10 16711.0 11 17665.0 12 18703.0 13 19676.0 14 20754.0 15 18198.0 16 14170.0 17 12892.0 18 12258.0 19 11856.0 20 11920.0 21 12499.0 22 13862.0 23 16950.0 24 23216.0 25 31193.0 26 39231.0 27 47802.0 28 56264.0 29 68054.0 30 80806.0 31 97668.0 32 120877.0 33 152903.0 34 201032.0 35 289800.0 36 500473.0 37 777434.0 38 259413.0 39 296.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.495957571696124 19.005538646574212 13.57024388917589 24.928259892553776 2 16.37398359952071 20.29397109636856 37.65059634474566 25.681448959365078 3 19.61583969307063 23.851076355840167 28.963007519181488 27.570076431907715 4 13.742107150633348 16.48359952354727 35.38039371389782 34.39389961192156 5 14.466697074163374 36.950290139974705 33.89740732377139 14.685605462090532 6 33.60949987326385 35.31348578109902 16.809496549036982 14.267517796600146 7 29.049949143689695 31.345739161788277 20.612244767489805 18.992066927032223 8 26.864598699382935 34.54210703800366 19.447693234649666 19.145601027963746 9 26.41973856326153 15.740995290660727 19.15366792700844 38.685598219069306 10-11 25.747093110606606 25.59453216454653 27.2128929263507 21.44548179849616 12-13 26.29927847077105 22.855479655023935 27.23933473924603 23.605907134958986 14-15 23.57156900826248 24.370355451335442 24.774288322072127 27.28378721832995 16-17 23.69699291856227 27.34140535072866 25.46130209514712 23.50029963556195 18-19 23.6849289887389 26.464574292085263 27.069093117861907 22.78140360131393 20-21 24.65094190178978 25.63366111420774 26.701963440313293 23.01343354368919 22-23 24.101661227178568 25.410703064693436 26.560132945490402 23.927502762637598 24-25 23.77323661630205 25.75003840366706 26.78127077534486 23.69545420468603 26-27 23.688956713652562 25.862585160368678 26.70390670319059 23.74455142278817 28-29 23.643396106980976 25.90789262515673 26.791791961401017 23.656919306461276 30-31 24.025613015079248 25.76199487138664 26.586115275192213 23.6262768383419 32-33 23.664430354090996 25.825446138284807 26.558646780470745 23.951476727153455 34-35 23.835842061986977 25.92975986025629 26.642081296363312 23.59231678139342 36-37 24.002220015208255 25.730484590891706 26.5438880605926 23.723407333307435 38-39 23.89473857531383 25.817810887072216 26.50245519510716 23.78499534250679 40-41 23.95413904487355 25.803277257716424 26.571494932925223 23.671088764484807 42-43 23.996656560899794 25.616648395721498 26.51413064988617 23.87256439349254 44-45 23.973581454650493 25.739794560434493 26.408730390262917 23.877893594652093 46-47 24.009721668950444 25.742488949306523 26.305446091222812 23.94234329052022 48-49 23.91633980576006 25.892946486030795 26.254680725974882 23.936032982234263 50-51 23.841228775789723 26.06915540527579 26.202987662878268 23.886628156056215 52-53 24.05294486806463 25.908547273870635 26.09280633568558 23.945701522379157 54-55 23.95039731416297 25.848863479595735 26.11075560906825 24.089983597173042 56-57 23.82221143758613 25.96097421710016 26.046782408617393 24.17003193669632 58-59 23.74740609761673 25.855136606064754 26.233939863983768 24.163517432334753 60-61 23.954194785811758 25.951359413705532 26.225433661835602 23.869012138647104 62-63 23.665651487792278 25.967819490411326 26.250233386703226 24.116295635093177 64-65 23.656097046686686 25.90798226359034 26.180179912302705 24.25574077742027 66-67 23.719717743140887 26.030763318374078 26.035910840441684 24.21360809804335 68-69 23.850801949353073 25.78190001980556 26.100019837530464 24.2672781933109 70-71 23.67293392710521 25.69401990551149 26.214838584258228 24.418207583125078 72-73 23.767383867872933 25.769107606399928 26.088051991611845 24.375456534115298 74-75 23.699811141153408 25.81509416168012 26.098358454570374 24.3867362425961 76-77 23.681691455087467 25.72029685048293 26.142962427932755 24.455049266496847 78-79 23.67599592915372 25.837693945822966 26.039797685269622 24.446512439753697 80-81 23.726987240700346 25.76361264235566 26.065259736834452 24.44414038010955 82-83 23.672090940071698 25.78184791872733 26.120526668793666 24.425534472407303 84-85 23.618674524038425 25.82291024838789 25.93666312637406 24.621752101199622 86-87 23.54462690240932 25.769370930258578 26.00717009721028 24.678832070121825 88-89 23.543241051807684 25.714344167378055 25.955004225176747 24.787410555637514 90-91 23.43360302791035 25.813990659254593 26.045127755951448 24.707278556883605 92-93 23.50211447293064 25.752037708700005 26.09095118753985 24.654896630829505 94-95 23.30941150709104 25.716655824591744 26.12658215708808 24.84735051122914 96-97 23.396396024879827 25.623873737795126 26.105722954442506 24.87400728288254 98-99 23.268559347011216 25.667239508711226 25.97098644550872 25.09321469876884 100-101 23.233518596925975 25.65946310799456 26.058968197988296 25.048050097091163 102-103 23.20687217490635 25.640586313668447 26.01955902606682 25.13298248535838 104-105 23.10350724929553 25.76380930460787 25.8713619660927 25.2613214800039 106-107 23.030416074094887 25.77918194247187 25.948474465310856 25.241927518122388 108-109 22.96105863859869 25.777696442941707 25.97323036129982 25.288014557159787 110-111 22.88200186450546 25.908235197471203 25.809943507050708 25.39981943097262 112-113 22.8155373942306 26.01821829219333 25.768274549113567 25.397969764462502 114-115 22.811241231572158 26.110385181141194 25.697974207892848 25.380399379393804 116-117 22.6638894478096 26.029474681477787 25.791840595777693 25.51479527493492 118-119 22.64689933995799 26.019967944789222 25.69096175061276 25.642170964640027 120-121 22.550777203562994 26.163008670966402 25.659911075370513 25.62630305010009 122-123 22.480769688704765 26.23172519117641 25.551290765375263 25.73621435474357 124-125 22.448199052761662 26.314796453764288 25.490947575709754 25.746056917764292 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 37.0 1 46.5 2 100.5 3 160.0 4 151.5 5 119.0 6 159.5 7 333.0 8 518.5 9 582.5 10 509.5 11 410.0 12 385.5 13 418.0 14 471.0 15 539.0 16 633.0 17 753.5 18 872.5 19 939.0 20 1062.0 21 1273.0 22 1554.5 23 2000.5 24 2524.0 25 3168.5 26 4265.5 27 5901.5 28 7804.0 29 10577.5 30 14485.0 31 19719.5 32 26657.0 33 34041.5 34 41425.5 35 48400.0 36 56936.5 37 64940.5 38 71618.5 39 78066.5 40 83959.5 41 90713.0 42 97126.5 43 104262.5 44 111207.5 45 118202.5 46 125402.5 47 129526.0 48 131849.0 49 135306.5 50 139432.5 51 138743.5 52 133942.0 53 130927.0 54 127578.0 55 120244.0 56 110566.5 57 101643.0 58 94630.5 59 85286.5 60 71595.5 61 58542.5 62 47161.5 63 35552.0 64 25884.5 65 19496.5 66 14424.0 67 10393.5 68 7513.5 69 5577.0 70 4316.5 71 3222.0 72 2409.0 73 2200.0 74 1806.5 75 1352.5 76 935.0 77 528.0 78 317.0 79 165.0 80 116.5 81 80.5 82 43.0 83 25.5 84 14.5 85 9.0 86 6.0 87 4.5 88 4.5 89 2.0 90 1.0 91 2.0 92 1.5 93 2.0 94 3.0 95 3.5 96 5.5 97 5.0 98 3.5 99 2.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08319435646304771 2 2.2372665971622508E-4 3 6.392190277606431E-4 4 0.18339193906452853 5 0.07657843952572504 6 0.008661417826156715 7 0.012656536749660734 8 0.009684168270573744 9 0.0180898984856262 10-11 0.017898132777298008 12-13 0.019208531784207325 14-15 0.026303862992350463 16-17 0.02721475010690938 18-19 0.0253450344507095 20-21 0.027630242474953805 22-23 0.029388094801295568 24-25 0.027534359620789702 26-27 0.02628788251665645 28-29 0.027486418193707656 30-31 0.0280297543673042 32-33 0.024034635443800183 34-35 0.025760526818753918 36-37 0.02913240719019131 38-39 0.03395851084978417 40-41 0.02967574336378786 42-43 0.02879681720061697 44-45 0.03002731382905621 46-47 0.03068251333251087 48-49 0.026559550603454725 50-51 0.032664092318568864 52-53 0.02828544197840846 54-55 0.032072814717890266 56-57 0.02913240719019131 58-59 0.025856409672918013 60-61 0.026639452981924803 62-63 0.030746435235286935 64-65 0.03603597269000626 66-67 0.03514106605114136 68-69 0.029036524336027214 70-71 0.03969550162393594 72-73 0.04198070964818024 74-75 0.026223960613880385 76-77 0.01842548847520054 78-79 0.023059826426465202 80-81 0.026367784895126534 82-83 0.024929542082665082 84-85 0.017434698982171544 86-87 0.017514601360641622 88-89 0.017466659933559576 90-91 0.018793039416162907 92-93 0.023443357843121588 94-95 0.01831362514534243 96-97 0.021126188867489258 98-99 0.01777028897174588 100-101 0.01829764466964841 102-103 0.015740768558605837 104-105 0.018633234659222748 106-107 0.01614028045095624 108-109 0.014718018114188809 110-111 0.011969376294818044 112-113 0.010035738735842098 114-115 0.008437691166440489 116-117 0.008581515447686636 118-119 0.0049060060380629366 120-121 0.0032280560901912477 122-123 0.007303077392165349 124-125 0.055212543522825556 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3128818.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.74641817338927 #Duplication Level Percentage of deduplicated Percentage of total 1 70.14417526112244 35.595656502284264 2 14.78434341189487 15.005049463980196 3 5.899064616156986 8.980691995300395 4 3.0218772102746816 6.133977783249359 5 1.7573395787775812 4.458934456864744 6 1.0825930921176043 3.296263305853349 7 0.6956717072196209 2.471199315617376 8 0.49267376035760985 2.000114293293075 9 0.37815714763008157 1.7271108674003 >10 1.6352500451687981 14.128268039334369 >50 0.07462399232888954 2.5731059944261805 >100 0.03246202812147459 2.8184939150814605 >500 0.0013892597950040018 0.44882837207632376 >1k 3.788890343474995E-4 0.36230569523861883 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 3.1960951388032156E-5 6 0.0 0.0 0.0 0.0 3.1960951388032156E-5 7 0.0 0.0 0.0 0.0 3.1960951388032156E-5 8 0.0 0.0 0.0 0.0 3.1960951388032156E-5 9 0.0 0.0 0.0 0.0 3.1960951388032156E-5 10-11 0.0 0.0 0.0 3.1960951388032156E-5 3.1960951388032156E-5 12-13 0.0 0.0 0.0 6.392190277606431E-5 3.1960951388032156E-5 14-15 0.0 0.0 0.0 9.588285416409647E-5 6.392190277606431E-5 16-17 0.0 0.0 0.0 1.5980475694016077E-4 6.392190277606431E-5 18-19 0.0 0.0 0.0 2.07746184022209E-4 6.392190277606431E-5 20-21 0.0 0.0 0.0 3.036290381863055E-4 6.392190277606431E-5 22-23 0.0 0.0 0.0 5.273556979025305E-4 1.1186332985811255E-4 24-25 0.0 0.0 0.0 0.001054711395805061 1.2784380555212863E-4 26-27 0.0 0.0 0.0 0.001773832802035785 1.2784380555212863E-4 28-29 0.0 0.0 0.0 0.004602376999876631 1.2784380555212863E-4 30-31 0.0 0.0 0.0 0.01353546291283162 1.4382428124614472E-4 32-33 0.0 0.0 0.0 0.030746435235286935 1.598047569401608E-4 34-35 0.0 0.0 0.0 0.054669207349229004 1.598047569401608E-4 36-37 0.0 0.0 0.0 0.0953075570391119 1.598047569401608E-4 38-39 0.0 0.0 0.0 0.15988465931863088 1.598047569401608E-4 40-41 0.0 0.0 0.0 0.23758173214293704 1.598047569401608E-4 42-43 0.0 0.0 0.0 0.31110790081110506 1.7578523263417687E-4 44-45 0.0 0.0 0.0 0.3938548039547203 1.9176570832819294E-4 46-47 0.0 0.0 0.0 0.49328532372288836 2.07746184022209E-4 48-49 0.0 1.5980475694016078E-5 0.0 0.5967109624145603 2.237266597162251E-4 50-51 0.0 3.1960951388032156E-5 0.0 0.7069602642275773 2.237266597162251E-4 52-53 0.0 3.1960951388032156E-5 0.0 0.8213325287696503 2.237266597162251E-4 54-55 0.0 3.1960951388032156E-5 0.0 0.9461400439399159 2.237266597162251E-4 56-57 0.0 3.1960951388032156E-5 0.0 1.092217572258917 2.3970713541024118E-4 58-59 0.0 3.1960951388032156E-5 0.0 1.2524058606157342 2.5568761110425725E-4 60-61 0.0 3.1960951388032156E-5 0.0 1.41732436977798 2.5568761110425725E-4 62-63 0.0 3.1960951388032156E-5 0.0 1.5891784053914288 2.5568761110425725E-4 64-65 1.5980475694016078E-5 3.1960951388032156E-5 0.0 1.7778758623863709 2.5568761110425725E-4 66-67 3.1960951388032156E-5 3.1960951388032156E-5 0.0 1.9932926747417077 2.5568761110425725E-4 68-69 3.1960951388032156E-5 3.1960951388032156E-5 0.0 2.2318012744748974 2.5568761110425725E-4 70-71 3.1960951388032156E-5 3.1960951388032156E-5 0.0 2.486577998464596 2.5568761110425725E-4 72-73 3.1960951388032156E-5 3.1960951388032156E-5 0.0 2.7543468491935297 2.5568761110425725E-4 74-75 3.1960951388032156E-5 3.1960951388032156E-5 0.0 3.034133017644363 2.5568761110425725E-4 76-77 3.1960951388032156E-5 3.1960951388032156E-5 0.0 3.34918809595189 2.5568761110425725E-4 78-79 3.1960951388032156E-5 3.1960951388032156E-5 0.0 3.6999275764841544 2.5568761110425725E-4 80-81 3.1960951388032156E-5 3.1960951388032156E-5 0.0 4.070754514963798 2.5568761110425725E-4 82-83 3.1960951388032156E-5 3.1960951388032156E-5 0.0 4.461173516644305 2.876485624922894E-4 84-85 3.1960951388032156E-5 3.1960951388032156E-5 0.0 4.864472781734189 2.876485624922894E-4 86-87 3.1960951388032156E-5 3.1960951388032156E-5 0.0 5.29768749732327 3.036290381863055E-4 88-89 3.1960951388032156E-5 3.1960951388032156E-5 0.0 5.7719400744945855 3.196095138803216E-4 90-91 3.1960951388032156E-5 3.1960951388032156E-5 0.0 6.273023870356154 3.196095138803216E-4 92-93 3.1960951388032156E-5 3.1960951388032156E-5 0.0 6.79280482277972 3.196095138803216E-4 94-95 3.1960951388032156E-5 3.1960951388032156E-5 0.0 7.326105257640425 3.5157046526835373E-4 96-97 3.1960951388032156E-5 3.1960951388032156E-5 0.0 7.882561401781759 3.5157046526835373E-4 98-99 3.1960951388032156E-5 3.1960951388032156E-5 0.0 8.48023119273796 3.5157046526835373E-4 100-101 3.1960951388032156E-5 3.1960951388032156E-5 0.0 9.132921761508658 3.5157046526835373E-4 102-103 3.1960951388032156E-5 3.1960951388032156E-5 0.0 9.796255327091572 4.314728437384342E-4 104-105 3.1960951388032156E-5 3.1960951388032156E-5 0.0 10.468969431906874 4.474533194324502E-4 106-107 3.1960951388032156E-5 3.1960951388032156E-5 0.0 11.154947331548208 4.6343379512646626E-4 108-109 3.1960951388032156E-5 3.1960951388032156E-5 0.0 11.87748855957745 4.7941427082048236E-4 110-111 3.1960951388032156E-5 3.1960951388032156E-5 0.0 12.64004489874451 5.593166492905627E-4 112-113 3.1960951388032156E-5 3.1960951388032156E-5 0.0 13.426859600015085 6.07258076372611E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9390 0.0 36.278137 1 GGTATCA 6745 0.0 32.991444 1 TCAACGC 11160 0.0 30.144346 4 ATCAACG 11250 0.0 30.008266 3 CAACGCA 11355 0.0 29.567434 5 TATCAAC 11825 0.0 28.646757 2 AACGCAG 11825 0.0 28.474243 6 ACGCAGA 13565 0.0 24.55869 7 GTACATG 14545 0.0 24.48697 1 CGCAGAG 13975 0.0 23.881512 8 TACATGG 14675 0.0 23.489729 2 CATGGGG 9115 0.0 22.641771 4 ACATGGG 14715 0.0 22.61783 3 GTATAGC 1235 0.0 21.254957 1 GCAGAGT 15300 0.0 21.036358 9 GAGTACT 5615 0.0 19.760153 12-13 AGAGTAC 13990 0.0 18.732225 10-11 GTATAGG 820 0.0 18.188658 1 GTACTTT 6080 0.0 18.10255 14-15 CAGAGTA 14860 0.0 17.895752 10-11 >>END_MODULE