FastQCFastQC Report
Mon 15 Aug 2016
SRR2921111_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921111_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2435889
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA78900.029.1292551
GTACATG119100.025.2463551
TACATGG118200.025.0237752
CATGGGG65450.024.8227064
TCAACGC92250.024.320424
AACGCAG92150.024.2146686
ATCAACG94600.023.8430613
CAACGCA93600.023.8424855
TATCAAC95600.023.6559092
GGTATCA61850.023.5855221
ACATGGG122250.023.5619073
ACGCAGA102800.021.4745167
CGCAGAG104250.020.9475178
GTATAGG5150.020.8105871
ATGGGGC29150.019.3946135
GTATAAT16750.018.8399681
GTATACG1908.286499E-418.802551
GCAGAGT114250.018.3845149
GTATTCG2000.001169376317.8624231
GAGTACT36450.017.71338512-13
Produced by FastQC (version 0.11.5)