##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921111_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2435889
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.330052806182877	31.0	30.0	34.0	23.0	34.0
2	29.405373561767387	31.0	30.0	34.0	23.0	34.0
3	29.505140833592993	31.0	30.0	34.0	23.0	34.0
4	32.925197330420225	37.0	35.0	37.0	26.0	37.0
5	33.01525890547558	37.0	35.0	37.0	26.0	37.0
6	33.00013383204243	37.0	35.0	37.0	26.0	37.0
7	32.923056428269106	37.0	35.0	37.0	25.0	37.0
8	32.83117826797527	37.0	35.0	37.0	25.0	37.0
9	34.20539729027062	39.0	35.0	39.0	25.0	39.0
10-11	34.14789528586894	38.5	35.0	39.0	25.0	39.0
12-13	34.11113971121016	39.0	35.0	39.0	25.0	39.0
14-15	35.12725046174107	39.0	35.0	41.0	24.5	41.0
16-17	35.02855528310198	39.0	34.5	41.0	24.0	41.0
18-19	35.11324161322622	39.5	35.0	41.0	24.0	41.0
20-21	35.15343268925636	40.0	35.0	41.0	24.0	41.0
22-23	35.13615337152062	39.0	35.0	41.0	24.0	41.0
24-25	35.06065998902248	39.0	34.5	41.0	24.0	41.0
26-27	35.00223676037783	39.0	34.0	41.0	24.0	41.0
28-29	34.86700625521114	39.0	34.0	41.0	23.5	41.0
30-31	34.763970361539464	39.0	34.0	41.0	23.0	41.0
32-33	34.65481349930148	39.0	34.0	41.0	23.0	41.0
34-35	34.54571883201575	39.0	34.0	41.0	22.0	41.0
36-37	34.4100872412495	39.0	34.0	41.0	20.5	41.0
38-39	34.248897219865114	39.0	33.0	41.0	19.0	41.0
40-41	34.13695184797008	39.0	33.0	41.0	18.0	41.0
42-43	34.05212306472093	39.0	33.0	41.0	17.5	41.0
44-45	33.896728873934734	38.5	33.0	41.0	16.0	41.0
46-47	33.664876765731115	38.0	33.0	40.0	15.0	41.0
48-49	33.528487135497556	38.0	33.0	40.0	13.0	41.0
50-51	33.37761347089297	38.0	33.0	40.0	9.0	41.0
52-53	33.18328236631472	38.0	32.5	40.0	8.5	41.0
54-55	32.947624460720505	38.0	32.0	40.0	8.0	41.0
56-57	32.69427322016726	38.0	32.0	40.0	6.5	41.0
58-59	32.47880547923161	37.0	31.0	40.0	2.0	41.0
60-61	32.19953639102603	37.0	31.0	40.0	2.0	41.0
62-63	31.934107219171317	36.0	31.0	40.0	2.0	41.0
64-65	31.619840230815115	36.0	31.0	39.0	2.0	41.0
66-67	31.339421459680636	35.5	30.0	39.0	2.0	41.0
68-69	30.979424555059776	35.0	30.0	39.0	2.0	40.0
70-71	30.655593296738893	35.0	30.0	38.0	2.0	40.0
72-73	30.292492186630838	35.0	29.0	37.0	2.0	39.5
74-75	30.225015179263096	35.0	30.0	37.0	2.0	39.0
76-77	29.96665940032571	35.0	29.5	36.5	2.0	39.0
78-79	29.693799881685905	35.0	29.0	36.0	2.0	39.0
80-81	29.347372971428502	34.0	29.0	36.0	2.0	37.5
82-83	29.08076106916202	34.0	29.0	35.0	2.0	37.0
84-85	28.88292611034411	34.0	29.0	35.0	2.0	37.0
86-87	28.661761475995007	34.0	29.0	35.0	2.0	36.0
88-89	28.494064589971053	34.0	29.0	35.0	2.0	36.0
90-91	28.333785324372336	34.0	29.0	35.0	2.0	36.0
92-93	28.486949939016103	34.0	29.0	35.0	2.0	35.0
94-95	28.4568956138806	34.0	29.0	35.0	2.0	35.0
96-97	28.392310774423628	34.0	29.0	35.0	2.0	35.0
98-99	28.283253465161998	34.0	29.0	35.0	2.0	35.0
100-101	28.187517986246498	34.0	29.0	35.0	2.0	35.0
102-103	26.914414203602874	32.5	26.0	34.5	2.0	35.0
104-105	27.894400771135302	34.0	28.0	35.0	2.0	35.0
106-107	28.05572175086796	34.0	29.0	35.0	2.0	35.0
108-109	28.001754595550125	34.0	29.0	35.0	2.0	35.0
110-111	27.977060736347184	34.0	29.0	35.0	2.0	35.0
112-113	27.899954390368364	34.0	29.0	35.0	2.0	35.0
114-115	27.772666570603178	34.0	29.0	35.0	2.0	35.0
116-117	27.618799543000524	34.0	28.0	35.0	2.0	35.0
118-119	27.51070020021438	34.0	27.5	35.0	2.0	35.0
120-121	27.34180621530784	34.0	27.0	35.0	2.0	35.0
122-123	27.19470140059748	34.0	27.0	35.0	2.0	35.0
124-125	26.17816924334401	33.0	23.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	108976.0
3	15436.0
4	9944.0
5	8950.0
6	10422.0
7	11381.0
8	11808.0
9	12981.0
10	14212.0
11	15671.0
12	16791.0
13	17027.0
14	17402.0
15	15316.0
16	12032.0
17	10691.0
18	9865.0
19	9350.0
20	9187.0
21	9933.0
22	11706.0
23	15473.0
24	21806.0
25	28484.0
26	32605.0
27	37104.0
28	43770.0
29	51584.0
30	60489.0
31	72729.0
32	89772.0
33	114005.0
34	150740.0
35	215956.0
36	363985.0
37	571364.0
38	206708.0
39	234.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.77301718889545	19.927197169061795	13.874070245981713	24.42571539606104
2	15.540117209536064	20.39120756040101	39.860192688732894	24.208482541330035
3	18.86085429768531	24.704771775031986	29.4580693798647	26.976304547418007
4	12.722431289944424	16.7527916186769	37.012911557167875	33.5118655342108
5	13.664885460140457	37.913956023427346	34.42239434393367	13.998764172498527
6	32.164450017202746	36.42403818598506	17.48196996458452	13.929541832227677
7	27.662170662050777	32.74690389892776	21.38912747511541	18.201797963906056
8	25.44172541832329	35.78193487146895	20.21902748969896	18.557312220508805
9	25.96767624681205	16.462894932611633	19.78337518249134	37.78605363808498
10-11	24.519072561234097	26.7986241766769	27.88715046297009	20.795152799118917
12-13	24.933668816019413	24.343570180907335	27.834278888423814	22.888482114649435
14-15	22.687293986314646	25.81869173958125	25.391971283460258	26.10204299064385
16-17	22.530202771044838	28.536863608845454	26.25241754580378	22.68051607430593
18-19	22.443750061583028	27.906657371734866	27.645196359539785	22.004396207142317
20-21	23.605981687221085	26.92882191292623	27.30214360762812	22.163052792224562
22-23	23.110790153573802	26.707029038491775	26.954281751908127	23.227899056026295
24-25	22.761571946269434	26.860233444440656	27.271969629478487	23.106224979811422
26-27	22.723150504967567	27.016072748173087	27.265477461203712	22.995299285655637
28-29	22.688767702024013	27.187270657621788	27.307732180450046	22.816229459904154
30-31	23.105508843381013	27.03356707667548	27.109725255776524	22.75119882416698
32-33	22.768995750702693	26.941378335665544	27.130206343709673	23.159419569922093
34-35	22.826702128161855	27.072515486862365	27.249056582858227	22.85172580211755
36-37	23.001518636864322	27.014143973672034	27.103459856298112	22.880877533165528
38-39	22.869782014849825	27.137012316830155	27.120200964425617	22.873004703894402
40-41	22.967407205790316	27.117460133698135	27.109085269866647	22.806047390644906
42-43	23.006846664880847	26.96044411464956	27.03302635724791	22.99968286322168
44-45	23.02610646953219	27.030752929285384	26.987810740719908	22.95532986046252
46-47	22.873294287757627	27.0428724034049	26.984472363335694	23.099360945501783
48-49	22.849902702170112	27.126758134837548	27.0494330451347	22.97390611785764
50-51	22.871890897812047	27.265468045750595	26.967718441569744	22.89492261486761
52-53	22.94070772045239	27.214274453518485	26.744052379458765	23.100965446570356
54-55	22.92735547148432	27.15686858132866	26.76729897999191	23.148476967195112
56-57	22.997009038160783	27.15923079465244	26.635160746494485	23.2085994206923
58-59	22.83852784839618	27.058539774552536	27.0126620474193	23.09027032963198
60-61	23.07505350466321	27.158322484603424	26.878061918022606	22.888562092710764
62-63	22.840624512484503	27.13845850678622	26.903301557586357	23.117615423142926
64-65	22.911064049296257	27.019256684703834	26.966050422376025	23.10362884362388
66-67	22.984960535466353	27.126889170058355	26.673086033750735	23.215064260724557
68-69	23.06004055279561	27.04598049869511	26.75998024498355	23.13399870352573
70-71	22.962876903001842	26.8389483694743	26.917319192374094	23.280855535149765
72-73	23.084192722017864	26.92377167630058	26.83094948765108	23.16108611403048
74-75	23.001951278389086	27.042242939701676	26.772520679732935	23.183285102176292
76-77	22.865274967039177	26.890282289955465	26.937226955803663	23.3072157872017
78-79	23.021424429494385	26.930554058838922	26.88184491278846	23.166176598878234
80-81	22.920368259952838	26.99758773802408	26.846841890924377	23.235202111098705
82-83	23.01573389252708	26.942679630590572	26.873505678421992	23.168080798460355
84-85	23.165242130154194	26.889604578222244	26.661370839286008	23.283782452337555
86-87	22.94998448201967	26.8174510773091	26.81981161664513	23.412752824026104
88-89	23.00737020447155	26.731944682208425	26.913500574123038	23.347184539196984
90-91	23.004469236818235	26.829255776518863	26.886791581421786	23.279483405241116
92-93	23.036785486146123	26.769647745429527	26.937843514302184	23.25572325412217
94-95	22.785986380985108	26.91520732521874	26.97362614174302	23.325180152053136
96-97	22.906561581305336	26.806927817309774	26.983456773600796	23.303053827784098
98-99	22.866544054255993	26.783661622589143	26.814307883047466	23.535486440107395
100-101	22.684983245154434	26.997500885210062	26.84478290543904	23.47273296419646
102-103	22.75127333471958	26.93793918578422	26.883070790903385	23.427716688592813
104-105	22.759588304362463	27.06607329069128	26.75559779618849	23.418740608757773
106-107	22.585609541909047	27.130903598456158	26.834976982344987	23.44850987728981
108-109	22.585148919126926	27.085928090423693	26.760587378759364	23.56833561169001
110-111	22.556188009666368	27.331193737062037	26.66659002768036	23.44602822559124
112-113	22.565071122997615	27.278687568906594	26.659675168336843	23.496566139758944
114-115	22.556889970470895	27.360845248811188	26.664442831879114	23.4178219488388
116-117	22.540186002345404	27.277730235223768	26.697024565964078	23.485059196466747
118-119	22.576399467211743	27.197797742854746	26.649429802027164	23.576372987906346
120-121	22.580460414252126	27.339810975041495	26.605092531580453	23.474636079125926
122-123	22.60774482720965	27.2935225223339	26.549394446586046	23.549338203870402
124-125	22.570573743954167	27.30246993723674	26.51977806885059	23.6071782499585
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	726.0
1	541.0
2	286.0
3	218.5
4	178.5
5	148.5
6	160.5
7	201.0
8	254.0
9	302.0
10	334.5
11	357.5
12	428.5
13	510.0
14	625.5
15	726.5
16	790.0
17	903.0
18	1091.0
19	1324.0
20	1608.0
21	2012.5
22	2589.0
23	3361.0
24	4371.0
25	5856.5
26	8189.0
27	11172.5
28	14550.0
29	18374.0
30	22172.5
31	27080.0
32	32925.0
33	39015.5
34	45067.0
35	50723.0
36	57523.0
37	63677.5
38	67124.5
39	69984.0
40	74723.5
41	78720.0
42	81406.0
43	85533.5
44	89232.0
45	92575.5
46	96195.5
47	98634.0
48	100182.0
49	101390.0
50	102583.0
51	99991.0
52	93704.5
53	88991.0
54	84407.0
55	78003.0
56	70681.5
57	62929.0
58	56518.0
59	50622.0
60	43307.0
61	35845.0
62	28691.0
63	21375.0
64	15594.5
65	11734.0
66	8642.5
67	6427.5
68	4789.5
69	3591.5
70	2753.5
71	2111.0
72	1653.5
73	1376.5
74	1083.5
75	783.5
76	588.5
77	388.0
78	246.5
79	167.0
80	113.5
81	95.0
82	85.0
83	71.0
84	48.5
85	30.0
86	22.5
87	21.0
88	15.5
89	11.5
90	11.5
91	9.0
92	5.0
93	5.0
94	9.0
95	9.0
96	6.5
97	4.5
98	6.5
99	7.0
100	7.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06744970727319677
2	0.010304246211547408
3	0.01991059526932467
4	0.019459014758061638
5	0.01124846000782466
6	0.009483190736523709
7	0.010057929569040298
8	0.008744240809002381
9	0.0064452854789360265
10-11	0.010530036467178924
12-13	0.00798476449460546
14-15	0.009011083838385083
16-17	0.012705833475991723
18-19	0.006281074383931287
20-21	0.013218993147881533
22-23	0.005008438397644556
24-25	0.004351594017625598
26-27	0.0036536968638554547
28-29	0.010530036467178924
30-31	0.006937918763950246
32-33	0.0030584316444632743
34-35	0.008025817268356645
36-37	0.006240021610180103
38-39	0.001436847081291471
40-41	0.0011700040519087693
42-43	0.001293162373162324
44-45	0.0022989553300663537
46-47	0.004228435696372043
48-49	0.002832641388831757
50-51	0.004823700915764224
52-53	0.005131596718898111
54-55	0.005090543945146926
56-57	0.0020731650744348364
58-59	0.0027300094544537947
60-61	0.004926332850142186
62-63	0.002832641388831757
64-65	0.0035100121557263076
66-67	0.0012726359862867316
68-69	0.002134744235061614
70-71	0.0013752679206646937
72-73	0.0020115859138080594
74-75	0.002052638687559244
76-77	0.0017447428844253577
78-79	6.157916062677733E-5
80-81	4.105277375118488E-5
82-83	4.105277375118488E-5
84-85	3.69474963760664E-4
86-87	2.0526386875592445E-4
88-89	0.0
90-91	5.952652193921808E-4
92-93	0.0015189526287938408
94-95	0.0012315832125355465
96-97	0.0011494776650331768
98-99	0.0011905304387843617
100-101	0.0010878985044063996
102-103	0.0024836928119466853
104-105	0.004474752338879152
106-107	0.006157916062677733
108-109	0.01184372522721684
110-111	0.009318979641518968
112-113	0.003017378870712089
114-115	0.006424759092060434
116-117	0.002442640038195501
118-119	0.0010057929569040297
120-121	0.005172649492649296
122-123	7.38949927521328E-4
124-125	0.08132554480109726
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2435889.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.51177719725232
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.43813195704597	38.942195326304
2	14.311249066741372	15.602632410811815
3	5.829751049104639	9.533702709127203
4	2.89668882844553	6.316146241039703
5	1.7093862527246748	4.659084127628676
6	1.006204596749219	3.291000047570673
7	0.6670503425830693	2.545346975796737
8	0.44385240048516705	1.9356146530970428
9	0.3223715687987112	1.5815742419775638
>10	1.3109426798236166	11.811584575685712
>50	0.04890265154359856	1.7766273433361748
>100	0.014106839956532679	1.2699216506442603
>500	9.83497665137057E-4	0.38151270305242857
>1k	3.7826833274446785E-4	0.3530569939280149
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	2.0526386875592444E-5	0.0	0.0	0.0	0.0
14-15	4.105277375118489E-5	0.0	0.0	0.0	2.0526386875592444E-5
16-17	6.157916062677733E-5	0.0	0.0	0.0	4.105277375118489E-5
18-19	8.210554750236978E-5	0.0	0.0	0.0	4.105277375118489E-5
20-21	8.210554750236978E-5	0.0	0.0	2.0526386875592444E-5	4.105277375118489E-5
22-23	8.210554750236978E-5	0.0	0.0	2.6684302938270174E-4	4.105277375118489E-5
24-25	8.210554750236978E-5	0.0	0.0	9.647401831528448E-4	4.105277375118489E-5
26-27	8.210554750236978E-5	0.0	0.0	0.0018679012056789122	4.105277375118489E-5
28-29	1.6421109500473953E-4	0.0	0.0	0.004474752338879152	4.105277375118489E-5
30-31	2.0526386875592442E-4	0.0	0.0	0.012233726577853096	4.105277375118489E-5
32-33	2.2579025563151687E-4	0.0	0.0	0.027669569508298617	8.210554750236978E-5
34-35	2.463166425071093E-4	0.0	0.0	0.04955069791768016	8.210554750236978E-5
36-37	2.463166425071093E-4	0.0	0.0	0.08263923356113517	8.210554750236978E-5
38-39	2.873694162582942E-4	0.0	0.0	0.1363773144014362	8.210554750236978E-5
40-41	2.873694162582942E-4	0.0	0.0	0.19890068882449077	8.210554750236978E-5
42-43	3.0789580313388663E-4	0.0	0.0	0.25549193744049914	8.210554750236978E-5
44-45	3.284221900094791E-4	0.0	0.0	0.3237216474149684	8.210554750236978E-5
46-47	3.284221900094791E-4	0.0	0.0	0.40642246013673033	8.210554750236978E-5
48-49	3.284221900094791E-4	0.0	0.0	0.4910938059985492	8.210554750236978E-5
50-51	3.284221900094791E-4	0.0	0.0	0.5779409488691809	8.210554750236978E-5
52-53	3.284221900094791E-4	0.0	0.0	0.6654244097329558	8.210554750236978E-5
54-55	3.284221900094791E-4	0.0	0.0	0.7660242318102344	8.210554750236978E-5
56-57	3.6947496376066395E-4	0.0	0.0	0.8840714827317665	8.210554750236978E-5
58-59	4.1052773751184884E-4	0.0	0.0	1.0074555942409527	8.210554750236978E-5
60-61	4.1052773751184884E-4	0.0	0.0	1.1332823457883343	8.210554750236978E-5
62-63	4.1052773751184884E-4	0.0	0.0	1.2679559700790963	8.210554750236978E-5
64-65	4.1052773751184884E-4	0.0	0.0	1.4152738486852234	8.210554750236978E-5
66-67	4.926332850142186E-4	0.0	0.0	1.5858070708476455	8.210554750236978E-5
68-69	4.926332850142186E-4	0.0	0.0	1.773644037146192	8.210554750236978E-5
70-71	4.926332850142186E-4	0.0	0.0	1.9830131832772346	1.2315832125355466E-4
72-73	4.926332850142186E-4	0.0	0.0	2.189426529698192	1.6421109500473955E-4
74-75	4.926332850142186E-4	0.0	0.0	2.411604141239605	1.6421109500473955E-4
76-77	4.926332850142186E-4	0.0	0.0	2.663380802655622	1.6421109500473955E-4
78-79	4.926332850142186E-4	0.0	0.0	2.942519137776803	1.6421109500473955E-4
80-81	6.773707668945506E-4	0.0	0.0	3.238899637873483	1.6421109500473955E-4
82-83	6.97897153770143E-4	2.0526386875592444E-5	0.0	3.5483554464099143	1.6421109500473955E-4
84-85	6.97897153770143E-4	4.105277375118489E-5	0.0	3.8691418205016728	1.6421109500473955E-4
86-87	6.97897153770143E-4	4.105277375118489E-5	0.0	4.2259109507863455	1.6421109500473955E-4
88-89	6.97897153770143E-4	4.105277375118489E-5	0.0	4.618375467847673	1.6421109500473955E-4
90-91	8.210554750236977E-4	4.105277375118489E-5	0.0	5.02662477641633	1.6421109500473955E-4
92-93	8.210554750236977E-4	4.105277375118489E-5	0.0	5.443782536888996	1.6421109500473955E-4
94-95	8.210554750236977E-4	4.105277375118489E-5	0.0	5.884874064458602	1.6421109500473955E-4
96-97	8.210554750236977E-4	4.105277375118489E-5	0.0	6.352834632448358	2.0526386875592442E-4
98-99	8.210554750236977E-4	4.105277375118489E-5	0.0	6.860636917363641	2.0526386875592442E-4
100-101	8.415818618992901E-4	4.105277375118489E-5	0.0	7.4014251059879985	2.0526386875592442E-4
102-103	8.82634635650475E-4	4.105277375118489E-5	0.0	7.9474680496525085	2.0526386875592442E-4
104-105	0.001067372117530807	4.105277375118489E-5	0.0	8.498170483137779	2.0526386875592442E-4
106-107	0.0010878985044063994	4.105277375118489E-5	0.0	9.07005614787866	2.0526386875592442E-4
108-109	0.001108424891281992	4.105277375118489E-5	0.0	9.685663016664552	2.0526386875592442E-4
110-111	0.001108424891281992	4.105277375118489E-5	0.0	10.325655233058649	2.0526386875592442E-4
112-113	0.001108424891281992	4.105277375118489E-5	0.0	10.981309082638823	2.0526386875592442E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7890	0.0	29.129255	1
GTACATG	11910	0.0	25.246355	1
TACATGG	11820	0.0	25.023775	2
CATGGGG	6545	0.0	24.822706	4
TCAACGC	9225	0.0	24.32042	4
AACGCAG	9215	0.0	24.214668	6
ATCAACG	9460	0.0	23.843061	3
CAACGCA	9360	0.0	23.842485	5
TATCAAC	9560	0.0	23.655909	2
GGTATCA	6185	0.0	23.585522	1
ACATGGG	12225	0.0	23.561907	3
ACGCAGA	10280	0.0	21.474516	7
CGCAGAG	10425	0.0	20.947517	8
GTATAGG	515	0.0	20.810587	1
ATGGGGC	2915	0.0	19.394613	5
GTATAAT	1675	0.0	18.839968	1
GTATACG	190	8.286499E-4	18.80255	1
GCAGAGT	11425	0.0	18.384514	9
GTATTCG	200	0.0011693763	17.862423	1
GAGTACT	3645	0.0	17.713385	12-13
>>END_MODULE