FastQCFastQC Report
Mon 15 Aug 2016
SRR2921111_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921111_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2435889
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34740.1426173360116163No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32420.1330930925013414No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA102800.032.554831
GGTATCA76200.032.0977481
TCAACGC123300.026.7474634
TATCAAC124500.026.5359172
ATCAACG124400.026.5093123
CAACGCA124100.026.475215
AACGCAG126850.025.9337886
CATGGGG62500.023.473384
GAGTACT65050.023.3678912-13
ACGCAGA140400.023.3047647
CGCAGAG144100.022.541248
GTACATG126000.021.8260971
TACATGG122500.021.8089942
AGTACTT72450.021.5969912-13
GTACTTT71950.021.29228814-15
ACATGGG125200.020.9099943
GCAGAGT153250.020.536729
AGAGTAC139100.019.6318610-11
CAGAGTA145450.018.30438810-11
ATGGGGG33550.017.5636525
Produced by FastQC (version 0.11.5)