FastQCFastQC Report
Mon 15 Aug 2016
SRR2921110_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921110_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2087717
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA47260.22637167777050243No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT42830.2051523266802924No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26970.12918417582459693No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23050.11040768456644268No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG21750.1041807869553201No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21420.10260011294634282No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG77450.028.1222784
ATGGGGG37400.024.5041685
TACATGG168850.023.8951112
GTACATG172750.023.8861061
GTATCAA127850.022.7273751
TACCGTA2653.7280188E-822.453967
ACATGGG174400.022.3157623
GTATAAT15050.020.9686071
ACCGTAT2306.1417522E-620.6961988
TATAATG15050.020.1657372
ATGGGGA24950.019.5584375
AACGCAG142700.019.5563456
TATCAAC146150.019.4222722
GTACCGT3402.4436304E-819.2509696
TCAACGC143750.019.250234
GTAAGTA11750.019.2453652
GGTATCA103000.019.0190531
ATCAACG146200.019.0085933
CATGGGT16600.019.000224
CAACGCA146550.018.963655
Produced by FastQC (version 0.11.5)