##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921110_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2087717
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.372903990339687	31.0	30.0	34.0	23.0	34.0
2	29.41180820963761	31.0	30.0	34.0	23.0	34.0
3	29.517408250256143	31.0	30.0	34.0	23.0	34.0
4	32.9142067626982	37.0	35.0	37.0	25.0	37.0
5	33.05753844989527	37.0	35.0	37.0	26.0	37.0
6	33.02629714659602	37.0	35.0	37.0	26.0	37.0
7	32.96844304089108	37.0	35.0	37.0	26.0	37.0
8	32.87091880748205	37.0	35.0	37.0	25.0	37.0
9	34.21073354290835	39.0	35.0	39.0	25.0	39.0
10-11	34.17776427552202	38.5	35.0	39.0	25.0	39.0
12-13	34.13762952545771	39.0	35.0	39.0	25.0	39.0
14-15	35.15942390659271	39.0	35.0	41.0	24.5	41.0
16-17	35.07860045207276	39.0	34.5	41.0	24.0	41.0
18-19	35.17074320896941	39.5	35.0	41.0	24.5	41.0
20-21	35.2204223560952	40.0	35.0	41.0	25.0	41.0
22-23	35.21561279617879	39.0	35.0	41.0	25.0	41.0
24-25	35.13895154371977	39.0	34.5	41.0	24.5	41.0
26-27	35.07098423780618	39.0	34.5	41.0	24.5	41.0
28-29	34.92001286572845	39.0	34.0	41.0	24.0	41.0
30-31	34.79338219691654	39.0	34.0	41.0	24.0	41.0
32-33	34.65055824137084	39.0	34.0	41.0	23.5	41.0
34-35	34.5181880494339	39.0	34.0	41.0	22.5	41.0
36-37	34.36330139573515	39.0	34.0	41.0	21.5	41.0
38-39	34.17998775696131	39.0	33.0	41.0	19.0	41.0
40-41	34.049357264418504	39.0	33.0	41.0	18.0	41.0
42-43	33.94091704000111	38.5	33.0	41.0	17.0	41.0
44-45	33.76080977450488	38.0	33.0	41.0	15.5	41.0
46-47	33.49338248431181	38.0	33.0	40.0	12.5	41.0
48-49	33.30972756364967	38.0	32.5	40.0	9.0	41.0
50-51	33.13691031878363	38.0	32.0	40.0	8.0	41.0
52-53	32.9294636198297	38.0	32.0	40.0	6.5	41.0
54-55	32.678647536998554	38.0	32.0	40.0	2.0	41.0
56-57	32.40228488822959	37.0	31.0	40.0	2.0	41.0
58-59	32.1637719575977	37.0	31.0	40.0	2.0	41.0
60-61	31.84757560531432	36.0	31.0	40.0	2.0	41.0
62-63	31.556554839568776	36.0	30.5	39.0	2.0	41.0
64-65	31.248123668102526	35.5	30.0	39.0	2.0	41.0
66-67	30.952033249717275	35.0	30.0	39.0	2.0	40.0
68-69	30.594597112539677	35.0	29.5	38.0	2.0	40.0
70-71	30.269156691256526	35.0	29.0	37.5	2.0	40.0
72-73	29.91219667225012	35.0	29.0	37.0	2.0	39.0
74-75	29.840567711045125	35.0	29.0	36.5	2.0	39.0
76-77	29.594871575026694	35.0	29.0	36.0	2.0	39.0
78-79	29.34052771520278	34.0	29.0	36.0	2.0	38.5
80-81	29.0086771818211	34.0	29.0	35.0	2.0	37.0
82-83	28.76449873234734	34.0	29.0	35.0	2.0	37.0
84-85	28.573814841762555	34.0	29.0	35.0	2.0	36.0
86-87	28.361410334829863	34.0	29.0	35.0	2.0	36.0
88-89	28.19679535109404	34.0	28.5	35.0	2.0	36.0
90-91	28.037127158518132	34.0	28.0	35.0	2.0	35.0
92-93	28.195677862468905	34.0	29.0	35.0	2.0	35.0
94-95	28.167433852385166	34.0	29.0	35.0	2.0	35.0
96-97	28.099815971225986	34.0	29.0	35.0	2.0	35.0
98-99	27.99543592354711	34.0	29.0	35.0	2.0	35.0
100-101	27.881077990934592	34.0	28.5	35.0	2.0	35.0
102-103	26.58462521500759	32.0	25.0	34.0	2.0	35.0
104-105	27.560605915456932	34.0	27.0	35.0	2.0	35.0
106-107	27.704850801138278	34.0	27.5	35.0	2.0	35.0
108-109	27.63898627064875	34.0	27.0	35.0	2.0	35.0
110-111	27.61094942465861	34.0	27.0	35.0	2.0	35.0
112-113	27.52074658586389	34.0	27.0	35.0	2.0	35.0
114-115	27.373814554367282	34.0	27.0	35.0	2.0	35.0
116-117	27.202823945965857	34.0	27.0	35.0	2.0	35.0
118-119	27.07039148505281	34.0	27.0	35.0	2.0	35.0
120-121	26.871926846406865	34.0	25.0	35.0	2.0	35.0
122-123	26.693321221219158	34.0	25.0	35.0	2.0	35.0
124-125	25.621355289054982	33.0	21.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	88102.0
3	12430.0
4	8486.0
5	8820.0
6	12259.0
7	12405.0
8	11306.0
9	12020.0
10	13092.0
11	14278.0
12	14755.0
13	15418.0
14	16723.0
15	14699.0
16	10943.0
17	9764.0
18	8930.0
19	8395.0
20	8587.0
21	8965.0
22	10553.0
23	14257.0
24	19926.0
25	25533.0
26	28820.0
27	32071.0
28	37738.0
29	44682.0
30	52748.0
31	63982.0
32	79084.0
33	100484.0
34	135265.0
35	193233.0
36	322131.0
37	464774.0
38	151857.0
39	202.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.88053448981607	18.839625255113535	13.28694556842986	23.992894686640533
2	15.753825606751004	20.64026458291018	39.03354807062187	24.572361739716943
3	19.31273762801251	25.104489253081496	28.906058364506038	26.67671475439995
4	12.978273169465062	16.851983421859632	35.89049535602232	34.27924805265299
5	13.633221507214246	38.65795243709242	33.42654039560865	14.282285660084687
6	32.06451628355932	36.00897709499481	17.23650142392822	14.690005197517648
7	27.74814660103204	33.56261700509122	20.440034950630317	18.249201443246424
8	26.025605159737648	35.54126659602558	19.765485631947147	18.667642612289633
9	26.575413334482985	16.91242793741123	19.137093162227348	37.375065565878444
10-11	24.869263128597467	27.16423923512134	26.639207911685514	21.327289724595683
12-13	25.20998863023151	24.888823208602798	26.85413810951432	23.04705005165137
14-15	23.34066014080213	25.85769035747587	24.709616273129203	26.092033228592797
16-17	22.702484022455987	28.676799231219167	25.711091129367208	22.909625616957634
18-19	22.760738958778227	27.79816017160491	27.38380598451992	22.057294885096947
20-21	23.93303673441622	26.665925728271382	27.164238736099623	22.236798801212775
22-23	23.669858982754022	26.21793173747867	26.644829966416257	23.46737931335106
24-25	23.17844377233984	26.589356009039882	26.786516627882804	23.445683590737474
26-27	22.843846961711687	26.776790621980002	26.876639854189943	23.502722562118368
28-29	22.890126253861975	26.944927677861635	27.100616263325445	23.064329804950944
30-31	23.57405241605673	26.784675406109116	26.75356290796233	22.88770926987182
32-33	23.045865161187415	26.819127416637645	26.72687114730682	23.40813627486812
34-35	22.941420915541453	27.05432771473908	27.00939492829556	22.99485644142391
36-37	23.37938645969106	26.881057526170142	26.751601967628684	22.98795404651011
38-39	23.32955403840894	26.896245660876062	26.644723764196904	23.129476536518094
40-41	23.169342029379813	27.034303910730127	26.672157578799936	23.124196481090124
42-43	23.20919252391332	26.786197131623297	26.611339339793883	23.3932710046695
44-45	23.16289625596625	26.940076826853137	26.48239206986315	23.414634847317465
46-47	23.083573935671456	26.938664067243312	26.58098788465937	23.39677411242586
48-49	23.10058582055593	27.092620002203414	26.639842503843997	23.166951673396657
50-51	22.916598806296165	27.20277157720275	26.440755501480158	23.439874115020935
52-53	23.077588170443978	27.049290854626935	26.125582811227243	23.747538163701844
54-55	22.906438215183268	27.01329096931171	26.214989745502116	23.865281070002904
56-57	23.213786186031985	26.90828688632954	25.955939692022355	23.92198723561612
58-59	23.120130940109366	26.772883862982304	26.331938158867867	23.77504703804046
60-61	23.418345449180503	26.7389146704579	26.336950523657514	23.50578935670408
62-63	22.882006041711424	26.61291732560025	26.529091154255248	23.97598547843308
64-65	22.830083568128188	26.511229437287497	26.535155999268074	24.12353099531624
66-67	23.09561507859051	26.76440953715078	26.18185136336192	23.958124020896797
68-69	23.258287516441868	26.398959982909076	26.21401589723987	24.12873660340918
70-71	22.886032669663926	26.301560675312285	26.5693930983874	24.24301355663639
72-73	23.08862913180535	26.498567546608026	26.317552954791378	24.095250366795245
74-75	23.04469524618586	26.502321617906745	26.488837624699997	23.964145511207402
76-77	22.811209237470848	26.071253564378676	26.80396001094042	24.313577187210054
78-79	23.092306188999974	26.316637799228243	26.689272042396816	23.901783969374968
80-81	22.95522012729243	26.40737170467677	26.4460743517127	24.1913338163181
82-83	23.015180947787186	26.238105718906624	26.59885889624329	24.147854437062904
84-85	23.177244869366124	26.318900419360013	26.12397345410391	24.379881257169956
86-87	22.908449928390436	26.299198643489756	26.415689918618966	24.376661509500842
88-89	22.909977741236002	26.002039548463706	26.706876458830386	24.38110625146991
90-91	23.161550742277257	25.988852788503316	26.521449233293914	24.32814723592552
92-93	23.10104261743367	26.00668637436272	26.53356347732661	24.358707530877
94-95	22.73267127544538	26.27693626784724	26.581221042950737	24.409171413756642
96-97	22.720504710102322	26.087186648806448	26.702578707075535	24.489729934015696
98-99	22.625931535549952	26.07775034703561	26.53562659447176	24.760691522942672
100-101	22.49782235024272	26.456366892251303	26.49641121883003	24.549399538675946
102-103	22.48569630933765	26.4535589000223	26.46210914083013	24.598635649809918
104-105	22.546853006498548	26.439875657417456	26.435133413919537	24.578137922164455
106-107	22.280937190206558	26.53397078579724	26.653127867404397	24.53196415659181
108-109	22.310769282359225	26.58722421693298	26.572397621037958	24.52960887966984
110-111	22.130501817122703	26.97911644219998	26.36457855040722	24.5258031902701
112-113	21.99258492843594	27.03845011592037	26.287913624954495	24.6810513306892
114-115	22.048154246878838	27.05784856740816	26.255716894703752	24.638280291009252
116-117	21.99690371334057	26.835881478898404	26.321334583855048	24.84588022390598
118-119	21.947666168429418	26.5450124742928	26.523529435714583	24.9837919215632
120-121	22.041324669539165	26.80606816869969	26.455500655053733	24.697106506707414
122-123	22.016961732279956	26.68827577915308	26.261633918099804	25.033128570467156
124-125	21.767404311581746	26.703445842519734	26.316238591000896	25.21291125489762
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1660.0
1	1146.5
2	550.5
3	405.5
4	298.5
5	238.0
6	253.5
7	322.0
8	407.0
9	476.5
10	488.5
11	510.0
12	571.0
13	646.0
14	754.0
15	866.0
16	997.5
17	1164.0
18	1342.0
19	1558.5
20	1858.5
21	2263.0
22	2798.0
23	3491.0
24	4383.5
25	5681.5
26	7936.5
27	10768.0
28	13268.0
29	16353.5
30	19773.5
31	23393.0
32	27199.5
33	30668.0
34	34685.0
35	38871.0
36	43315.0
37	46677.5
38	47686.5
39	49113.5
40	52765.0
41	55843.5
42	56870.0
43	60124.0
44	65730.0
45	69874.0
46	73089.5
47	77325.5
48	81968.0
49	88022.0
50	95331.0
51	94934.5
52	88462.0
53	85037.5
54	82201.0
55	76915.5
56	70350.0
57	63894.5
58	58437.5
59	52932.0
60	45416.0
61	37319.0
62	30053.5
63	22325.5
64	15772.5
65	11489.5
66	8136.5
67	5673.5
68	4121.0
69	3179.5
70	2498.5
71	1890.0
72	1452.5
73	1201.0
74	943.5
75	675.5
76	445.0
77	296.0
78	192.5
79	105.0
80	73.5
81	62.0
82	45.0
83	30.0
84	24.0
85	20.0
86	17.0
87	16.0
88	9.5
89	7.5
90	11.5
91	10.0
92	7.0
93	8.5
94	9.0
95	7.5
96	5.0
97	3.5
98	5.0
99	11.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06662780443901162
2	0.009819338540616377
3	0.02016556841755851
4	0.01868069283336774
5	0.011974803098312654
6	0.008717656655571613
7	0.009627741691043374
8	0.008094966894459354
9	0.005843703911976575
10-11	0.010657574757498262
12-13	0.007831521226296476
14-15	0.008502110199801984
16-17	0.012262198372672159
18-19	0.006274796823515831
20-21	0.013124384195750669
22-23	0.004789921239325062
24-25	0.003784037779066799
26-27	0.003328995261330918
28-29	0.010370179483138758
30-31	0.006298746429712457
32-33	0.0025865574692355334
34-35	0.007400428314757221
36-37	0.0061310991863360795
38-39	0.001245379522224516
40-41	0.0011974803098312656
42-43	9.819338540616375E-4
44-45	0.0017004220399603968
46-47	0.003568491323297171
48-49	0.002251262982482779
50-51	0.00464622360214531
52-53	0.004718072420735186
54-55	0.004622273995948684
56-57	0.0015806740089772703
58-59	0.0021554645576962778
60-61	0.004454626752572307
62-63	0.003041599986971414
64-65	0.003784037779066799
66-67	0.0013651275532076427
68-69	0.0028260535312017864
70-71	0.0014848755841907692
72-73	0.0019159684957300246
74-75	0.00270630550021866
76-77	0.0022033637700895285
78-79	6.22689761112258E-4
80-81	2.1554645576962777E-4
82-83	7.184881858987592E-5
84-85	5.747905487190073E-4
86-87	3.352944867527543E-4
88-89	0.0
90-91	9.579842478650123E-4
92-93	0.0018441196771401488
94-95	0.0016525228275671463
96-97	0.0011256314912413896
98-99	0.0016764724337637715
100-101	0.0014609259779941439
102-103	0.00270630550021866
104-105	0.004670173208341935
106-107	0.0065382424916787096
108-109	0.012070601523099155
110-111	0.010226481845959007
112-113	0.0036882393542802976
114-115	0.007400428314757221
116-117	0.002634456681628784
118-119	0.0011256314912413896
120-121	0.004885719664111563
122-123	8.382362168818857E-4
124-125	0.08401521853776159
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2087717.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.45508424962987
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.43179681248007	27.279590211528042
2	13.210671389692646	10.688776481283819
3	6.212218024775378	7.539474107080715
4	3.482812304840806	5.6358986087192955
5	2.2445493548488247	4.540171662643079
6	1.5413106145290842	3.741231045937374
7	1.078796716148841	3.0549968428017786
8	0.7735539613608792	2.503535254279144
9	0.6200414121049042	2.2575444808467036
>10	3.180443393833374	22.342490409722902
>50	0.16343810865890138	4.510567732847039
>100	0.0563049216538985	3.8483141881306318
>500	0.002270491658084137	0.6078730307188521
>1k	0.0017924934142769472	1.449535943460636
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	4726	0.22637167777050243	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	4283	0.2051523266802924	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2697	0.12918417582459693	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2305	0.11040768456644268	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	2175	0.1041807869553201	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2142	0.10260011294634282	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	4.789921239325062E-5	0.0
12-13	0.0	0.0	0.0	4.789921239325062E-5	0.0
14-15	0.0	0.0	0.0	7.184881858987593E-5	0.0
16-17	0.0	0.0	0.0	1.9159684957300248E-4	0.0
18-19	0.0	0.0	0.0	2.3949606196625308E-4	0.0
20-21	0.0	0.0	0.0	3.8319369914600497E-4	0.0
22-23	0.0	0.0	0.0	8.142866106852605E-4	0.0
24-25	0.0	0.0	0.0	0.002083615739106402	0.0
26-27	0.0	0.0	0.0	0.004095382659622927	0.0
28-29	0.0	0.0	0.0	0.00919664877950412	0.0
30-31	0.0	0.0	0.0	0.022680277068204167	0.0
32-33	0.0	0.0	0.0	0.048450053335773	0.0
34-35	0.0	0.0	0.0	0.08353622641382907	0.0
36-37	0.0	0.0	0.0	0.13924301042717954	0.0
38-39	0.0	0.0	0.0	0.22725781319977756	0.0
40-41	0.0	0.0	0.0	0.3295944804779575	0.0
42-43	0.0	0.0	0.0	0.4158849116043985	0.0
44-45	0.0	0.0	0.0	0.5130005647317142	0.0
46-47	0.0	0.0	0.0	0.6356943972770256	0.0
48-49	0.0	0.0	0.0	0.7607352912296064	0.0
50-51	0.0	0.0	0.0	0.8894165253240741	0.0
52-53	0.0	0.0	0.0	1.0218338979852155	0.0
54-55	0.0	0.0	0.0	1.1678785965722365	0.0
56-57	0.0	0.0	0.0	1.3353342430990407	0.0
58-59	0.0	0.0	0.0	1.5205125982113477	0.0
60-61	0.0	0.0	0.0	1.7116544052666143	0.0
62-63	0.0	0.0	0.0	1.9074424359240263	0.0
64-65	0.0	0.0	0.0	2.116546447626762	0.0
66-67	0.0	0.0	0.0	2.364688317429996	0.0
68-69	0.0	0.0	0.0	2.648562999678596	0.0
70-71	0.0	0.0	0.0	2.9658713321776853	0.0
72-73	0.0	0.0	0.0	3.2753481434504774	0.0
74-75	0.0	0.0	0.0	3.5865972255818197	0.0
76-77	0.0	0.0	0.0	3.941961482327346	0.0
78-79	0.0	0.0	0.0	4.3354056129255065	0.0
80-81	0.0	0.0	0.0	4.76218759534937	0.0
82-83	0.0	0.0	0.0	5.211362459567077	0.0
84-85	0.0	0.0	0.0	5.664752454475391	0.0
86-87	0.0	0.0	0.0	6.1533244208865465	0.0
88-89	0.0	0.0	0.0	6.703183429554867	0.0
90-91	0.0	0.0	0.0	7.277758431818105	0.0
92-93	0.0	2.394960619662531E-5	0.0	7.864308237179657	0.0
94-95	0.0	4.789921239325062E-5	0.0	8.464629066104266	0.0
96-97	0.0	4.789921239325062E-5	0.0	9.086025548481906	0.0
98-99	0.0	4.789921239325062E-5	0.0	9.767942685718419	0.0
100-101	0.0	4.789921239325062E-5	0.0	10.514140565986674	0.0
102-103	0.0	4.789921239325062E-5	0.0	11.258398528153002	0.0
104-105	0.0	7.184881858987593E-5	0.0	12.012523728072338	0.0
106-107	0.0	9.579842478650124E-5	0.0	12.795460304246216	0.0
108-109	0.0	9.579842478650124E-5	0.0	13.604669598417793	0.0
110-111	0.0	9.579842478650124E-5	0.0	14.447408341264644	0.0
112-113	0.0	9.579842478650124E-5	0.0	15.318096274542958	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGGGG	7745	0.0	28.122278	4
ATGGGGG	3740	0.0	24.504168	5
TACATGG	16885	0.0	23.895111	2
GTACATG	17275	0.0	23.886106	1
GTATCAA	12785	0.0	22.727375	1
TACCGTA	265	3.7280188E-8	22.45396	7
ACATGGG	17440	0.0	22.315762	3
GTATAAT	1505	0.0	20.968607	1
ACCGTAT	230	6.1417522E-6	20.696198	8
TATAATG	1505	0.0	20.165737	2
ATGGGGA	2495	0.0	19.558437	5
AACGCAG	14270	0.0	19.556345	6
TATCAAC	14615	0.0	19.422272	2
GTACCGT	340	2.4436304E-8	19.250969	6
TCAACGC	14375	0.0	19.25023	4
GTAAGTA	1175	0.0	19.245365	2
GGTATCA	10300	0.0	19.019053	1
ATCAACG	14620	0.0	19.008593	3
CATGGGT	1660	0.0	19.00022	4
CAACGCA	14655	0.0	18.96365	5
>>END_MODULE