FastQCFastQC Report
Mon 15 Aug 2016
SRR2921110_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921110_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2087717
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53170.25468011229491355No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49820.23863387614317458No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT44300.21219351090210026No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA41560.19906912670634958No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30340.14532621040112237No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA151200.028.6857361
GGTATCA114700.028.087541
TATCAAC177100.024.0782432
CATGGGG76400.023.8120044
ATCAACG179400.023.6382753
TCAACGC178950.023.5988624
CAACGCA179500.023.4460185
GAGTACT84500.023.44452712-13
AACGCAG179900.023.3798756
AGTACTT84250.023.0551112-13
GTACTTT87900.022.47081214-15
GGGTATA4550.022.2905561
GTAAGTA12550.021.3848442
ACGCAGA199650.020.8882797
GTAGCCC12050.020.7888683
GTACATG170300.020.7391241
ATGGGGG41350.020.7300855
TACATGG166900.020.7256892
CGCAGAG201650.020.6806098
GTGTAGC14900.020.4205421
Produced by FastQC (version 0.11.5)