##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921110_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2087717
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.330580725261136	31.0	30.0	34.0	26.0	34.0
2	30.390883438703618	31.0	30.0	34.0	26.0	34.0
3	30.4690209448886	31.0	30.0	34.0	26.0	34.0
4	33.967037199007336	37.0	35.0	37.0	30.0	37.0
5	33.60938144394092	37.0	35.0	37.0	28.0	37.0
6	33.4533195830661	37.0	35.0	37.0	28.0	37.0
7	33.26964478423081	37.0	35.0	37.0	27.0	37.0
8	33.24339649483144	37.0	35.0	37.0	27.0	37.0
9	34.575452994826406	39.0	35.0	39.0	27.0	39.0
10-11	34.54389747269386	38.0	35.0	39.0	27.0	39.0
12-13	34.399213830226984	38.0	35.0	39.0	26.5	39.0
14-15	35.412267563084455	39.0	35.0	41.0	26.0	41.0
16-17	35.352083400192654	39.0	34.5	41.0	26.0	41.0
18-19	35.43295571190923	39.0	35.0	41.0	26.0	41.0
20-21	35.45675251961832	39.0	35.0	41.0	26.0	41.0
22-23	35.441337116093806	39.0	35.0	41.0	26.0	41.0
24-25	35.34736652525223	39.0	35.0	41.0	25.5	41.0
26-27	35.21633152386076	39.0	34.5	41.0	25.0	41.0
28-29	35.10393051357056	39.0	34.0	41.0	25.0	41.0
30-31	34.96267094630163	39.0	34.0	41.0	24.0	41.0
32-33	34.75676540450645	39.0	34.0	41.0	24.0	41.0
34-35	34.597470346795085	39.0	34.0	41.0	23.5	41.0
36-37	34.42831236225983	39.0	34.0	41.0	22.5	41.0
38-39	34.229903286700264	38.0	33.0	41.0	20.5	41.0
40-41	34.053642088463135	38.0	33.0	41.0	18.5	41.0
42-43	33.87914932914758	38.0	33.0	41.0	17.0	41.0
44-45	33.637997630904955	38.0	33.0	41.0	14.5	41.0
46-47	33.316960584217114	38.0	33.0	40.0	9.0	41.0
48-49	33.02201375952775	38.0	32.0	40.0	6.5	41.0
50-51	32.78007771168219	38.0	32.0	40.0	2.0	41.0
52-53	32.599513248203664	38.0	32.0	40.0	2.0	41.0
54-55	32.38491831028823	37.5	31.5	40.0	2.0	41.0
56-57	32.10761228653117	37.0	31.0	40.0	2.0	41.0
58-59	31.878399227481502	37.0	31.0	40.0	2.0	41.0
60-61	31.60203969216134	36.0	31.0	40.0	2.0	41.0
62-63	31.290618412361447	36.0	30.0	39.0	2.0	41.0
64-65	31.012991224385296	35.0	30.0	39.0	2.0	41.0
66-67	30.68593827611693	35.0	29.5	39.0	2.0	40.5
68-69	30.321337614245607	35.0	29.0	38.0	2.0	40.0
70-71	29.966113462696335	35.0	29.0	37.5	2.0	40.0
72-73	29.776057770282083	35.0	29.0	37.0	2.0	39.0
74-75	29.582583990071452	35.0	29.0	36.5	2.0	39.0
76-77	29.359797089356462	35.0	29.0	36.0	2.0	39.0
78-79	29.049536646968914	34.0	29.0	36.0	2.0	37.5
80-81	28.769095859256787	34.0	29.0	35.0	2.0	37.0
82-83	28.555228031385482	34.0	29.0	35.0	2.0	37.0
84-85	28.402245131883298	34.0	29.0	35.0	2.0	36.0
86-87	28.197499230020163	34.0	29.0	35.0	2.0	36.0
88-89	28.050127723249847	34.0	28.0	35.0	2.0	36.0
90-91	27.892436091673346	34.0	27.5	35.0	2.0	35.0
92-93	27.765205245730144	34.0	27.0	35.0	2.0	35.0
94-95	27.659533835285146	34.0	27.0	35.0	2.0	35.0
96-97	27.591472407419204	34.0	27.0	35.0	2.0	35.0
98-99	27.552137813697932	34.0	27.0	35.0	2.0	35.0
100-101	27.400353112993763	34.0	27.0	35.0	2.0	35.0
102-103	26.060794398857702	31.5	24.5	34.0	2.0	35.0
104-105	27.107119164139583	33.5	25.5	35.0	2.0	35.0
106-107	27.373715881989753	34.0	27.0	35.0	2.0	35.0
108-109	27.572088554147903	34.0	27.0	35.0	2.0	35.0
110-111	27.549893496101244	34.0	27.0	35.0	2.0	35.0
112-113	27.472440469661358	34.0	27.0	35.0	2.0	35.0
114-115	27.34577842686533	34.0	27.0	35.0	2.0	35.0
116-117	27.237305151991386	34.0	27.0	35.0	2.0	35.0
118-119	27.096424706988543	34.0	27.0	35.0	2.0	35.0
120-121	26.919095835307182	34.0	26.0	35.0	2.0	35.0
122-123	26.71816151327024	34.0	25.0	35.0	2.0	35.0
124-125	25.915389873244315	33.5	22.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	62824.0
3	19992.0
4	10121.0
5	8240.0
6	10076.0
7	12071.0
8	13371.0
9	14343.0
10	15274.0
11	16989.0
12	18334.0
13	20746.0
14	24240.0
15	19764.0
16	12760.0
17	11048.0
18	10234.0
19	9809.0
20	9283.0
21	9501.0
22	10337.0
23	12887.0
24	17341.0
25	23117.0
26	27741.0
27	31993.0
28	38529.0
29	45266.0
30	53956.0
31	65143.0
32	79666.0
33	100049.0
34	132783.0
35	188975.0
36	317234.0
37	463966.0
38	149413.0
39	301.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.84171461769877	19.234803149908252	14.01831886014277	23.905163372250204
2	16.11026036624766	20.82882081971995	38.25607255403401	24.80484625999838
3	19.080519231690378	25.081716721751206	29.825501748335487	26.012262298222925
4	13.334030748323084	17.21687270800802	35.58160091290982	33.86749563075907
5	13.390055298403839	38.23464986385099	33.877641796524685	14.497653041220484
6	32.01718794161465	36.22320372117978	16.85898510665817	14.9006232305474
7	27.42670610294632	33.389575063547404	20.57541901047131	18.608299823034965
8	26.64332759916589	34.98072083802198	19.53401386451748	18.841937698294647
9	26.843260847381867	15.642871070310782	19.316002562103954	38.19786552020339
10-11	25.176396246717125	26.382616969108142	26.79332990314016	21.647656881034578
12-13	26.031250905761734	23.86705226266168	27.325822467586008	22.775874363990578
14-15	23.769802778923	24.912902628456497	25.470498288236904	25.846796304383602
16-17	22.713204070397815	27.89870192466631	26.349640445637558	23.038453559298322
18-19	22.624110501975732	26.5963412749841	28.91683964541054	21.86270857762963
20-21	23.648196233469957	25.394575800021705	28.915986491604684	22.041241474903657
22-23	23.330669361303123	25.149021924585636	28.40639851584177	23.113910198269473
24-25	22.90474260312788	25.64982852346196	28.51062157969227	22.934807293717892
26-27	22.598996061190725	25.85405947453477	28.64600975766812	22.90093470660638
28-29	22.580023074549576	25.865295609127863	28.902813791834596	22.65186752448796
30-31	23.321601159099206	25.623078109832697	28.512872651449015	22.542448079619078
32-33	22.831948716474546	25.67082051724303	28.496660629928805	23.00057013635362
34-35	22.703606549522682	25.944254794159573	28.729263238588043	22.622875417729706
36-37	23.14371951077818	25.741401808864456	28.53080850295181	22.584070177405557
38-39	23.16826203775465	25.69968531799413	28.31254020371078	22.819512440540436
40-41	22.965024283468196	25.836493789646447	28.313136837636755	22.885345089248606
42-43	23.21360423685428	25.55434788077032	28.11204176867153	23.120006113703873
44-45	23.106308519802944	25.748521642804366	27.888390380209227	23.25677945718346
46-47	23.180652141206863	25.8653659106873	27.626139503060237	23.327842445045597
48-49	23.278283473519267	26.115165114843386	27.46948796898765	23.137063442649694
50-51	23.069129575339673	26.307440976941315	27.229558353930646	23.39387109378837
52-53	23.21175105078379	26.267043419619977	26.9216496796442	23.59955584995203
54-55	22.992827947645956	26.26082294251793	26.946476609794782	23.799872500041324
56-57	23.23432106041114	26.295556306192996	26.596498036763666	23.873624596632194
58-59	23.055779474102174	26.1830664876383	27.06882570725987	23.692328330999658
60-61	23.467734787306167	26.294237850087796	26.941770017080668	23.29625734552537
62-63	22.87056277906692	26.26675240011327	27.09831732139837	23.764367499421443
64-65	22.73858640821917	26.154967394187135	27.216504082824205	23.88994211476949
66-67	23.078707008987994	26.448845858612767	26.801192713389423	23.671254419009816
68-69	23.19532960839785	26.07256796553888	26.841934918958337	23.890167507104938
70-71	22.909006312447335	26.023757150828608	27.037625272023185	24.029611264700872
72-73	23.15194525773048	26.173000713987932	26.7751225519558	23.89993147632579
74-75	23.070440275980097	26.23530942115903	26.84627747637422	23.847972826486654
76-77	22.821285737007816	25.838702228490725	27.113200008431814	24.226812026069645
78-79	23.06638837259051	26.06223384415931	26.96045328748652	23.910924495763666
80-81	22.859766372312325	26.23721532584599	26.70172056189083	24.201297739950853
82-83	22.785449952951232	26.131637852889718	26.83339274318272	24.249519450976333
84-85	22.983591635327087	26.201355786044505	26.329508707212497	24.48554387141591
86-87	22.750935939776	26.17021516135005	26.581120808991155	24.497728089882795
88-89	22.708216258323404	25.944205463708066	26.82662167153651	24.520956606432023
90-91	22.82431303015546	26.000161452555588	26.659290313014345	24.51623520427461
92-93	22.824098927689274	26.007996755495284	26.54339752632866	24.624506790486777
94-95	22.55282524736739	26.25480248575872	26.549652774201306	24.64271949267258
96-97	22.609204744688075	26.029165980959252	26.657908775641566	24.70372049871111
98-99	22.583611599507506	25.97488178678024	26.40200639093962	25.03950022277263
100-101	22.414024383232984	26.347962187208957	26.38691135495196	24.851102074606104
102-103	22.50168991669465	26.277018905508864	26.257496834550086	24.96379434324639
104-105	22.496521849111488	26.280112833897697	26.21826307085271	25.0051022461381
106-107	22.25344690473225	26.323094022171333	26.482959499468233	24.940499573628188
108-109	22.3135509221311	26.395412842718162	26.35035664103501	24.94067959411572
110-111	22.088587553516277	26.739647291981743	26.129620412283128	25.042144742218852
112-113	21.93028007252712	26.846675081856485	26.02411023738979	25.198934608226608
114-115	21.98510435216866	26.85005790356751	25.95176818453454	25.213069559729295
116-117	21.944781700917527	26.661132174171726	25.984202744459978	25.409883380450772
118-119	21.85833459076114	26.439036307329726	26.221297713397473	25.481331388511663
120-121	21.914829154147473	26.81898470871839	26.104178068165893	25.162008068968245
122-123	21.768657461775156	26.816250345201396	25.783939494608394	25.631152698415054
124-125	21.47172520485891	26.93589154962383	25.84917361511159	25.743209630405673
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	115.0
1	112.5
2	190.0
3	291.5
4	239.0
5	186.5
6	311.5
7	665.0
8	1094.5
9	1302.0
10	1094.5
11	779.0
12	746.0
13	859.5
14	986.0
15	1128.5
16	1342.5
17	1545.0
18	1758.5
19	2049.0
20	2422.0
21	2837.5
22	3387.5
23	4124.0
24	5024.0
25	6295.0
26	8549.5
27	11318.0
28	13637.5
29	16662.5
30	20106.0
31	23731.0
32	27394.0
33	30910.5
34	35048.0
35	38801.0
36	42947.0
37	46429.0
38	47432.5
39	48512.5
40	52186.5
41	55248.5
42	56511.0
43	60184.0
44	65297.0
45	69370.5
46	73070.5
47	77658.5
48	82183.5
49	88535.0
50	97354.0
51	96295.0
52	88198.5
53	84729.5
54	82257.0
55	76751.0
56	69576.5
57	62924.5
58	57460.0
59	52259.5
60	45072.0
61	37093.5
62	29368.5
63	21463.5
64	15261.5
65	11046.0
66	7666.5
67	5263.0
68	3744.5
69	2767.5
70	2139.5
71	1714.0
72	1291.0
73	1095.5
74	852.5
75	564.5
76	397.0
77	232.0
78	127.0
79	72.5
80	38.5
81	21.0
82	12.5
83	8.5
84	5.5
85	4.0
86	4.0
87	3.5
88	2.5
89	1.5
90	0.5
91	1.0
92	2.0
93	2.5
94	1.5
95	2.0
96	5.5
97	6.0
98	3.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07477067054586421
2	1.9159684957300248E-4
3	8.142866106852605E-4
4	0.18350188267854312
5	0.06705889735055087
6	0.009915136965402879
7	0.014321864505581936
8	0.011016818850447642
9	0.01772270858550273
10-11	0.01777060779789598
12-13	0.018465146377598114
14-15	0.025937423510945207
16-17	0.02634456681628784
18-19	0.024644144776327447
20-21	0.027326500670349477
22-23	0.029122721135096376
24-25	0.026392466028681092
26-27	0.025769776267568833
28-29	0.0272546518517596
30-31	0.02773364397569211
32-33	0.023805908559445556
34-35	0.025961373117141834
36-37	0.029050872316506497
38-39	0.033026506945146306
40-41	0.028619779404967245
42-43	0.028859275466933496
44-45	0.028883225073130122
46-47	0.029841209320995134
48-49	0.026272717997697963
50-51	0.03221222033446104
52-53	0.027997089643854986
54-55	0.03149373214856228
56-57	0.028835325860736873
58-59	0.02512313690025995
60-61	0.026919357365006846
62-63	0.030080705382961388
64-65	0.03556516520198859
66-67	0.03405634001160119
68-69	0.029290368378472754
70-71	0.03872651321994312
72-73	0.04057063289708327
74-75	0.025745826661372206
76-77	0.018441196771401488
78-79	0.02282397470538392
80-81	0.02634456681628784
82-83	0.02495548965688357
84-85	0.017579010948322978
86-87	0.01803405346605886
88-89	0.018201700709435234
90-91	0.019950021961788883
92-93	0.02373405974085568
94-95	0.018513045589991364
96-97	0.021865990457518907
98-99	0.01853699519618799
100-101	0.018249599921828483
102-103	0.016094135364132206
104-105	0.0192075841696935
106-107	0.01614203457652546
108-109	0.0155193448154132
110-111	0.012836988921391165
112-113	0.010633625151301637
114-115	0.009316396810487244
116-117	0.00969959050963325
118-119	0.005843703911976575
120-121	0.004286979509195931
122-123	0.008023118075869478
124-125	0.05551518716377747
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2087717.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.86703433408886
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.02447350695473	26.720669865259943
2	13.304558640361572	10.608265922303868
3	6.321121035616219	7.560130480705294
4	3.574515065016099	5.7002125929885885
5	2.2543767198251867	4.493765704562068
6	1.5519583456030697	3.7123185989539995
7	1.1299954080566499	3.1534696011250056
8	0.7834786108784402	2.498797494393202
9	0.63637007352905	2.283316881351236
>10	3.190403348886859	22.143774015237945
>50	0.1616696583197992	4.423070500330311
>100	0.062359037235170486	4.172973223476521
>500	0.003025993408442285	0.8453302709220515
>1k	0.0015735165723899873	1.4273332619935695
>5k	1.2103973633769133E-4	0.25657158639642347
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5317	0.25468011229491355	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4982	0.23863387614317458	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	4430	0.21219351090210026	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	4156	0.19906912670634958	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3034	0.14532621040112237	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	4.789921239325062E-5	0.0
12-13	0.0	0.0	0.0	4.789921239325062E-5	0.0
14-15	0.0	0.0	0.0	7.184881858987593E-5	0.0
16-17	0.0	0.0	0.0	1.9159684957300248E-4	0.0
18-19	0.0	0.0	0.0	2.634456681628784E-4	0.0
20-21	0.0	0.0	0.0	4.3109291153925553E-4	0.0
22-23	0.0	0.0	0.0	8.621858230785111E-4	0.0
24-25	0.0	0.0	0.0	0.0021315149514996527	0.0
26-27	0.0	0.0	0.0	0.004143281872016178	0.0
28-29	0.0	0.0	0.0	0.009220598385700744	0.0
30-31	0.0	0.0	0.0	0.02251262982482779	0.0
32-33	0.0	0.0	0.0	0.04756391790649786	0.0
34-35	0.0	0.0	0.0	0.08183580437386867	0.0
36-37	0.0	0.0	0.0	0.13663250335174737	0.0
38-39	0.0	0.0	0.0	0.22194100062412675	0.0
40-41	0.0	0.0	0.0	0.3215474127958914	0.0
42-43	0.0	0.0	0.0	0.40582607700181583	0.0
44-45	0.0	0.0	0.0	0.501121560058188	0.0
46-47	0.0	0.0	0.0	0.6218515248953762	0.0
48-49	0.0	0.0	0.0	0.7440424157105585	0.0
50-51	0.0	0.0	0.0	0.8698736466676278	0.0
52-53	0.0	0.0	0.0	1.0000158067400897	0.0
54-55	0.0	0.0	0.0	1.1432584014021057	0.0
56-57	0.0	0.0	0.0	1.3080316920348878	0.0
58-59	0.0	0.0	0.0	1.4899768503106503	0.0
60-61	0.0	0.0	0.0	1.6769753754939007	0.0
62-63	0.0	0.0	0.0	1.8685482754607066	0.0
64-65	0.0	0.0	0.0	2.073293458835656	0.0
66-67	0.0	0.0	0.0	2.315879020001274	0.0
68-69	0.0	0.0	0.0	2.5943410912494365	0.0
70-71	0.0	0.0	0.0	2.9076019403012956	0.0
72-73	0.0	0.0	0.0	3.2127678224586953	0.0
74-75	0.0	0.0	0.0	3.519274882563106	0.0
76-77	0.0	0.0	0.0	3.869298377126785	0.0
78-79	0.0	0.0	0.0	4.258766873096306	0.0
80-81	0.0	0.0	0.0	4.681357674435759	0.0
82-83	0.0	0.0	0.0	5.125239675684012	0.0
84-85	0.0	0.0	0.0	5.574390590295524	0.0
86-87	0.0	0.0	0.0	6.05764574413103	0.0
88-89	0.0	0.0	0.0	6.600463568577542	0.0
90-91	0.0	0.0	0.0	7.164883937813411	0.0
92-93	0.0	0.0	0.0	7.741686253452935	0.0
94-95	0.0	0.0	0.0	8.333073879266202	0.0
96-97	0.0	0.0	0.0	8.945225813651946	0.0
98-99	0.0	0.0	0.0	9.619215631237376	0.0
100-101	0.0	0.0	0.0	10.359138714682114	0.0
102-103	0.0	0.0	0.0	11.095732802865523	0.0
104-105	0.0	0.0	0.0	11.84403824847908	0.0
106-107	0.0	0.0	0.0	12.624124821515561	0.0
108-109	0.0	0.0	0.0	13.431681592859569	0.0
110-111	0.0	0.0	0.0	14.271594282175219	0.0
112-113	0.0	0.0	0.0	15.134785988713986	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	15120	0.0	28.685736	1
GGTATCA	11470	0.0	28.08754	1
TATCAAC	17710	0.0	24.078243	2
CATGGGG	7640	0.0	23.812004	4
ATCAACG	17940	0.0	23.638275	3
TCAACGC	17895	0.0	23.598862	4
CAACGCA	17950	0.0	23.446018	5
GAGTACT	8450	0.0	23.444527	12-13
AACGCAG	17990	0.0	23.379875	6
AGTACTT	8425	0.0	23.05511	12-13
GTACTTT	8790	0.0	22.470812	14-15
GGGTATA	455	0.0	22.290556	1
GTAAGTA	1255	0.0	21.384844	2
ACGCAGA	19965	0.0	20.888279	7
GTAGCCC	1205	0.0	20.788868	3
GTACATG	17030	0.0	20.739124	1
ATGGGGG	4135	0.0	20.730085	5
TACATGG	16690	0.0	20.725689	2
CGCAGAG	20165	0.0	20.680609	8
GTGTAGC	1490	0.0	20.420542	1
>>END_MODULE