FastQCFastQC Report
Mon 15 Aug 2016
SRR2921109_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921109_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2470921
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA36240.14666595977775088No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT35700.1444805398472877No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG65750.025.8825534
GTATCAA106150.024.6202451
TACATGG139650.022.6690792
GTACATG145800.022.4978961
TCAACGC119750.021.1675664
TATCAAC120800.021.1326312
ACATGGG146100.021.0573433
AACGCAG120550.020.9283736
CAACGCA122850.020.6342585
ATCAACG123150.020.5360873
GTGTACG3201.211265E-820.4641
GGTATCA85100.019.3075581
ACGCAGA131200.019.0481287
CGCAGAG133200.018.6731538
GAGTACT36100.018.54459412-13
ATGGGGC32200.017.1862645
GCAGAGT138750.017.1113999
TCTATAC9900.016.8300743
GTATAGG8000.016.37121
TATAGGA10550.016.3571912
Produced by FastQC (version 0.11.5)