##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921109_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2470921
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.40608339967162	31.0	30.0	34.0	25.0	34.0
2	29.473155151459718	31.0	30.0	34.0	23.0	34.0
3	29.578023336237784	31.0	30.0	34.0	23.0	34.0
4	32.997341881832725	37.0	35.0	37.0	26.0	37.0
5	33.10174910488842	37.0	35.0	37.0	26.0	37.0
6	33.08933915734254	37.0	35.0	37.0	26.0	37.0
7	33.01553428863165	37.0	35.0	37.0	26.0	37.0
8	32.91895532070835	37.0	35.0	37.0	25.0	37.0
9	34.30037139997596	39.0	35.0	39.0	25.0	39.0
10-11	34.24371297180282	38.5	35.0	39.0	25.0	39.0
12-13	34.21740456291399	39.0	35.0	39.0	25.0	39.0
14-15	35.24776105751661	40.0	35.0	41.0	25.0	41.0
16-17	35.15367205183816	39.0	35.0	41.0	25.0	41.0
18-19	35.237027205645184	39.5	35.0	41.0	25.0	41.0
20-21	35.27391871290098	40.0	35.0	41.0	25.0	41.0
22-23	35.25643919817752	40.0	35.0	41.0	25.0	41.0
24-25	35.177547562224774	39.5	35.0	41.0	24.5	41.0
26-27	35.11476004291517	39.0	34.5	41.0	24.5	41.0
28-29	34.975278246451424	39.0	34.0	41.0	24.0	41.0
30-31	34.86132033359222	39.0	34.0	41.0	24.0	41.0
32-33	34.748272810017	39.0	34.0	41.0	23.5	41.0
34-35	34.635233582943364	39.0	34.0	41.0	23.0	41.0
36-37	34.49160576157635	39.0	34.0	41.0	21.5	41.0
38-39	34.334993915224324	39.0	33.5	41.0	19.5	41.0
40-41	34.22235656259346	39.0	33.0	41.0	19.0	41.0
42-43	34.126510115054266	39.0	33.0	41.0	18.5	41.0
44-45	33.96667436959741	38.5	33.0	41.0	17.0	41.0
46-47	33.730328488850915	38.0	33.0	40.5	15.0	41.0
48-49	33.58806959024591	38.0	33.0	40.0	13.5	41.0
50-51	33.437237774902556	38.0	33.0	40.0	10.5	41.0
52-53	33.23376526404527	38.0	32.5	40.0	8.5	41.0
54-55	32.99043959721901	38.0	32.0	40.0	8.0	41.0
56-57	32.73390245985202	38.0	32.0	40.0	7.0	41.0
58-59	32.51820414331337	37.0	31.0	40.0	3.5	41.0
60-61	32.22662642795945	37.0	31.0	40.0	2.0	41.0
62-63	31.953301218452552	36.0	31.0	40.0	2.0	41.0
64-65	31.643464117225925	36.0	31.0	39.0	2.0	41.0
66-67	31.354749301980924	35.5	30.5	39.0	2.0	41.0
68-69	30.991089354940932	35.0	30.0	39.0	2.0	40.0
70-71	30.6591222463203	35.0	30.0	38.0	2.0	40.0
72-73	30.292107882040746	35.0	29.0	37.0	2.0	39.5
74-75	30.220885451214343	35.0	30.0	37.0	2.0	39.0
76-77	29.954597496237234	35.0	30.0	36.5	2.0	39.0
78-79	29.689480359752498	35.0	29.5	36.0	2.0	39.0
80-81	29.340708585988786	34.0	29.0	36.0	2.0	37.0
82-83	29.080681049697663	34.0	29.0	35.0	2.0	37.0
84-85	28.88105791322345	34.0	29.0	35.0	2.0	36.5
86-87	28.66365982562777	34.0	29.0	35.0	2.0	36.0
88-89	28.502405580753088	34.0	29.0	35.0	2.0	36.0
90-91	28.339967364395704	34.0	29.0	35.0	2.0	35.5
92-93	28.49483047009597	34.0	29.0	35.0	2.0	35.0
94-95	28.468744043213036	34.0	29.0	35.0	2.0	35.0
96-97	28.404319280138864	34.0	29.0	35.0	2.0	35.0
98-99	28.2932432886361	34.0	29.0	35.0	2.0	35.0
100-101	28.190582377987802	34.0	29.0	35.0	2.0	35.0
102-103	26.91051089856778	32.5	26.0	34.5	2.0	35.0
104-105	27.89229117401973	34.0	28.0	35.0	2.0	35.0
106-107	28.04810291385277	34.0	29.0	35.0	2.0	35.0
108-109	27.99091027191885	34.0	29.0	35.0	2.0	35.0
110-111	27.969386516201855	34.0	29.0	35.0	2.0	35.0
112-113	27.890402606963153	34.0	29.0	35.0	2.0	35.0
114-115	27.756534304415236	34.0	29.0	35.0	2.0	35.0
116-117	27.59361387919727	34.0	27.5	35.0	2.0	35.0
118-119	27.474021022930316	34.0	27.0	35.0	2.0	35.0
120-121	27.298651393549207	34.0	27.0	35.0	2.0	35.0
122-123	27.145328806546225	34.0	27.0	35.0	2.0	35.0
124-125	26.120127474735128	33.0	23.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	105679.0
3	15110.0
4	9926.0
5	9377.0
6	11802.0
7	12424.0
8	12216.0
9	13149.0
10	14482.0
11	15799.0
12	16822.0
13	17659.0
14	18077.0
15	15673.0
16	12127.0
17	10706.0
18	9880.0
19	9458.0
20	9284.0
21	10117.0
22	12000.0
23	16179.0
24	22104.0
25	28837.0
26	32905.0
27	37419.0
28	44143.0
29	52277.0
30	61460.0
31	73270.0
32	91282.0
33	116067.0
34	153841.0
35	220051.0
36	371178.0
37	581932.0
38	206004.0
39	205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.58631386772632	19.44736931627656	13.811757768774571	24.154559047222552
2	15.525225967617379	20.36901973037688	39.574931558079705	24.53082274392603
3	18.957900386727406	24.78374595320574	29.26648894098865	26.9918647190782
4	12.727899764176719	16.917831008697046	36.716138553665786	33.638130673460445
5	13.737670799572099	37.953841464969464	34.299067576707266	14.009420158751167
6	32.09473502290625	36.43289838642373	17.38766344911995	14.084703141550065
7	27.67159182067723	32.806617347669786	21.20861223336698	18.313178598286004
8	25.553139295949713	35.673583968663905	20.029173198055176	18.74410353733121
9	25.92214848110293	16.732596569702356	19.739469630557803	37.60578531863691
10-11	24.547991557031523	26.85592515466296	27.655922726196476	20.940160562109043
12-13	25.083511871768827	24.57872720359129	27.47271938776646	22.865041536873424
14-15	22.792101260002788	25.90494338051809	25.094359057589227	26.20859630188989
16-17	22.572952875292028	28.62113237239318	26.09704545712116	22.708869295193626
18-19	22.468917399274847	27.91449150688513	27.636139858600195	21.980451235239826
20-21	23.670057860087468	26.875818369110526	27.33232413791708	22.12179963288493
22-23	23.280645984442092	26.693461943105433	26.83987002766475	23.18602204478772
24-25	22.845288041972662	26.89543846612892	27.11342044113222	23.145853050766206
26-27	22.815918855651645	26.994005316376185	27.09156260016772	23.098513227804453
28-29	22.82925891447768	27.0411421864249	27.30983122192091	22.819767677176507
30-31	23.19538560728123	26.975430135531496	27.049677613363492	22.77950664382378
32-33	22.70790517641212	27.172318810196604	27.000900900171683	23.118875113219598
34-35	22.84401724017208	27.148171338072263	27.113910361304566	22.893901060451096
36-37	23.051320718673	26.967054075318845	27.013092243084763	22.968532962923387
38-39	23.030701855563958	27.116410949401693	26.935970112829654	22.916917082204694
40-41	22.977507638394105	27.15690664819031	26.953620848713157	22.911964864702433
42-43	23.039214892723212	26.987981697342423	26.900867619788432	23.071935790145933
44-45	23.172829560684235	27.0466168584306	26.811801399220077	22.96875218166509
46-47	22.910061721812117	27.010279279007644	26.9159997725474	23.163659226632838
48-49	22.9102653970433	27.203965900187143	26.777637296103713	23.108131406665848
50-51	22.82325081986653	27.360391997989336	26.67782756313772	23.138529619006416
52-53	22.938136871703392	27.223196371241503	26.48848002735937	23.35018672969574
54-55	22.934754827989742	27.217046603636476	26.487533624882197	23.360664943491578
56-57	23.076155676551608	27.132120364428943	26.431317269822348	23.360406689197106
58-59	23.00271685791517	26.97635932945049	26.71153347263655	23.30939033999779
60-61	23.11653174114131	27.083170178170494	26.650052664968843	23.150245415719358
62-63	22.88060840441361	27.093965189803615	26.727958503458215	23.29746790232456
64-65	22.888088352067836	27.131722708291345	26.721842189828404	23.25834674981241
66-67	23.045293138239707	27.15886472927481	26.52512341746793	23.27071871501755
68-69	23.243164159134356	27.062549792976565	26.477853520774485	23.2164325271146
70-71	23.004608477704917	26.976369966825597	26.690540742350606	23.328480813118883
72-73	23.13138994542012	26.85391889671428	26.664127753985028	23.35056340388057
74-75	23.0582655903334	27.089233780330556	26.541023401587626	23.311477227748416
76-77	23.011539836790643	26.850907704576777	26.757519753625793	23.380032705006787
78-79	23.15897318012935	26.918073317507858	26.70497373336671	23.217979768996084
80-81	23.10749877626379	26.888261906057938	26.65484649117038	23.3493928265079
82-83	23.10183126048951	26.858972828350236	26.690209844831138	23.348986066329115
84-85	23.146877061487466	26.768275655527717	26.574055712267953	23.51079157071686
86-87	23.025570274343604	26.72266074605531	26.669360674341014	23.582408305260074
88-89	23.171157637172534	26.631830803170153	26.75281807876496	23.44419348089235
90-91	23.11829697681007	26.66317940831276	26.77546642923631	23.44305718564086
92-93	22.99128083215764	26.740272081422066	26.868971272559794	23.399475813860498
94-95	22.892292798852555	26.778149034920297	26.842275881830147	23.487282284397
96-97	23.0180546061687	26.74002880739878	26.736608991162303	23.50530759527021
98-99	23.003685522330162	26.758737609953037	26.606261846719264	23.631315020997544
100-101	22.800981995534396	26.935974813902863	26.751061460924763	23.51198172963798
102-103	22.79211993639418	26.89891668551988	26.796441538373745	23.512521839712193
104-105	22.780258749574024	27.018126878435623	26.581852237690857	23.619762134299496
106-107	22.71191400380571	27.10752418131988	26.640137722778068	23.54042409209634
108-109	22.669040715970958	27.142786599061036	26.588083961688707	23.600088723279296
110-111	22.591195991875114	27.324930388639736	26.565443583076163	23.518430036408983
112-113	22.552583544800736	27.36222241109263	26.47596151900374	23.6092325251029
114-115	22.55997610426699	27.387731742874788	26.477455687257308	23.57483646560091
116-117	22.681436476744246	27.31694464781567	26.423892011294893	23.577726864145188
118-119	22.69051660866808	27.218615452264533	26.44808446822981	23.64278347083757
120-121	22.649296605192724	27.471231012751723	26.33682248240754	23.542649899648012
122-123	22.64407438172894	27.41306061246852	26.341913531594223	23.60095147420831
124-125	22.618221129854895	27.48739493075313	26.230918225828404	23.66346571356357
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	855.0
1	611.0
2	334.5
3	251.0
4	191.0
5	175.0
6	180.0
7	215.0
8	265.0
9	341.5
10	379.5
11	388.0
12	443.5
13	511.0
14	608.5
15	719.0
16	836.0
17	996.0
18	1179.5
19	1420.5
20	1772.5
21	2197.5
22	2729.0
23	3530.0
24	4547.5
25	5863.0
26	7883.5
27	10696.5
28	13595.5
29	17137.0
30	21373.0
31	26547.5
32	32824.0
33	38333.0
34	43351.0
35	48517.5
36	55092.0
37	61967.5
38	66521.0
39	70899.5
40	75847.0
41	79690.5
42	82269.5
43	86226.5
44	90741.0
45	93745.0
46	96714.5
47	100198.5
48	103032.5
49	104951.5
50	108429.5
51	105083.0
52	94487.5
53	88168.5
54	84559.5
55	79385.0
56	72551.0
57	65061.5
58	58576.5
59	52684.5
60	44938.0
61	36943.5
62	30596.5
63	23390.5
64	16745.0
65	12592.5
66	9345.0
67	6932.5
68	5250.5
69	3982.0
70	3013.5
71	2239.0
72	1685.0
73	1322.0
74	1051.0
75	774.5
76	537.5
77	373.5
78	274.5
79	174.5
80	98.0
81	65.5
82	46.0
83	38.0
84	37.0
85	39.0
86	28.0
87	19.0
88	13.5
89	9.5
90	8.5
91	7.0
92	10.0
93	12.0
94	10.5
95	7.5
96	7.0
97	9.0
98	10.0
99	9.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0678694300627175
2	0.008620267503493637
3	0.01809042053550073
4	0.017281005746440294
5	0.010603333736691704
6	0.00801320641169831
7	0.008215560108963418
8	0.006475318312483483
9	0.004654135037097503
10-11	0.00914638711638292
12-13	0.0066574366400220806
14-15	0.006981202555646254
16-17	0.011534160744111203
18-19	0.005079077801354232
20-21	0.012384046272624662
22-23	0.0035411897021394045
24-25	0.003015070089250122
26-27	0.002630598064446415
28-29	0.00894403341911781
30-31	0.00526119612889283
32-33	0.0023877736277282844
34-35	0.007284733101543918
36-37	0.005868257220688157
38-39	0.0011331807046846096
40-41	6.272964615218374E-4
42-43	9.308270074195007E-4
44-45	0.0018009479056594687
46-47	0.0030960115681561654
48-49	0.0023068321488222405
50-51	0.003723308029678003
52-53	0.004188721533387753
54-55	0.003824484878310557
56-57	0.0016795356873004032
58-59	0.002104478451557132
60-61	0.004593428927917971
62-63	0.002610362694719904
64-65	0.0031971884167887195
66-67	0.001578358838667849
68-69	0.002650833434172926
70-71	0.0016188295781208709
72-73	0.001902124754292023
74-75	0.0025496565855403714
76-77	0.0018414186451124906
78-79	4.249427642567285E-4
80-81	1.8211832753859796E-4
82-83	0.0
84-85	4.451781339832394E-4
86-87	2.0235369726510886E-4
88-89	0.0
90-91	8.49885528513457E-4
92-93	0.001740241796479936
94-95	0.0014974173597618054
96-97	0.0011331807046846096
98-99	0.0014771819900352944
100-101	0.00137600514140274
102-103	0.003156717677335698
104-105	0.004654135037097503
106-107	0.006495553682209994
108-109	0.012869695146060922
110-111	0.009692742098998713
112-113	0.003682837290224981
114-115	0.00756802827771507
116-117	0.002832951761711524
118-119	0.0011129453349580985
120-121	0.004775547255456569
122-123	7.487086798809027E-4
124-125	0.08389584288611412
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2470921.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.52352678813821
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.63444213928625	32.14227222807153
2	14.46328291819516	13.746924264145392
3	6.459512205110175	9.209364039535775
4	3.567174460111358	6.780988440522583
5	2.120787039893616	5.0393639851160295
6	1.4055639558586441	4.0078413785213876
7	0.9632741030367228	3.204472784798985
8	0.6837925834514137	2.5996988125746796
9	0.49621451165661945	2.122367727363865
>10	2.118524993217989	16.506564539943682
>50	0.06580899856962907	2.093442404282349
>100	0.020081265428089527	1.574712317949594
>500	7.70413092208991E-4	0.2750837741541226
>1k	7.704130922059538E-4	0.6969033030200219
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	3624	0.14666595977775088	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	3570	0.1444805398472877	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.047073945302177E-5	0.0
5	0.0	0.0	0.0	4.047073945302177E-5	0.0
6	0.0	0.0	0.0	4.047073945302177E-5	0.0
7	0.0	0.0	0.0	4.047073945302177E-5	0.0
8	0.0	0.0	0.0	4.047073945302177E-5	0.0
9	0.0	0.0	0.0	4.047073945302177E-5	0.0
10-11	0.0	0.0	2.0235369726510884E-5	8.094147890604353E-5	0.0
12-13	0.0	0.0	4.047073945302177E-5	1.0117684863255441E-4	0.0
14-15	0.0	0.0	4.047073945302177E-5	1.6188295781208707E-4	0.0
16-17	0.0	0.0	4.047073945302177E-5	2.2258906699161972E-4	0.0
18-19	0.0	0.0	4.047073945302177E-5	2.630598064446415E-4	0.0
20-21	0.0	0.0	4.047073945302177E-5	4.249427642567286E-4	0.0
22-23	0.0	0.0	4.047073945302177E-5	6.8800257070137E-4	0.0
24-25	0.0	0.0	4.047073945302177E-5	0.0013355344019497185	0.0
26-27	0.0	0.0	4.047073945302177E-5	0.0027520102828054805	0.0
28-29	0.0	0.0	4.047073945302177E-5	0.005928963329867689	0.0
30-31	0.0	0.0	4.047073945302177E-5	0.014953938227891542	0.0
32-33	0.0	0.0	4.047073945302177E-5	0.03437989316534199	0.0
34-35	0.0	0.0	4.047073945302177E-5	0.06157623007777262	0.0
36-37	0.0	0.0	4.047073945302177E-5	0.10714628270187512	0.0
38-39	0.0	0.0	8.094147890604353E-5	0.17181852434780392	0.0
40-41	0.0	0.0	8.094147890604353E-5	0.24464561999351658	0.0
42-43	0.0	0.0	8.094147890604353E-5	0.30947974459725747	0.0
44-45	0.0	0.0	8.094147890604353E-5	0.3861515604910072	0.0
46-47	0.0	0.0	8.094147890604353E-5	0.48073167859271904	0.0
48-49	0.0	0.0	8.094147890604353E-5	0.5787922802873908	0.0
50-51	0.0	0.0	8.094147890604353E-5	0.6766505282847974	0.0
52-53	4.047073945302177E-5	0.0	8.094147890604353E-5	0.7764513717759491	0.0
54-55	4.047073945302177E-5	0.0	8.094147890604353E-5	0.8892230872617943	0.0
56-57	4.047073945302177E-5	0.0	8.094147890604353E-5	1.0217647589704406	0.0
58-59	4.047073945302177E-5	0.0	8.094147890604353E-5	1.1640194081478121	0.0
60-61	4.047073945302177E-5	0.0	8.094147890604353E-5	1.3089451261290832	0.0
62-63	4.047073945302177E-5	0.0	8.094147890604353E-5	1.455813439604099	0.0
64-65	4.047073945302177E-5	0.0	8.094147890604353E-5	1.6221886494954716	0.0
66-67	4.047073945302177E-5	0.0	8.094147890604353E-5	1.8115107686567073	0.0
68-69	4.047073945302177E-5	0.0	8.094147890604353E-5	2.0265520427403385	0.0
70-71	4.047073945302177E-5	0.0	8.094147890604353E-5	2.261221625458685	0.0
72-73	4.047073945302177E-5	0.0	8.094147890604353E-5	2.5021237020527973	0.0
74-75	4.047073945302177E-5	0.0	8.094147890604353E-5	2.7506747484035303	0.0
76-77	4.047073945302177E-5	0.0	8.094147890604353E-5	3.0344758088178456	0.0
78-79	4.047073945302177E-5	0.0	8.094147890604353E-5	3.3496821630477056	0.0
80-81	4.047073945302177E-5	0.0	8.094147890604353E-5	3.6804899873367054	0.0
82-83	4.047073945302177E-5	0.0	8.094147890604353E-5	4.029327526052027	0.0
84-85	4.047073945302177E-5	0.0	8.094147890604353E-5	4.394656891094455	0.0
86-87	4.047073945302177E-5	0.0	8.094147890604353E-5	4.7889430702155185	0.0
88-89	4.047073945302177E-5	0.0	8.094147890604353E-5	5.229831305816738	0.0
90-91	4.047073945302177E-5	0.0	8.094147890604353E-5	5.691238206320639	0.0
92-93	4.047073945302177E-5	0.0	8.094147890604353E-5	6.171221176233477	0.0
94-95	4.047073945302177E-5	0.0	8.094147890604353E-5	6.667736443212875	0.0
96-97	4.047073945302177E-5	0.0	8.094147890604353E-5	7.190476749357831	0.0
98-99	4.047073945302177E-5	0.0	8.094147890604353E-5	7.763501949273166	0.0
100-101	4.047073945302177E-5	0.0	8.094147890604353E-5	8.381611552939166	0.0
102-103	4.047073945302177E-5	0.0	8.094147890604353E-5	8.999377155319818	2.0235369726510884E-5
104-105	4.047073945302177E-5	0.0	8.094147890604353E-5	9.62044112296589	4.047073945302177E-5
106-107	4.047073945302177E-5	0.0	8.094147890604353E-5	10.264613882839637	4.047073945302177E-5
108-109	4.047073945302177E-5	0.0	8.094147890604353E-5	10.947112432975397	4.047073945302177E-5
110-111	4.047073945302177E-5	0.0	8.094147890604353E-5	11.666439356013406	4.047073945302177E-5
112-113	4.047073945302177E-5	0.0	8.094147890604353E-5	12.4170501606486	4.047073945302177E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGGGG	6575	0.0	25.882553	4
GTATCAA	10615	0.0	24.620245	1
TACATGG	13965	0.0	22.669079	2
GTACATG	14580	0.0	22.497896	1
TCAACGC	11975	0.0	21.167566	4
TATCAAC	12080	0.0	21.132631	2
ACATGGG	14610	0.0	21.057343	3
AACGCAG	12055	0.0	20.928373	6
CAACGCA	12285	0.0	20.634258	5
ATCAACG	12315	0.0	20.536087	3
GTGTACG	320	1.211265E-8	20.464	1
GGTATCA	8510	0.0	19.307558	1
ACGCAGA	13120	0.0	19.048128	7
CGCAGAG	13320	0.0	18.673153	8
GAGTACT	3610	0.0	18.544594	12-13
ATGGGGC	3220	0.0	17.186264	5
GCAGAGT	13875	0.0	17.111399	9
TCTATAC	990	0.0	16.830074	3
GTATAGG	800	0.0	16.3712	1
TATAGGA	1055	0.0	16.357191	2
>>END_MODULE