FastQCFastQC Report
Mon 15 Aug 2016
SRR2921109_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921109_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2470921
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40170.16257096038278843No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37440.1515224485121135No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA35410.1433068884031501No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT34110.13804569227425725No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA121700.030.8421041
GGTATCA91050.027.6577221
TATCAAC145350.025.2813192
TCAACGC145050.024.9644854
ATCAACG146400.024.814773
CAACGCA146000.024.7483045
AACGCAG147950.024.4460146
GAGTACT69600.022.94897712-13
CATGGGG65900.022.2690334
ACGCAGA163600.021.8166187
CGCAGAG165950.021.4718288
AGTACTT76050.021.08083512-13
GTACTTT76250.020.94794814-15
TACATGG139850.020.8583742
GTACATG144950.020.8312911
GCAGAGT172350.019.8469419
ACATGGG144750.019.6165393
GTGTAGC15850.019.5775341
AGAGTAC158550.018.2536310-11
TACTTTT91000.017.8140314-15
Produced by FastQC (version 0.11.5)