##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921109_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2470921
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.619441900408795	31.0	31.0	34.0	27.0	34.0
2	30.743951749165596	31.0	31.0	34.0	27.0	34.0
3	30.809494111709764	31.0	31.0	34.0	27.0	34.0
4	34.30702559895683	37.0	35.0	37.0	30.0	37.0
5	33.95040594175208	37.0	35.0	37.0	30.0	37.0
6	33.800322228027525	37.0	35.0	37.0	29.0	37.0
7	33.62369173275876	37.0	35.0	37.0	28.0	37.0
8	33.62409886839765	37.0	35.0	37.0	28.0	37.0
9	35.03492705756275	39.0	35.0	39.0	28.0	39.0
10-11	34.98753926167611	39.0	35.0	39.0	27.5	39.0
12-13	34.8733963975376	39.0	35.0	39.0	27.0	39.0
14-15	35.974839745989456	40.0	36.0	41.0	27.0	41.0
16-17	35.93181226757149	40.0	36.0	41.0	27.0	41.0
18-19	35.9936151337902	40.0	36.0	41.0	27.0	41.0
20-21	36.007377815802286	40.0	36.0	41.0	27.0	41.0
22-23	35.97112756741312	40.0	36.0	41.0	27.0	41.0
24-25	35.877187291702164	40.0	36.0	41.0	27.0	41.0
26-27	35.76888030819278	40.0	35.5	41.0	27.0	41.0
28-29	35.666956774417315	40.0	35.0	41.0	27.0	41.0
30-31	35.56305138853084	39.0	35.0	41.0	26.5	41.0
32-33	35.402515296927746	39.0	35.0	41.0	25.5	41.0
34-35	35.2704473352244	39.0	35.0	41.0	25.0	41.0
36-37	35.12630533311263	39.0	35.0	41.0	25.0	41.0
38-39	34.94963578358029	39.0	34.0	41.0	24.0	41.0
40-41	34.798961399413415	39.0	34.0	41.0	24.0	41.0
42-43	34.6529364961486	39.0	34.0	41.0	23.0	41.0
44-45	34.46188202698508	39.0	34.0	41.0	22.5	41.0
46-47	34.206561642399734	39.0	33.0	41.0	19.5	41.0
48-49	33.98306016258715	39.0	33.0	41.0	17.5	41.0
50-51	33.76851101269527	38.0	33.0	41.0	15.0	41.0
52-53	33.59774391815845	38.0	33.0	40.0	13.5	41.0
54-55	33.40345259925348	38.0	33.0	40.0	12.5	41.0
56-57	33.135758286080375	38.0	32.5	40.0	8.5	41.0
58-59	32.91849921547472	37.5	32.0	40.0	8.0	41.0
60-61	32.663147668419995	37.0	32.0	40.0	7.5	41.0
62-63	32.37233970653048	36.5	31.5	40.0	6.5	41.0
64-65	32.092780586671935	36.0	31.0	39.5	2.0	41.0
66-67	31.767828068967	36.0	31.0	39.0	2.0	41.0
68-69	31.406817741238996	35.0	31.0	39.0	2.0	40.5
70-71	31.044668162195393	35.0	30.5	38.0	2.0	40.0
72-73	30.843046580606988	35.0	30.5	37.5	2.0	40.0
74-75	30.637706952185034	35.0	30.0	37.0	2.0	39.0
76-77	30.390981743244723	35.0	30.0	36.5	2.0	39.0
78-79	30.061148656715453	35.0	30.0	36.0	2.0	39.0
80-81	29.75455063112095	35.0	30.0	36.0	2.0	37.0
82-83	29.52141468707417	35.0	30.0	35.0	2.0	37.0
84-85	29.346127213294153	34.0	30.0	35.0	2.0	36.5
86-87	29.13192509999308	34.0	29.0	35.0	2.0	36.0
88-89	28.981062526887747	34.0	29.0	35.0	2.0	36.0
90-91	28.81529802045472	34.0	29.0	35.0	2.0	36.0
92-93	28.68725892086392	34.0	29.0	35.0	2.0	35.0
94-95	28.581709411187163	34.0	29.0	35.0	2.0	35.0
96-97	28.515780148373825	34.0	29.0	35.0	2.0	35.0
98-99	28.46637610024764	34.0	29.0	35.0	2.0	35.0
100-101	28.322465995472943	34.0	29.0	35.0	2.0	35.0
102-103	26.98633849483654	32.0	26.0	34.0	2.0	35.0
104-105	28.046289015310485	34.0	28.5	35.0	2.0	35.0
106-107	28.30913331506754	34.0	29.0	35.0	2.0	35.0
108-109	28.500330645941332	34.0	29.0	35.0	2.0	35.0
110-111	28.48251684290999	34.0	29.0	35.0	2.0	35.0
112-113	28.41152772589654	34.0	29.0	35.0	2.0	35.0
114-115	28.29944603651837	34.0	29.0	35.0	2.0	35.0
116-117	28.205948105989627	34.0	29.0	35.0	2.0	35.0
118-119	28.07372251075611	34.0	29.0	35.0	2.0	35.0
120-121	27.909512485425473	34.0	29.0	35.0	2.0	35.0
122-123	27.733147680561217	34.0	29.0	35.0	2.0	35.0
124-125	26.966602331681184	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	62546.0
3	20312.0
4	10114.0
5	8358.0
6	9371.0
7	11513.0
8	12901.0
9	14102.0
10	15462.0
11	16764.0
12	18311.0
13	20215.0
14	22829.0
15	19474.0
16	13268.0
17	11714.0
18	11022.0
19	10502.0
20	10301.0
21	10569.0
22	11393.0
23	13831.0
24	18615.0
25	24667.0
26	30717.0
27	36135.0
28	42831.0
29	50819.0
30	60012.0
31	72752.0
32	89038.0
33	112476.0
34	148339.0
35	212401.0
36	367948.0
37	611776.0
38	237095.0
39	428.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.519231688320524	19.91083022343477	14.383932267259835	24.186005820984867
2	15.74065305389018	20.650131894513528	38.96432656094061	24.644888490655685
3	18.61012699006274	24.805435423986868	29.987429691667256	26.59700789428314
4	12.95338174054314	17.247673465813236	36.463773451338916	33.33517134230471
5	13.509129744182486	37.735857462106885	34.57136816970154	14.183644624009084
6	31.940756175144326	36.77037533528341	17.046745600689043	14.242122888883221
7	27.401468162028873	32.734403207030596	21.249815832123087	18.61431279881744
8	25.784430468792372	35.599677169452846	19.81052728719533	18.805365074559447
9	26.043403851571682	16.035356806310695	19.749464879585762	38.17177446253186
10-11	24.793126827836755	26.398081340632874	27.659579635495195	21.149212196035176
12-13	25.621644202085903	23.86977323311655	27.795159939840786	22.713422624956763
14-15	23.066374524974574	25.255048805541918	25.58300990279373	26.095566766689775
16-17	22.556402543622493	28.14066069657139	26.54876123188017	22.754175527925945
18-19	22.371381723891627	27.13211447812402	28.60163861129929	21.894865186685063
20-21	23.415385231953667	26.131248898135834	28.39584162788678	22.057524242023717
22-23	23.03465750460542	26.078132157227806	27.954083628908183	22.93312670925859
24-25	22.62661718741461	26.324364105655235	28.210055536641676	22.838963170288483
26-27	22.625141254316063	26.465011940961315	28.24064169282937	22.669205111893255
28-29	22.596454538940687	26.388691541001602	28.45655330599205	22.55830061406566
30-31	23.026242948825512	26.2950489313912	28.21111432491804	22.467593794865245
32-33	22.607211261425277	26.511302370985103	28.072501628821406	22.808984738768217
34-35	22.67702032443395	26.495155957620575	28.19168907451618	22.636134643429294
36-37	22.87215147110528	26.32589945633968	28.12445791343658	22.67749115911846
38-39	22.92762611888636	26.370294199806484	27.994425304341135	22.707654376966023
40-41	22.837263153207314	26.458506648206736	27.964347232933168	22.73988296565279
42-43	23.038267028174758	26.214518415963035	27.882637389773933	22.864577166088274
44-45	23.043352148944997	26.365956933455404	27.6983386571252	22.892352260474404
46-47	22.92858503365835	26.37494006026663	27.567965939455252	23.12850896661977
48-49	23.02640088006307	26.60723339786724	27.34605727494178	23.020308447127906
50-51	22.891512855972305	26.85635527760415	27.17135817622997	23.080773690193578
52-53	22.95813961019903	26.77595451444903	26.985205860980837	23.280700014371103
54-55	22.90614902584068	26.791430741910784	26.90182865005867	23.40059158218987
56-57	23.06364487780312	26.773626338927247	26.761238750723876	23.40149003254576
58-59	22.86223246659615	26.640337870031118	27.150539266704154	23.34689039666858
60-61	23.032670467483463	26.88277360446285	26.932444519470334	23.15211140858335
62-63	22.729353926273003	26.836323212155293	27.09677118996408	23.337551671607628
64-65	22.64333850729253	26.812646808055828	27.12608023604266	23.41793444860898
66-67	22.86936631478801	26.897219720048987	26.88300962521381	23.35040433994919
68-69	23.072357891596248	26.666924405529024	26.885388453285476	23.375329249589257
70-71	22.82616770970292	26.5870902375653	27.058840200368227	23.52790185236356
72-73	22.976132636948865	26.507044819628323	26.94862140167618	23.568201141746627
74-75	22.853082598533124	26.6654954122721	26.872556457025627	23.608865532169148
76-77	22.76422468046041	26.52334972091326	27.04944426532862	23.66298133329771
78-79	22.885141157906926	26.669254689813688	26.93150455359839	23.514099598681
80-81	22.787703000978635	26.620025482966998	26.900823895291115	23.691447620763253
82-83	22.762392359702023	26.60326738715289	26.8791422982222	23.75519795492289
84-85	22.749116926733002	26.51172853660901	26.75997873297682	23.979175803681173
86-87	22.670309962847206	26.49069095458826	26.861287927192905	23.97771115537163
88-89	22.695546174080537	26.4660612356791	26.865033658554655	23.973358931685713
90-91	22.67238713291786	26.481949686451095	26.900601839033563	23.945061341597487
92-93	22.581394044998195	26.49297557202065	26.935545603273393	23.99008477970776
94-95	22.49046337628862	26.54462136041799	26.911332067180577	24.05358319611281
96-97	22.639116165679468	26.432289057621876	26.79502787535078	24.133566901347876
98-99	22.60223138614112	26.444162218230577	26.650745060338505	24.3028613352898
100-101	22.430607348248955	26.59302293810547	26.720874009923286	24.255495703722293
102-103	22.419745380714602	26.41428173593166	26.789163541808335	24.376809341545396
104-105	22.376359620166518	26.602511039482263	26.555900117023167	24.46522922332805
106-107	22.30643812472121	26.61799099940012	26.6620306318311	24.413540244047567
108-109	22.213173313364567	26.644012912235254	26.630108984932356	24.512704789467826
110-111	22.096795084555044	26.821252499413095	26.578637404981826	24.503315011050038
112-113	22.010028549207668	26.875860225248736	26.437432469598544	24.67667875594505
114-115	21.981391819078695	26.88605904227062	26.41406491108244	24.71848422756824
116-117	22.002428886466255	26.810316756989323	26.422083479463847	24.765170877080575
118-119	21.972939265424966	26.756781423484245	26.39829073908172	24.87198857200907
120-121	21.88175699671991	26.99712666065921	26.314802159689187	24.806314182931686
122-123	21.806834732487737	27.01072582732513	26.240868799905776	24.94157064028136
124-125	21.594672210474656	27.05805367958917	26.24189019664375	25.10538391329242
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	71.0
1	78.0
2	122.0
3	184.0
4	164.0
5	123.5
6	173.0
7	418.5
8	668.5
9	752.0
10	655.0
11	515.0
12	538.5
13	611.5
14	737.5
15	877.5
16	983.5
17	1153.0
18	1362.0
19	1583.5
20	1898.5
21	2349.0
22	2898.0
23	3623.5
24	4547.0
25	5777.0
26	7679.5
27	10539.0
28	13560.0
29	17031.5
30	21490.0
31	26868.0
32	33051.0
33	38634.5
34	43651.0
35	48764.5
36	55116.5
37	61786.0
38	66525.0
39	70899.0
40	75711.5
41	79757.0
42	82524.0
43	86395.5
44	91091.5
45	94341.5
46	97097.5
47	100744.0
48	103768.5
49	105681.5
50	110159.0
51	106057.0
52	94055.0
53	88334.0
54	84817.5
55	79307.5
56	71961.5
57	64241.5
58	58388.5
59	52464.0
60	44397.5
61	36414.5
62	29941.5
63	22767.0
64	16165.5
65	12060.0
66	8907.5
67	6502.0
68	4835.0
69	3553.0
70	2638.0
71	1946.0
72	1516.5
73	1226.5
74	887.0
75	625.0
76	432.5
77	264.0
78	188.0
79	129.5
80	59.5
81	32.5
82	25.0
83	19.5
84	11.5
85	11.0
86	9.5
87	4.5
88	3.5
89	4.5
90	4.5
91	2.0
92	2.5
93	2.0
94	0.0
95	1.0
96	3.0
97	3.5
98	2.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07782523196816085
2	2.832951761711524E-4
3	7.689440496074137E-4
4	0.1814707957073496
5	0.07037861590880486
6	0.010117684863255441
7	0.014124288069104598
8	0.011129453349580987
9	0.018171362014406774
10-11	0.01804994979604771
12-13	0.01928430734936487
14-15	0.026649981929814832
16-17	0.026892806366532965
18-19	0.024181266823180506
20-21	0.027337984500516208
22-23	0.02972575812824449
24-25	0.026710688038994363
26-27	0.026002450098566488
28-29	0.026872570996806452
30-31	0.027378455239969225
32-33	0.02333138129466705
34-35	0.025031152351693963
36-37	0.029159167775902183
38-39	0.03363118448546109
40-41	0.02857234205383337
42-43	0.02887587259973103
44-45	0.029159167775902183
46-47	0.02960434590988542
48-49	0.026042920838019505
50-51	0.03176953047062209
52-53	0.027762927264772932
54-55	0.030980351051288164
56-57	0.02859257742355988
58-59	0.02517279993977954
60-61	0.02681186488762692
62-63	0.030514937547578416
64-65	0.034764365190145696
66-67	0.03423824557725642
68-69	0.029159167775902183
70-71	0.03919591116025158
72-73	0.04132062498153523
74-75	0.02569891955266882
76-77	0.01831300960249235
78-79	0.02294690926986334
80-81	0.026042920838019505
82-83	0.024221737562633527
84-85	0.017382182595072848
86-87	0.01811065590522724
88-89	0.018191597384133285
90-91	0.019952074550339732
92-93	0.02337185203412007
94-95	0.018009479056594686
96-97	0.02219820058998244
98-99	0.019567602525536024
100-101	0.0189605414337407
102-103	0.0165525324362859
104-105	0.01902124754292023
106-107	0.016957239830816123
108-109	0.01602641282339662
110-111	0.013800522153480422
112-113	0.011109217979854476
114-115	0.009449917662280581
116-117	0.009773683577904757
118-119	0.005888492590414667
120-121	0.004148250793934731
122-123	0.008195324739236908
124-125	0.05580914970571702
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2470921.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.04405268269223
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.28031618602867	30.518143702950024
2	14.966239032642362	13.782125969614995
3	6.756573751285325	9.333001132760309
4	3.723866590328058	6.858476378735304
5	2.254188070195111	5.189597713038001
6	1.4695514540536503	4.059846274222394
7	0.9796186852218374	3.157393004591278
8	0.6918158419914442	2.5483204060300113
9	0.5251924261061773	2.17637889625654
>10	2.257274626124477	16.997394412664693
>50	0.06951309090640004	2.173848461673887
>100	0.024260132033637312	1.9460086372729835
>500	6.18377310012999E-4	0.2433593578337712
>1k	9.717357728775693E-4	1.0161056523559144
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4017	0.16257096038278843	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3744	0.1515224485121135	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	3541	0.1433068884031501	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	3411	0.13804569227425725	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	2.0235369726510884E-5	0.0
12-13	0.0	0.0	0.0	6.0706109179532654E-5	0.0
14-15	0.0	0.0	0.0	1.2141221835906531E-4	0.0
16-17	0.0	0.0	0.0	1.4164758808557618E-4	0.0
18-19	0.0	0.0	0.0	1.6188295781208707E-4	0.0
20-21	0.0	0.0	0.0	3.0353054589766327E-4	0.0
22-23	0.0	0.0	0.0	6.070610917953265E-4	0.0
24-25	0.0	0.0	0.0	0.0012950636624966966	0.0
26-27	0.0	0.0	0.0	0.002590127324993393	0.0
28-29	0.0	0.0	0.0	0.005807551111508624	0.0
30-31	0.0	0.0	0.0	0.014569466203087836	0.0
32-33	0.0	0.0	0.0	0.03367165522491411	0.0
34-35	0.0	0.0	0.0	0.06028116641527592	0.0
36-37	0.0	0.0	0.0	0.10508227498977102	0.0
38-39	0.0	0.0	0.0	0.16902604332554544	0.0
40-41	0.0	0.0	0.0	0.24074019363629998	0.0
42-43	0.0	0.0	0.0	0.30468396197207437	0.0
44-45	0.0	0.0	0.0	0.3800404788336009	0.0
46-47	0.0	0.0	0.0	0.4723947062653966	0.0
48-49	0.0	0.0	0.0	0.5683103587690581	0.0
50-51	0.0	0.0	0.0	0.6648533077342416	0.0
52-53	0.0	0.0	0.0	0.7634602644115291	0.0
54-55	0.0	0.0	0.0	0.8753416236294078	0.0
56-57	0.0	0.0	0.0	1.0054550509708728	0.0
58-59	0.0	0.0	0.0	1.1466372255527393	0.0
60-61	0.0	0.0	0.0	1.2892763467549144	4.047073945302177E-5
62-63	0.0	0.0	0.0	1.4347281843490747	4.047073945302177E-5
64-65	0.0	0.0	0.0	1.5983311485879153	4.047073945302177E-5
66-67	0.0	0.0	0.0	1.7848203159874396	4.047073945302177E-5
68-69	0.0	0.0	0.0	1.9981618190140438	4.047073945302177E-5
70-71	0.0	0.0	0.0	2.230848335499193	4.047073945302177E-5
72-73	0.0	0.0	0.0	2.470030405666551	4.047073945302177E-5
74-75	0.0	0.0	0.0	2.7172054468758815	4.047073945302177E-5
76-77	0.0	0.0	0.0	2.9978700249825874	4.047073945302177E-5
78-79	0.0	0.0	0.0	3.3092721297038636	4.047073945302177E-5
80-81	0.0	0.0	0.0	3.637773121844041	4.047073945302177E-5
82-83	0.0	0.0	0.0	3.9840002978646423	4.047073945302177E-5
84-85	0.0	4.047073945302177E-5	0.0	4.3470025953885205	4.047073945302177E-5
86-87	0.0	4.047073945302177E-5	0.0	4.738374881268967	4.047073945302177E-5
88-89	0.0	4.047073945302177E-5	0.0	5.174528040354184	4.047073945302177E-5
90-91	0.0	4.047073945302177E-5	0.0	5.629439387175875	4.047073945302177E-5
92-93	0.0	4.047073945302177E-5	0.0	6.104080219480915	4.047073945302177E-5
94-95	0.0	4.047073945302177E-5	0.0	6.594828406088256	4.047073945302177E-5
96-97	0.0	4.047073945302177E-5	0.0	7.110304214501395	4.047073945302177E-5
98-99	0.0	4.047073945302177E-5	0.0	7.676935037583153	4.047073945302177E-5
100-101	0.0	4.047073945302177E-5	0.0	8.290390506212056	4.047073945302177E-5
102-103	0.0	4.047073945302177E-5	0.0	8.904109034647405	4.047073945302177E-5
104-105	0.0	4.047073945302177E-5	0.0	9.522340050531765	4.047073945302177E-5
106-107	0.0	4.047073945302177E-5	0.0	10.166533045775239	4.047073945302177E-5
108-109	0.0	4.047073945302177E-5	0.0	10.846441468586004	4.047073945302177E-5
110-111	0.0	4.047073945302177E-5	0.0	11.565120859792765	4.047073945302177E-5
112-113	4.047073945302177E-5	4.047073945302177E-5	0.0	12.313890245782847	4.047073945302177E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	12170	0.0	30.842104	1
GGTATCA	9105	0.0	27.657722	1
TATCAAC	14535	0.0	25.281319	2
TCAACGC	14505	0.0	24.964485	4
ATCAACG	14640	0.0	24.81477	3
CAACGCA	14600	0.0	24.748304	5
AACGCAG	14795	0.0	24.446014	6
GAGTACT	6960	0.0	22.948977	12-13
CATGGGG	6590	0.0	22.269033	4
ACGCAGA	16360	0.0	21.816618	7
CGCAGAG	16595	0.0	21.471828	8
AGTACTT	7605	0.0	21.080835	12-13
GTACTTT	7625	0.0	20.947948	14-15
TACATGG	13985	0.0	20.858374	2
GTACATG	14495	0.0	20.831291	1
GCAGAGT	17235	0.0	19.846941	9
ACATGGG	14475	0.0	19.616539	3
GTGTAGC	1585	0.0	19.577534	1
AGAGTAC	15855	0.0	18.25363	10-11
TACTTTT	9100	0.0	17.81403	14-15
>>END_MODULE