FastQCFastQC Report
Mon 15 Aug 2016
SRR2921108_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921108_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4348854
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55600.049.5733031
GTATCAA87300.046.3017271
TCAACGC108750.036.7155534
ATCAACG112900.035.4733
CAACGCA112400.035.416585
TATCAAC115800.034.79022
AACGCAG119150.033.4582026
GTACATG198100.030.2011361
TACATGG202100.028.9443632
ACATGGG202600.028.197373
ACGCAGA142950.027.8460647
CGCAGAG150000.026.338668
CATGGGG152200.026.273114
GCAGAGT168350.022.5488519
GTATAGG11050.021.5496081
AGAGTAC144500.020.6949810-11
CAGAGTA155100.020.29741310-11
ATGGGGA74500.019.7282055
GTGTAGC25650.017.6387521
GTGGTAT32150.017.405581
Produced by FastQC (version 0.11.5)