FastQCFastQC Report
Mon 15 Aug 2016
SRR2921108_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921108_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4348854
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63950.060.8139761
GTATCAA106150.048.999631
TCAACGC141500.036.4106524
ATCAACG146050.035.357583
TATCAAC146900.035.3141372
CAACGCA145550.035.2513665
AACGCAG153000.033.5548676
ACGCAGA177350.028.6459227
GTACATG193100.027.8316441
CGCAGAG187450.026.94478
TACATGG197350.026.5282422
ACATGGG197650.025.8553333
CATGGGG147450.024.3861774
GCAGAGT204950.023.8320479
AGAGTAC183800.022.2857410-11
CAGAGTA196850.021.62433810-11
GTGGTAT35950.020.9058571
GTACTTT86200.020.87777314-15
GAGTACT87050.020.53722812-13
AGTACTT92600.018.95296312-13
Produced by FastQC (version 0.11.5)