FastQCFastQC Report
Mon 15 Aug 2016
SRR2921107_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921107_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3820552
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA90650.034.54541
GGTATCA62650.030.7890431
TCAACGC99000.029.7526864
CAACGCA102500.028.8532285
ATCAACG104550.028.4024263
AACGCAG106150.027.8039516
TATCAAC112000.026.9382172
GTACATG157950.026.874611
TACATGG162000.025.566612
ACATGGG160250.025.1031133
ACGCAGA119650.024.467937
CGCAGAG125100.023.401988
CATGGGG105200.022.795314
GCAGAGT141250.019.546749
GTATAGG11800.018.667751
TATACCG2952.580331E-618.1544195
GTGTAGC26750.018.0274181
AGAGTAC121950.017.93376510-11
CAGAGTA135850.016.49306510-11
GTATAGA13050.016.4234451
Produced by FastQC (version 0.11.5)