##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921107_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3820552
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.276773879795382	31.0	30.0	34.0	23.0	34.0
2	29.339537846887048	31.0	30.0	34.0	23.0	34.0
3	29.450496158670266	31.0	30.0	34.0	23.0	34.0
4	32.89994273078864	35.0	35.0	37.0	26.0	37.0
5	32.964370070084115	37.0	35.0	37.0	26.0	37.0
6	32.95264846545735	37.0	35.0	37.0	26.0	37.0
7	32.87301442304672	37.0	35.0	37.0	25.0	37.0
8	32.77990876711009	37.0	33.0	37.0	25.0	37.0
9	34.16701853554146	39.0	35.0	39.0	25.0	39.0
10-11	34.08443099321774	38.0	34.0	39.0	25.0	39.0
12-13	34.050315896760466	38.0	34.0	39.0	25.0	39.0
14-15	35.05587504109354	39.0	34.5	41.0	24.0	41.0
16-17	34.94894101690018	39.0	34.0	41.0	23.5	41.0
18-19	35.01364671911284	39.0	34.0	41.0	24.0	41.0
20-21	35.04009185060169	39.0	34.0	41.0	24.0	41.0
22-23	35.013966699052915	39.0	34.0	41.0	24.0	41.0
24-25	34.92262387738735	39.0	34.0	41.0	23.5	41.0
26-27	34.856828542053606	39.0	34.0	41.0	23.5	41.0
28-29	34.710611581781905	39.0	34.0	41.0	22.5	41.0
30-31	34.610454327018715	39.0	34.0	41.0	22.0	41.0
32-33	34.50822171769943	39.0	34.0	41.0	21.5	41.0
34-35	34.40125811662817	39.0	34.0	41.0	20.5	41.0
36-37	34.26320542162494	39.0	33.5	41.0	19.0	41.0
38-39	34.10788388693571	39.0	33.0	41.0	18.0	41.0
40-41	33.99396526994005	39.0	33.0	41.0	16.5	41.0
42-43	33.90828210164395	38.5	33.0	41.0	16.0	41.0
44-45	33.75148564919415	38.0	33.0	40.5	15.0	41.0
46-47	33.51798405047229	38.0	33.0	40.0	13.5	41.0
48-49	33.37821772874705	38.0	33.0	40.0	10.5	41.0
50-51	33.209324202366574	38.0	32.0	40.0	9.0	41.0
52-53	33.0036782642927	38.0	32.0	40.0	8.0	41.0
54-55	32.75128698680191	38.0	32.0	40.0	7.0	41.0
56-57	32.48210219360972	37.0	31.0	40.0	4.0	41.0
58-59	32.25828191842436	37.0	31.0	40.0	2.0	41.0
60-61	31.953933096578716	36.5	31.0	40.0	2.0	41.0
62-63	31.67454990797141	36.0	31.0	39.5	2.0	41.0
64-65	31.349119446613997	35.5	30.0	39.0	2.0	41.0
66-67	31.064509395500963	35.0	30.0	39.0	2.0	40.0
68-69	30.710628725901387	35.0	30.0	38.0	2.0	40.0
70-71	30.38682263714772	35.0	29.0	37.5	2.0	40.0
72-73	30.034574061549222	35.0	29.0	37.0	2.0	39.0
74-75	29.96191505834759	35.0	29.0	37.0	2.0	39.0
76-77	29.713602641712505	35.0	29.0	36.0	2.0	39.0
78-79	29.454854822025716	34.0	29.0	36.0	2.0	38.5
80-81	29.1164398495296	34.0	29.0	35.0	2.0	37.0
82-83	28.866836389087233	34.0	29.0	35.0	2.0	37.0
84-85	28.68854251951027	34.0	29.0	35.0	2.0	36.0
86-87	28.486481011120905	34.0	29.0	35.0	2.0	36.0
88-89	28.33699135622287	34.0	29.0	35.0	2.0	36.0
90-91	28.184380162866518	34.0	28.5	35.0	2.0	35.0
92-93	28.3581046665508	34.0	29.0	35.0	2.0	35.0
94-95	28.34084289390643	34.0	29.0	35.0	2.0	35.0
96-97	28.28395176927313	34.0	29.0	35.0	2.0	35.0
98-99	28.177010416295865	34.0	29.0	35.0	2.0	35.0
100-101	28.07845436994445	34.0	29.0	35.0	2.0	35.0
102-103	26.788820306594438	32.5	25.5	34.0	2.0	35.0
104-105	27.787968728079086	34.0	28.0	35.0	2.0	35.0
106-107	27.955539670707267	34.0	29.0	35.0	2.0	35.0
108-109	27.899055162709473	34.0	29.0	35.0	2.0	35.0
110-111	27.870777050017903	34.0	29.0	35.0	2.0	35.0
112-113	27.797207183673983	34.0	29.0	35.0	2.0	35.0
114-115	27.67151278139913	34.0	28.0	35.0	2.0	35.0
116-117	27.51350132127504	34.0	27.0	35.0	2.0	35.0
118-119	27.403102091006744	34.0	27.0	35.0	2.0	35.0
120-121	27.230705013306977	34.0	27.0	35.0	2.0	35.0
122-123	27.084476405503707	34.0	27.0	35.0	2.0	35.0
124-125	26.04875774495413	33.0	23.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	175885.0
3	25399.0
4	16164.0
5	14452.0
6	16241.0
7	17927.0
8	19258.0
9	21133.0
10	22582.0
11	24158.0
12	25079.0
13	25354.0
14	25671.0
15	22630.0
16	18998.0
17	16747.0
18	15464.0
19	14899.0
20	14823.0
21	15953.0
22	19133.0
23	25779.0
24	34689.0
25	45526.0
26	53227.0
27	61363.0
28	72168.0
29	84039.0
30	98779.0
31	119628.0
32	145964.0
33	184532.0
34	244911.0
35	352465.0
36	593213.0
37	861410.0
38	274675.0
39	234.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.66526877081816	18.40701687663916	13.156617505888738	24.771096846653943
2	16.04191201858126	19.434436951222096	39.236001950686784	25.287649079509855
3	19.642396668533245	23.655361680749795	28.61059647067047	28.091645180046488
4	13.207245367582765	15.891272363619228	35.85935986512453	35.04212240367347
5	14.3967544398579	37.0502846645032	33.98060946396584	14.57235143167306
6	33.67925098758513	35.1459369552772	16.93774921947334	14.237062837664327
7	29.14796539402903	31.513160477991704	20.62911142232635	18.709762705652917
8	26.531437603036885	35.089630635191455	19.512914653919104	18.866017107852556
9	26.35259559254855	16.254181932689633	19.210230183312685	38.18299229144914
10-11	25.449511108609734	25.913134984545653	27.293391769504773	21.343962137339837
12-13	25.891484515718822	23.318157105241596	27.30180572944711	23.488552649592474
14-15	23.279622949703942	24.77630635205671	24.546186305357338	27.39788439288201
16-17	23.559979785469963	27.73806817207586	25.36371167252985	23.33824036992433
18-19	23.545510050631073	27.009573244910552	26.691389969197303	22.75352673526107
20-21	24.296985612421373	26.157982081920757	26.48614992186511	23.05888238379276
22-23	24.03153065606239	25.81547925442869	26.30180961381634	23.85118047569258
24-25	23.678108207645476	26.139566854265954	26.44923524563152	23.733089692457053
26-27	23.55812526935992	26.37750319043859	26.380343207562458	23.684028332639034
28-29	23.5272583060218	26.41181428855638	26.494442582955507	23.56648482246631
30-31	23.830432279946045	26.327893852778818	26.261040096809683	23.580633770465457
32-33	23.55738374314866	26.26758314530863	26.23267860601714	23.94235450552557
34-35	23.681479572545015	26.363602691297938	26.42369056277507	23.53122717338198
36-37	23.84384180007159	26.141362212448392	26.297096361077067	23.71769962640295
38-39	23.675232601540852	26.33714803524791	26.297938322965532	23.68968104024571
40-41	23.805552127411612	26.271250657145746	26.405971261087718	23.51722595435492
42-43	23.700990172350636	26.199853867160982	26.296647569236093	23.80250839125229
44-45	23.75501461670876	26.225740300930006	26.261927075992098	23.757318006369136
46-47	23.76857928417667	26.293207698493138	26.218450815415412	23.719762201914783
48-49	23.64142469602946	26.411880738219406	26.171109789563285	23.77558477618785
50-51	23.62110038695438	26.54053414519676	26.10438266471606	23.733982803132804
52-53	23.819933610734	26.422995924584324	25.98425299904079	23.772817465640877
54-55	23.747900057115306	26.27603759035186	26.12030545956357	23.855756892969268
56-57	23.630730580651377	26.438292376745466	26.085298973842995	23.84567806876016
58-59	23.690381975986252	26.219634850625052	26.196064245538437	23.89391892785026
60-61	23.767263829451963	26.297193733651664	26.205055409946176	23.730487026950197
62-63	23.56066933669141	26.453910139646254	26.212589705005108	23.77283081865723
64-65	23.598551937230894	26.35338390637979	26.181936541178974	23.86612761521034
66-67	23.56949043718225	26.390770005134144	26.139101408310246	23.90063814937336
68-69	23.73610346588519	26.212253632867395	26.195645715563042	23.85599718568437
70-71	23.52093196251692	26.164187538951083	26.30636799348357	24.008512505048426
72-73	23.676649789574018	26.1589526613845	26.221876947161793	23.942520601879693
74-75	23.573268475574817	26.300503368309023	26.191014021075453	23.9352141350407
76-77	23.61142957055613	26.17955197205793	26.265051789358186	23.943966668027752
78-79	23.610391136656045	26.184919453235008	26.227662051835804	23.977027358273148
80-81	23.68325172200428	26.20304731153751	26.232768178805767	23.88093278765245
82-83	23.638121831118077	26.17979710256452	26.32454062254371	23.857540443773686
84-85	23.713002949058783	26.210528823012236	26.112964106392262	23.96350412153671
86-87	23.568430682010028	26.130054743498636	26.31416460217063	23.987349972320704
88-89	23.5585090574013	26.04663282075	26.20993393751344	24.184924184335262
90-91	23.51000267764107	26.164728059767672	26.27391456728352	24.05135469530774
92-93	23.59439760102846	26.132959278183144	26.438314338508828	23.834328782279567
94-95	23.45828465952502	26.027280191786367	26.457917430200904	24.056517718487704
96-97	23.547587635120873	25.909219818430213	26.5056708985138	24.037521647935115
98-99	23.388261542593096	25.964388972967427	26.344561526207812	24.30278795823167
100-101	23.330212451194228	26.00338280241256	26.55762948367243	24.10877526272078
102-103	23.26779295809597	25.9230471641516	26.615139496875234	24.194020380877195
104-105	23.250172202605807	26.02551674196727	26.409342379090738	24.31496867633619
106-107	23.186907693775563	26.056205949837047	26.4485845902516	24.308301766135788
108-109	23.265773577743893	25.95858720051308	26.45365601460704	24.321983207135986
110-111	23.16675313769416	26.182986480678043	26.45542257108468	24.194837810543113
112-113	23.109789909692065	26.165520438348732	26.540569991036346	24.18411966092285
114-115	23.2196375725947	26.230433803278487	26.42782830578531	24.1221003183415
116-117	23.045307883001552	26.1036822519586	26.543315060669382	24.307694804370467
118-119	23.176340870498397	26.070324334758755	26.407792148304708	24.345542646438144
120-121	23.058263990137082	26.182123721000846	26.531605577470245	24.228006711391828
122-123	23.05179559421	26.15036756795806	26.46769364782472	24.33014319000722
124-125	23.091565733877463	26.205859273336312	26.42184677566286	24.280728217123368
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	523.0
1	377.0
2	207.5
3	178.5
4	161.5
5	152.5
6	151.5
7	179.0
8	220.5
9	268.5
10	298.5
11	340.0
12	401.0
13	429.0
14	504.5
15	627.5
16	724.0
17	866.5
18	1054.0
19	1222.5
20	1450.0
21	1771.5
22	2387.5
23	3160.0
24	4083.0
25	5476.0
26	7710.5
27	11035.0
28	14892.0
29	19603.0
30	25566.0
31	32306.5
32	40465.0
33	48386.5
34	55563.0
35	63899.0
36	74086.5
37	83894.0
38	90984.5
39	97658.5
40	105863.0
41	113594.5
42	119154.5
43	125923.5
44	134319.5
45	141724.0
46	147669.5
47	151508.0
48	154215.0
49	157823.5
50	161344.5
51	161313.0
52	156899.5
53	152520.5
54	148960.0
55	141375.0
56	130961.5
57	120545.5
58	110644.0
59	99100.0
60	85370.0
61	72376.5
62	59186.0
63	45118.0
64	33215.5
65	24825.0
66	18698.0
67	14157.0
68	10425.0
69	7524.5
70	5680.5
71	4367.0
72	3157.0
73	2357.5
74	1757.0
75	1206.5
76	834.0
77	533.5
78	337.5
79	230.5
80	157.5
81	116.5
82	89.5
83	67.5
84	50.5
85	41.0
86	32.5
87	22.5
88	13.0
89	10.5
90	15.0
91	16.5
92	12.5
93	11.0
94	9.0
95	6.0
96	6.5
97	8.5
98	10.5
99	10.5
100	15.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06991136359353307
2	0.009396547933387636
3	0.019185709290175868
4	0.019159535061949163
5	0.011542834647977571
6	0.00981533558501494
7	0.010522039747136017
8	0.00832340457609267
9	0.006517382828449921
10-11	0.011241831023370445
12-13	0.008454275717226201
14-15	0.009736812900334822
16-17	0.013806905389587683
18-19	0.007053954507097404
20-21	0.014539783779935464
22-23	0.005404978128814894
24-25	0.0045804899396736385
26-27	0.003991569804572742
28-29	0.01130726659393721
30-31	0.007145564305890876
32-33	0.003454998125925259
34-35	0.008205620549072491
36-37	0.006792212224830339
38-39	0.0018321959758694556
40-41	0.0013741469819020917
42-43	0.0014526696665822113
44-45	0.0024996387956504715
46-47	0.004397270342086693
48-49	0.0030362104742979548
50-51	0.005365716786474834
52-53	0.005378803900588187
54-55	0.005496587927608367
56-57	0.0021986351710433466
58-59	0.0030231233601846017
60-61	0.005470413699381661
62-63	0.002957687789617835
64-65	0.003598956381172145
66-67	0.0013479727536753851
68-69	0.002224809399270053
70-71	0.001439582552468858
72-73	0.0022902449698368192
74-75	0.0023295063121768792
76-77	0.0019892413452296943
78-79	1.9630671170029878E-4
80-81	1.0469691290682602E-4
82-83	2.6174228226706506E-5
84-85	2.879165104937716E-4
86-87	1.8321959758694554E-4
88-89	6.543557056676626E-5
90-91	6.543557056676627E-4
92-93	0.0021331996004765805
94-95	0.0013348856395620319
96-97	9.81533558501494E-4
98-99	0.0013087114113353253
100-101	0.0013348856395620319
102-103	0.0028268166484843025
104-105	0.005169410074774536
106-107	0.006870734909510458
108-109	0.012485106864139003
110-111	0.010207949008415538
112-113	0.0032325171859982535
114-115	0.007420393702271294
116-117	0.002381854768630292
118-119	0.001033882014954907
120-121	0.004763709537260584
122-123	8.899237597080211E-4
124-125	0.08379679166780088
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3820552.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.82969394720791
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.10321483478543	34.932217399327236
2	14.450503229366	14.401283066048945
3	5.89200401745902	8.807902707771072
4	3.050295804702141	6.079812255870398
5	1.745183989697725	4.348099204410244
6	1.141914807175517	3.4140759213204572
7	0.7599092402692682	2.650623140919104
8	0.5199923374677643	2.072884722472956
9	0.40403372243975305	1.8119589060171641
>10	1.81983692576718	15.380953166287838
>50	0.07848521711639396	2.6579274240996433
>100	0.033042613693965	2.8000175132159115
>500	0.0013721587262069401	0.4662860786396215
>1k	2.1110133363279104E-4	0.17595849359933521
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.234845645341301E-5
2	0.0	0.0	0.0	0.0	5.234845645341301E-5
3	0.0	0.0	0.0	0.0	5.234845645341301E-5
4	0.0	0.0	0.0	0.0	5.234845645341301E-5
5	0.0	0.0	0.0	0.0	5.234845645341301E-5
6	0.0	0.0	0.0	0.0	5.234845645341301E-5
7	0.0	0.0	0.0	0.0	5.234845645341301E-5
8	0.0	0.0	0.0	0.0	5.234845645341301E-5
9	0.0	0.0	0.0	2.6174228226706506E-5	5.234845645341301E-5
10-11	0.0	0.0	0.0	2.6174228226706506E-5	1.3087114113353253E-4
12-13	0.0	0.0	0.0	2.6174228226706506E-5	4.187876516273041E-4
14-15	0.0	0.0	0.0	2.6174228226706506E-5	4.842232221940704E-4
16-17	0.0	0.0	0.0	2.6174228226706506E-5	5.234845645341301E-4
18-19	0.0	0.0	0.0	5.234845645341301E-5	6.281814774409561E-4
20-21	0.0	0.0	0.0	6.543557056676626E-5	6.936170480077225E-4
22-23	0.0	0.0	0.0	1.9630671170029878E-4	7.19791276234429E-4
24-25	0.0	0.0	0.0	4.187876516273041E-4	7.328783903477822E-4
26-27	0.0	0.0	0.0	6.936170480077225E-4	7.590526185744887E-4
28-29	0.0	0.0	0.0	0.002630509936784004	7.590526185744887E-4
30-31	0.0	0.0	0.0	0.009645203101541348	7.590526185744887E-4
32-33	0.0	0.0	0.0	0.02184239345518658	8.637495314813147E-4
34-35	0.0	0.0	0.0	0.03817511186865144	9.422722161614343E-4
36-37	0.0	0.0	0.0	0.06475504063287191	9.422722161614343E-4
38-39	0.0	0.0	0.0	0.1100495425791875	9.684464443881407E-4
40-41	0.0	0.0	0.0	0.1653164254798783	9.684464443881407E-4
42-43	0.0	0.0	0.0	0.21625147360904917	9.946206726148474E-4
44-45	0.0	0.0	0.0	0.27387403705014357	0.001033882014954907
46-47	0.0	0.0	0.0	0.34120723916334605	0.00108623047140832
48-49	0.0	0.0	0.0	0.4141548132311771	0.001138578927861733
50-51	0.0	0.0	0.0	0.4934496376439844	0.0011778402702017929
52-53	0.0	0.0	0.0	0.5736343858164998	0.0011778402702017929
54-55	0.0	0.0	0.0	0.6639092989704105	0.0011778402702017929
56-57	0.0	0.0	0.0	0.7672058906671078	0.0011778402702017929
58-59	0.0	0.0	0.0	0.8810245221109411	0.0011778402702017929
60-61	0.0	0.0	0.0	1.0040564818905748	0.0012040144984284994
62-63	0.0	0.0	0.0	1.131197795501802	0.0012563629548819123
64-65	0.0	0.0	0.0	1.2678534410734366	0.0012825371831086188
66-67	0.0	0.0	0.0	1.428393069901941	0.0013087114113353253
68-69	0.0	0.0	0.0	1.6101992591646443	0.0013348856395620319
70-71	0.0	0.0	0.0	1.8053281305947413	0.0013610598677887384
72-73	0.0	0.0	0.0	2.0047757496822447	0.0014264954383555045
74-75	0.0	0.0	0.0	2.222283586246176	0.0015050181230356241
76-77	0.0	0.0	0.0	2.4612281157277796	0.0015704536936023904
78-79	0.0	0.0	0.0	2.725640169273969	0.0016228021500558035
80-81	0.0	0.0	0.0	3.007209429422764	0.00164897637828251
82-83	2.6174228226706506E-5	0.0	0.0	3.305176843555591	0.0017798475194160425
84-85	2.6174228226706506E-5	0.0	0.0	3.616336068714678	0.001806021747642749
86-87	2.6174228226706506E-5	0.0	0.0	3.9505024404850397	0.0018714573182095151
88-89	2.6174228226706506E-5	0.0	0.0	4.324178809763615	0.0019368928887762815
90-91	2.6174228226706506E-5	0.0	0.0	4.721281113305093	0.0019368928887762815
92-93	2.6174228226706506E-5	0.0	0.0	5.129245721560654	0.002002328459343048
94-95	2.6174228226706506E-5	0.0	0.0	5.552692385812312	0.002015415573456401
96-97	2.6174228226706506E-5	0.0	0.0	6.002012274666069	0.00217246094281664
98-99	2.6174228226706506E-5	0.0	0.0	6.48463886893831	0.0023295063121768792
100-101	2.6174228226706506E-5	0.0	0.0	7.007050290115146	0.0023556805404035858
102-103	2.6174228226706506E-5	0.0	0.0	7.535887484321638	0.0023818547686302923
104-105	2.6174228226706506E-5	0.0	0.0	8.075639331698666	0.0024342032250837054
106-107	2.6174228226706506E-5	0.0	0.0	8.634642323936436	0.0024342032250837054
108-109	3.926134234005976E-5	0.0	0.0	9.219898067085595	0.0024342032250837054
110-111	5.234845645341301E-5	0.0	0.0	9.838224947599196	0.002499638795650471
112-113	5.234845645341301E-5	0.0	0.0	10.475070094583192	0.002525813023877178
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	9065	0.0	34.5454	1
GGTATCA	6265	0.0	30.789043	1
TCAACGC	9900	0.0	29.752686	4
CAACGCA	10250	0.0	28.853228	5
ATCAACG	10455	0.0	28.402426	3
AACGCAG	10615	0.0	27.803951	6
TATCAAC	11200	0.0	26.938217	2
GTACATG	15795	0.0	26.87461	1
TACATGG	16200	0.0	25.56661	2
ACATGGG	16025	0.0	25.103113	3
ACGCAGA	11965	0.0	24.46793	7
CGCAGAG	12510	0.0	23.40198	8
CATGGGG	10520	0.0	22.79531	4
GCAGAGT	14125	0.0	19.54674	9
GTATAGG	1180	0.0	18.66775	1
TATACCG	295	2.580331E-6	18.154419	5
GTGTAGC	2675	0.0	18.027418	1
AGAGTAC	12195	0.0	17.933765	10-11
CAGAGTA	13585	0.0	16.493065	10-11
GTATAGA	1305	0.0	16.423445	1
>>END_MODULE