FastQCFastQC Report
Mon 15 Aug 2016
SRR2921107_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921107_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3820552
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71000.037.550181
GTATCAA103550.036.6903761
TCAACGC124100.030.1754554
ATCAACG127750.029.4058823
CAACGCA127550.029.1202935
TATCAAC133100.028.3556962
AACGCAG132200.028.2140926
GTACATG154200.024.986821
ACGCAGA151850.024.3671977
TACATGG158650.023.901862
CGCAGAG155400.023.6580438
ACATGGG156800.023.2353763
CATGGGG100650.022.7501054
GCAGAGT174550.020.3478539
AGAGTAC154400.020.01702510-11
GAGTACT77900.018.48146212-13
AGTACTT85600.018.38274412-13
GTACCGT6550.018.1643036
GTACTTT79450.018.12114314-15
CAGAGTA169700.017.87926310-11
Produced by FastQC (version 0.11.5)