FastQCFastQC Report
Mon 15 Aug 2016
SRR2921106_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921106_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5225777
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA103950.033.109241
GTACATG182150.029.5048581
GGTATCA73600.029.3378771
TCAACGC113850.029.1719844
TACATGG179500.028.6484812
ATCAACG116600.028.5350113
AACGCAG117050.028.523736
CAACGCA117150.028.2483525
TATCAAC124150.027.1824532
ACATGGG190250.026.4047813
CATGGGG125700.025.9010184
ACGCAGA133850.024.5415657
CGCAGAG138200.023.553348
GTATACG2951.1296652E-720.2197551
GCAGAGT164650.019.0468049
AGAGTAC134050.018.59836210-11
GTGTAGC40050.018.3189491
ATGGGGG66850.017.0056255
GTATAAT24350.016.9023881
CAGAGTA146900.016.80946210-11
Produced by FastQC (version 0.11.5)