FastQCFastQC Report
Mon 15 Aug 2016
SRR2921106_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921106_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5225777
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71490.13680262284441147No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60010.11483459780239379No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89750.043.73041
GTATCAA138900.040.666781
TCAACGC164550.033.8239634
ATCAACG167550.033.2539063
CAACGCA168500.033.066745
TATCAAC173350.032.2109642
AACGCAG174100.032.0359656
GTACATG182350.028.5889171
TACATGG179750.028.2129572
ACGCAGA199750.027.7250947
CGCAGAG205650.026.872348
ACATGGG190300.025.8652553
CATGGGG126050.023.9212114
GCAGAGT228250.023.3522199
GTATAGG16150.022.5293851
GAGTACT119800.022.14733112-13
AGAGTAC202750.021.35990710-11
CAGAGTA215200.020.64938710-11
GTACTTT129750.020.22095314-15
GTATAGA19550.019.8316381
Produced by FastQC (version 0.11.5)