FastQCFastQC Report
Mon 15 Aug 2016
SRR2921105_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921105_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2657585
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48980.1843026657660997No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36890.1388102356086447No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA32830.1235332077807483No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT31560.11875443306611076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28640.10776701403718038No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA121650.030.9836181
GTACATG178650.030.5453591
TACATGG178300.029.3730752
ACATGGG186300.027.1219623
AACGCAG133450.027.0674466
CATGGGG86650.026.9242574
TATCAAC142550.025.9681642
GGTATCA88900.025.9618931
TCAACGC139450.025.9484464
CAACGCA145550.024.9836145
ATCAACG145250.024.9122923
CGCAGAG147500.024.3250318
ACGCAGA149000.024.199957
TATCCCG1852.6869719E-522.519185
GCAGAGT154700.022.2698029
GAGTACT42550.022.16282512-13
GTATAGA8150.021.9528451
ATGGGAG22700.020.4500795
AGTACTT46150.020.30505812-13
TAAGGCG10000.020.2350945
Produced by FastQC (version 0.11.5)